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Genomic characterization and integrative properties of phiSMA6 and phiSMA7, two novel filamentous bacteriophages of Stenotrophomonas maltophilia

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Abstract

Two novel filamentous phages, phiSMA6 and phiSMA7, were isolated from Stenotrophomonas maltophilia environmental strain Khak84. We identified and annotated 11 potential open reading frames in each phage. While the overall layout of the functional gene groups of both phages was similar to that of the known filamentous phages, they differed from them in their molecular structure. The genome of phiSMA6 is a mosaic that evolved by acquiring genes from at least three different filamentous S. maltophilia phages and one Xanthomonas campestris phage related to Cf1. In the phiSMA6 genome, a gene similar to the bacterial gene encoding the mating pair formation protein trbP was also found. We showed that phiSMA6 possesses lysogenic properties and upon induction produces high-titer lysates. The genome of phiSMA7 possesses a unique structure and was found to be closely related to a prophage present in the chromosome of the completely sequenced S. maltophilia clinical strain D457. We suggest that the other three filamentous phages of S. maltophilia described previously also have the capacity to integrate into the genome of their bacterial host.

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Acknowlegements

We are grateful to I.A. Khmel (Institute of Molecular Genetics RAN, Laboratory of Regulation of Expression of Genes of Microorganisms) for kindly providing the 10 strains of Stenotrophomonas maltophilia. We also thank N.A. Khachikian for expert technical assistance. This work was partially supported by state contract #8129 from the Ministry of Education and Science of the Russian Federation and by the Russian Academy of Sciences Presidium Program “Molecular and Cellular Biology” (grant to A. Kulbachinsky).

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Authors and Affiliations

  1. Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov sq., Moscow, 123182, Russia

    Mayya Petrova, Natalya Shcherbatova, Anton Kurakov & Sofia Mindlin

  2. Department of Microbiology, Moscow State University, Moscow, Russia

    Anton Kurakov

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  1. Mayya Petrova
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  2. Natalya Shcherbatova
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Correspondence to Sofia Mindlin.

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Petrova, M., Shcherbatova, N., Kurakov, A. et al. Genomic characterization and integrative properties of phiSMA6 and phiSMA7, two novel filamentous bacteriophages of Stenotrophomonas maltophilia . Arch Virol 159, 1293–1303 (2014). https://doi.org/10.1007/s00705-013-1882-5

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