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Molecular epidemiology of noroviruses in children and adults with acute gastroenteritis in Wuhan, China, 2007-2010

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Abstract

To study epidemiological features and genetic characteristics of noroviruses in children and adults with acute gastroenteritis, fecal specimens were collected in three hospitals from Jan. 2007 to May 2010 in Wuhan, China. Noroviruses were detected in 25.9 % (286/1103) and 24.6 % (202/822) of the specimens from children and adults, respectively, with genogroup II (GII) being predominant (99.2 %). The most frequent genotype among GII strains was GII.4 (2006b variant) (77.3 %) (72.0 % in children and 87.9 % in adults), followed by GII.3 (15.0 %) and GII.6 (3.4 %). Potential recombinant genotypes (polymerase/capsid) were detected in 51 GII strains (15.9 %), including the most frequent type, GII.12/GII.3 (28 strains), and GII.16/GII.2, detected for the first time in China, which were found in only children. The results indicated that genetically similar noroviruses were circulating among children and adults as a cause of gastroenteritis, except for some recombinant genotypes.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant no. 81071352) and grant WG09C08 from Wuhan Hygiene Bureau to Yuan-Hong Wang and a Grant-in-Aid for Scientific Research (grant no. 22406017, to Nobumichi Kobayashi) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We are extremely grateful to the doctors who were responsible for collecting specimens in hospitals. We thank Dr. Harry Vennema for his critical explanations and comments on the genotyping of noroviruses, and Dr. Ming Tan for his suggestions on sequencing.

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Author notes

  1. Ting Yang

    Present address: Ji An NO.1 Senior High School, Ji An, 343000, Jiangxi, People’s Republic of China

Authors and Affiliations

  1. Virology Section, Wuhan Centers for Disease Prevention and Control, 24 Jianghan North Road, Wuhan, 430015, Hubei, People’s Republic of China

    Yuan-Hong Wang, Dun-Jin Zhou, Xuan Zhou, Bei-Bei Pang, Jin-Song Peng, Man-Qing Liu & Quan Hu

  2. College of Life Science, Central China Normal University, Wuhan, 430079, People’s Republic of China

    Ting Yang

  3. Department of Hygiene, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-ku, Sapporo, 060-8556, Japan

    Souvik Ghosh & Nobumichi Kobayashi

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  1. Yuan-Hong Wang
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Supplementary material 1 (XLS 45 kb)

705_2012_1437_MOESM2_ESM.ppt

Fig. S1 Incidence of norovirus (number of norovirus-positive specimens) in children and adults in each month from January 2007 to May 2010. Stool specimens were not collected from children during Jan.- May 2007 or from adults during Feb.- May 2010. (PPT 80 kb)

705_2012_1437_MOESM3_ESM.ppt

Fig.S2 Similarity plots of GII recombinant noroviruses E2116 ((a), GII16/GII.2) and Z791((b), GII12/GII.3) found in this study. Similarity plots were performed using SimPlot version 3.5 with a slide window width of 200 bp and a step size 20 bp. SimPlot analysis was conducted with 1093-bp sequences fragments including part of the RDRP and capsid gene. GenBank accession numbers from parental norovirus strains are as follows: TV24 (GII.3), U02030;; Melksham/94/UK (GII.2), X81879; Neustrelitz260/00/DE (GII.16), AY772730; SaitamaU1/JP (GII.12), AB039775; Camberwell (GII.4), AF145896. (PPT 306 kb)

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Wang, YH., Zhou, DJ., Zhou, X. et al. Molecular epidemiology of noroviruses in children and adults with acute gastroenteritis in Wuhan, China, 2007-2010. Arch Virol 157, 2417–2424 (2012). https://doi.org/10.1007/s00705-012-1437-1

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