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Complete nucleotide sequences of a new bipartite begomovirus from Malvastrum sp. plants with bright yellow mosaic symptoms in South Texas

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Abstract

Two isolates of a novel bipartite begomovirus, tentatively named malvastrum bright yellow mosaic virus (MaBYMV), were molecularly characterized from naturally infected plants of the genus Malvastrum showing bright yellow mosaic disease symptoms in South Texas. Six complete DNA-A and five DNA-B genome sequences of MaBYMV obtained from the isolates ranged in length from 2,608 to 2,609 nucleotides (nt) and 2,578 to 2,605 nt, respectively. Both genome segments shared a 178- to 180-nt common region. In pairwise comparisons, the complete DNA-A and DNA-B sequences of MaBYMV were most similar (87-88 % and 79-81 % identity, respectively) and phylogenetically related to the corresponding sequences of sida mosaic Sinaloa virus-[MX-Gua-06]. Further analysis revealed that MaBYMV is a putative recombinant virus, thus supporting the notion that malvaceous hosts may be influencing the evolution of several begomoviruses. The design of new diagnostic primers enabled the detection of MaBYMV in cohorts of Bemisia tabaci collected from symptomatic Malvastrum sp. plants, thus implicating whiteflies as potential vectors of the virus.

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References

  1. Alabi OJ, Ogbe FO, Bandyopadhyay R, Kumar PL, Dixon AGO, Hughes Jd’A, Naidu RA (2008) Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria. Arch Virol 153:1743–1747

    Article  CAS  PubMed  Google Scholar 

  2. Andrade EC, Manhani GG, Alfenas PF, Calegario RF, Fontes EPB, Zerbini FM (2006) Tomato yellow spot virus, a tomato infecting begomovirus from Brazil with a closer relationship to viruses from Sida sp. forms pseudorecombinants with begomoviruses from tomato but not from Sida. J Gen Virol 87:3687–3696

    Article  CAS  PubMed  Google Scholar 

  3. Brown JK, Fauquet CM, Briddon RW, Zerbini M, Moriones E, Navas-Castillo J (2012) Geminiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Ninth report of the ICTV. Elsevier/Academic Press, London, pp 351–373

    Google Scholar 

  4. Brown JK, Zerbini FM, Navas-Castillo J, Moriones E, Ramos-Sobrinho R, Silva JCF, Fiallo-Olivé E, Briddon RW, Hernández-Zepeda C, Idris A, Malathi VG, Martin DP, Rivera-Bustamante R, Ueda S, Varsani A (2015) Revision of Begomovirus taxonomy based on pairwise sequence comparisons. Arch Virol 160:1593–1619

    Article  CAS  PubMed  Google Scholar 

  5. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  6. Fiallo-Olivé E, Zerbini FM, Navas-Castillo J (2015) Complete nucleotide sequences of two new begomoviruses infecting the wild malvaceous plant Melochia sp. in Brazil. Arch Virol 160:3161–3164

    Article  PubMed  Google Scholar 

  7. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  8. Kress WJ, Erickson DL (2007) A two-locus global DNA barcode for land plants: The coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS One 2(6):e508. doi:10.1371/journal.pone.0000508

    Article  PubMed  PubMed Central  Google Scholar 

  9. Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Muhire B, Varsani A, Martin DP (2014) SDT: A virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:e108277

    Article  PubMed  PubMed Central  Google Scholar 

  11. Stewart C, Kon T, Rojas M, Graham A, Martin D, Gilbertson R, Roye M (2014) The molecular characterisation of a Sida-infecting begomovirus from Jamaica. Arch Virol 159:375–378

    Article  CAS  PubMed  Google Scholar 

  12. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Wyatt SD, Brown JK (1996) Detection of Subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86:1288–1293

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported in part by internship subgrant funds provided to the second and fourth authors through a Science, Technology and Environmental Programs for Undergraduate Preparation (STEP UP) to USDA Career Success grant (Prime Award # 2011-38422-30826). We are grateful to Mr. Rabson Mulenga and Ms. Regina Hernandez for technical help. We also thank Dr. Mark H. Mayfield (Kansas State University) for his help with morphological identification of the host plant.

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Authors and Affiliations

  1. Department of Plant Pathology and Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco, TX, 78596, USA

    Olufemi J. Alabi & Lori Gregg

  2. South Texas College, McAllen, TX, 78501, USA

    Cecilia Villegas & K. Daniel Murray

Authors
  1. Olufemi J. Alabi
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  2. Cecilia Villegas
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  3. Lori Gregg
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  4. K. Daniel Murray
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Correspondence to Olufemi J. Alabi.

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Funding

This study was funded by a United States Department of Agriculture STEP UP to Career Success grant (Prime Award # 2011-38422-30826).

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All authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Alabi, O.J., Villegas, C., Gregg, L. et al. Complete nucleotide sequences of a new bipartite begomovirus from Malvastrum sp. plants with bright yellow mosaic symptoms in South Texas. Arch Virol 161, 1729–1733 (2016). https://doi.org/10.1007/s00705-016-2821-z

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  • DOI: https://doi.org/10.1007/s00705-016-2821-z

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