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A survey of geminiviruses and associated satellite DNAs in the cotton-growing areas of northwestern India

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Abstract

Severe symptoms of cotton leaf curl disease (CLCuD) are caused by the association of a single-stranded circular DNA satellite (betasatellite) with a helper begomovirus. In this study, we analyzed 40 leaf samples (primarily cotton with CLCuD symptoms and other plants growing close by) from four sites between New Delhi and the Pakistan/India border, using rolling-circle amplification (RCA) and PCR. In total, the complete sequences of 12 different helper viruses, eight alphasatellites, and one betasatellite from five different plant species were obtained. A recombinant helper virus molecule found in okra and a novel alphasatellite-related DNA from croton are also described. This is the first report of the presence of both DNA components (helper virus and betasatellite) associated with resistance-breaking CLCuD in India, and it highlights the need for further work to combat its damage and spread.

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Acknowledgments

Many thanks to Dr. Dilip Mongia, CICR Sirsa, Prof. Narayan Rishi, CCS Agriculture University, Hissar, for assistance in the field, and to Mirco Boem, Laura Chiappetta, Slavica Matic, Marco Morroni, Arianna Friscina and Amit Bhardwaj for technical support and sound advice. This work was funded in part by Fondazione Cassamarca. Financial support of ICGEB is acknowledged.

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Authors and Affiliations

  1. Plant Virology Group, ICGEB Biosafety Outstation, Via Piovega 23, 31056, Ca’ Tron di Roncade, Italy

    Valerio Zaffalon, Jeremy R. Thompson & Mark Tepfer

  2. Department of Environmental Agronomy and Crop Science, University of Padova, Viale dell’Università 16, 35020, Legnaro, Padova, Italy

    Valerio Zaffalon

  3. Plant Biology Group, ICGEB Campus, Aruna Asaf Ali Marg, 110 067, New Delhi, India

    Sunil Kumar Mukherjee & Vanga Siva Reddy

  4. Department of Plant Pathology and Plant-Microbe Biology, Plant Science 334, Cornell University, Ithaca, NY, 14853, USA

    Jeremy R. Thompson

  5. Institut Jean-Pierre Bourgin UMR1318, INRA-Versailles, 78026, Versailles Cedex, France

    Mark Tepfer

  6. INRA, UR407 Pathologie Végétale, 84140, Montfavet cedex, France

    Mark Tepfer

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  1. Valerio Zaffalon
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  2. Sunil Kumar Mukherjee
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  3. Vanga Siva Reddy
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  4. Jeremy R. Thompson
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  5. Mark Tepfer
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Correspondence to Jeremy R. Thompson or Mark Tepfer.

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Zaffalon, V., Mukherjee, S.K., Reddy, V.S. et al. A survey of geminiviruses and associated satellite DNAs in the cotton-growing areas of northwestern India. Arch Virol 157, 483–495 (2012). https://doi.org/10.1007/s00705-011-1201-y

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