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RNA silencing suppressor Pns11 of rice gall dwarf virus induces virus-like symptoms in transgenic rice

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Abstract

Transgenic rice (Oryza sativa) plants expressing the Pns11 protein of rice gall dwarf virus (RGDV) displayed multiple abnormal phenotypes, some of which were highly reminiscent of the symptoms observed in RGDV-infected rice. Further analysis indicated that the apparent alterations in plant growth and morphology were correlated with the expression levels of microRNA160, microRNA162, microRNA167, microRNA168, and the microRNA target OsARF8. Especially, the striking dwarfing phenotype depended on the high expression level of microRNA167. By analogy to other categories of plant viruses, the RNA silencing suppressors encoded by plant dsRNA viruses function as pathogenicity determinants. These findings significantly deepen our current mechanistic understanding of the RNA silencing suppressor (VSR) encoded by a dsRNA virus and provide additional evidence that interference with microRNA expression is a VSR function utilized by a diverse range of viruses.

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Acknowlegments

This study was supported in part by grants from the National Natural Science Foundation of China (Grant Nos. 30370929 and 30671358).

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Authors and Affiliations

  1. State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, Guangzhou, 510642, Guangdong, China

    Wen-Jin Shen, Xin-Shen Li, Qin Zhao & Hua-Ping Li

  2. Laboratory of Plant Virology, South China Agricultural University, Guangzhou, 510642, China

    Wen-Jin Shen, Xiao-Lei Ruan, Xin-Shen Li, Qin Zhao & Hua-Ping Li

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  1. Wen-Jin Shen
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  2. Xiao-Lei Ruan
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  3. Xin-Shen Li
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Correspondence to Hua-Ping Li.

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Shen, WJ., Ruan, XL., Li, XS. et al. RNA silencing suppressor Pns11 of rice gall dwarf virus induces virus-like symptoms in transgenic rice. Arch Virol 157, 1531–1539 (2012). https://doi.org/10.1007/s00705-012-1339-2

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  • DOI: https://doi.org/10.1007/s00705-012-1339-2

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