Abstract
Enoyl–coenzyme A hydratase (ECHS1) is a key enzyme in the metabolism of fatty acids in mitochondria. We previously reported that hepatitis B surface antigen (HBsAg) interacted with ECHS1 in a yeast two-hybrid system. In the current study, we further examined their interaction by using GST pull-down and co-immunoprecipitation assays. The results confirmed that small hepatitis B surface antigen (SHBs) interacted with ECHS1. Furthermore, confocal imaging showed that SHBs and ECHS1 co-localized in HepG2 cells. To clarify the biological function of the interaction, human hepatoma cell lines that transiently and stably expressed SHBs were generated. The expression of SHBs led to a significant decrease in ECHS1 protein levels. ECHS1 protein levels were reduced to 48.44 ± 7.12 % in Huh7 cells transiently expressing SHBs, and to 54.97 ± 3.54 % in HepG2 cells stably expressing SHBs. In conclusion, our findings suggest that SHBs interacts with ECHS1 and regulates ECHS1 protein levels in hepatoma cells.
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This work was supported by Major Projects Foundation of Fujian Medical University (No. 09ZD003)
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Gong, X., Zhu, Y., Dong, J. et al. Small hepatitis B surface antigen interacts with and modulates enoyl–coenzyme A hydratase expression in hepatoma cells. Arch Virol 158, 1065–1070 (2013). https://doi.org/10.1007/s00705-012-1581-7
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DOI: https://doi.org/10.1007/s00705-012-1581-7