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Characterization and transient replication of tomato leaf curl virus defective DNAs

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Summary.

Distinct subgenomic DNA species known as defective (df) DNA molecules were found in plants infected with tomato leaf curl virus (TLCV). Four df DNAs derived from TLCV Type and Darwin 1 strains were found to contain large deletions that disrupt all of the viral genes required for viral replication, encapsidation and spread. However, the viral origin of replication (ori), including the replication-associated protein (Rep) binding domains, was present in all four df DNAs. Co-agroinfection of leaf strips with tandem repeat constructs of the viral and df DNAs resulted in their replication in the presence of the respective TLCV strain. However, the df DNAs failed to move in whole plants when co-inoculated with TLCV. The df DNAs were shown to be associated with TLCV coat protein, which may indicate encapsidation. Mutational analysis showed the minimum sequence requirements for df DNA replication by TLCV to be the intergenic region containing the Rep-binding domains.

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References

  • SAA Behjatnia IB Dry LR Krake BD Conde MI Connelly JW Randles MA Rezaian (1996) ArticleTitleNew potato spindle tuber viroid and tomato leaf curl geminivirus strains from a wild Solanum sp Phytopathology 86 880–886 Occurrence Handle10.1094/Phyto-86-880 Occurrence Handle1:CAS:528:DyaK28Xltl2rs7k%3D

    Article  CAS  Google Scholar 

  • SAA Behjatnia IB Dry MA Rezaian (1998) ArticleTitleIdentification of the replication-associated protein binding domain within the intergenic region of tomato leaf curl geminivirus Nucleic Acids Res 26 925–931 Occurrence Handle10.1093/nar/26.4.925 Occurrence Handle1:CAS:528:DyaK1cXhvVWnuro%3D

    Article  CAS  Google Scholar 

  • SAA Behjatnia IB Dry MA Rezaian (2001) ArticleTitleSequence divergence in new strains of Tomato leaf curl virus resulting in replication specificity Australas Plant Pathol 30 337–342 Occurrence Handle10.1071/AP01042

    Article  Google Scholar 

  • M Bevan (1984) ArticleTitleBinary Agrobacterium vectors for plant transformation Nucleic Acids Res 12 8711–8721 Occurrence Handle6095209 Occurrence Handle10.1093/nar/12.22.8711 Occurrence Handle1:CAS:528:DyaL2MXktV2gug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • DM Bisaro (1994) Recombination in the geminiviruses: mechanisms for maintaining genome size and generating genomic diversity J Paszkowski (Eds) Homologous recombination and gene silencing in plants Kluwer Dordrecht 39–60

    Google Scholar 

  • RW Briddon S Mansoor ID Bedford MS Pinner K Saunders J Stanley Y Zafar KA Malik PG Markham (2001) ArticleTitleIdentification of DNA components required for induction of cotton leaf curl disease Virology 285 234–243 Occurrence Handle11437658 Occurrence Handle10.1006/viro.2001.0949 Occurrence Handle1:CAS:528:DC%2BD3MXkvVOrs7s%3D

    Article  PubMed  CAS  Google Scholar 

  • CG Casado GJ Ortiz E Padron SJ Bean R McKenna M Agbandje-McKenna MI Boulton (2004) ArticleTitleIsolation and characterization of subgenomic DNAs encapsidated in “single” T = 1 isometric particles of Maize streak virus Virology 323 164–171 Occurrence Handle15165828 Occurrence Handle10.1016/j.virol.2004.02.014 Occurrence Handle1:CAS:528:DC%2BD2cXksVemtbw%3D

    Article  PubMed  CAS  Google Scholar 

  • IR Choi DC Stenger (1995) ArticleTitleStrain-specific determinants of beet curly top geminivirus DNA replication Virology 206 904–912 Occurrence Handle7856103 Occurrence Handle10.1006/viro.1995.1013 Occurrence Handle1:CAS:528:DyaK2MXjs1Wkurs%3D

    Article  PubMed  CAS  Google Scholar 

  • RHA Coutts KW Buck (1987) ArticleTitleCassava latent virus specific DNAs in mosaic diseased cassava of Nigerian origin Neth J Plant Pathol 93 241–244 Occurrence Handle10.1007/BF01998253

    Article  Google Scholar 

  • H Czosnek R Ber N Navot Y Antignus S Cohen D Zamir (1989) ArticleTitleTomato yellow leaf curl virus DNA forms in the viral capsid, in infected plants and in the insect vector J Phytopathology 125 47–54

    Google Scholar 

  • IB Dry JE Rigden LR Krake PM Mullineaux MA Rezaian (1993) ArticleTitleNucleotide sequence and genome organization of tomato leaf curl geminivirus J Gen Virol 74 147–151 Occurrence Handle8423446 Occurrence Handle1:CAS:528:DyaK3sXltlalsr8%3D

    PubMed  CAS  Google Scholar 

  • IB Dry LR Krake JE Rigden MA Rezaian (1997) ArticleTitleA novel subviral agent associated with a geminivirus – the first report of a DNA satellite Proc Natl Acad Sci USA 94 7088–7093 Occurrence Handle9192696 Occurrence Handle10.1073/pnas.94.13.7088 Occurrence Handle1:CAS:528:DyaK2sXktF2qsbw%3D

    Article  PubMed  CAS  Google Scholar 

  • P Etessami J Watts J Stanley (1989) ArticleTitleSize reversion of African cassava mosaic virus coat protein gene deletion mutants during infection of Nicotiana benthamiana J Gen Virol 70 277–289 Occurrence Handle2732690 Occurrence Handle1:CAS:528:DyaL1MXhs1Kqtbs%3D

    PubMed  CAS  Google Scholar 

  • T Frischmuth J Stanley (1991) ArticleTitleAfrican cassava mosaic virus DI DNA interferes with the replication of both genomic components Virology 183 539–544 Occurrence Handle1853559 Occurrence Handle10.1016/0042-6822(91)90982-H Occurrence Handle1:CAS:528:DyaK3MXltVOhu7g%3D

    Article  PubMed  CAS  Google Scholar 

  • T Frischmuth J Stanley (1992) ArticleTitleCharacterization of beet curly top virus subgenomic DNA localizes sequences required for replication Virology 189 808–811 Occurrence Handle1641993 Occurrence Handle10.1016/0042-6822(92)90612-S Occurrence Handle1:CAS:528:DyaK38XlsVClu7o%3D

    Article  PubMed  CAS  Google Scholar 

  • T Frischmuth J Stanley (1994) ArticleTitleBeet curly top virus symptom amelioration in Nicotiana benthamiana transformed with a naturally occurring viral subgenomic DNA Virology 200 826–830 Occurrence Handle8178467 Occurrence Handle10.1006/viro.1994.1251 Occurrence Handle1:CAS:528:DyaK2cXktVais7c%3D

    Article  PubMed  CAS  Google Scholar 

  • WDO Hamilton DM Bisaro KW Buck (1982) ArticleTitleIdentification of novel DNA forms in tomato golden mosaic virus infected tissue. Evidence for a two component viral genome Nucleic Acids Res 10 4901–4912 Occurrence Handle6290994 Occurrence Handle10.1093/nar/10.16.4901 Occurrence Handle1:CAS:528:DyaL38XlsF2ksrk%3D

    Article  PubMed  CAS  Google Scholar 

  • KP Hennegan KJ Danna (1998) ArticleTitlepBIN20: an improved binary vector for shape Agrobacterium-mediated transformation Plant Mol Biol Reptr 16 129–131 Occurrence Handle10.1023/A:1007444100898 Occurrence Handle1:CAS:528:DyaK1MXjs1OrtL8%3D

    Article  CAS  Google Scholar 

  • SM Kahn W Jiang C Borner K O’Driscoll IB Weinstein (1990) ArticleTitleConstruction of defined deletion mutants by thermal cycled fusion: applications to protein kinase C Technique J meth cell mol biol 2 27–30 Occurrence Handle1:CAS:528:DyaK3cXlt1Orur0%3D

    CAS  Google Scholar 

  • B Lin SAA Behjatnia IB Dry JW Randles MA Rezaian (2003) ArticleTitleHigh-affinity Rep-binding is not required for the replication of a geminivirus DNA and its satellite Virology 305 353–363 Occurrence Handle12573580 Occurrence Handle10.1006/viro.2002.1671 Occurrence Handle1:CAS:528:DC%2BD3sXptlOrtQ%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • H MacDonald RHA Coutts KW Buck (1988) ArticleTitleCharacterization of a subgenomic DNA isolated from Triticum aestivum plants infected with wheat dwarf virus J Gen Virol 69 1339–1344 Occurrence Handle1:CAS:528:DyaL1cXktlaqt7c%3D

    CAS  Google Scholar 

  • SW MacDowell RHA Coutts KW Buck (1986) ArticleTitleMolecular characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus Nucleic Acids Res 14 7967–7984 Occurrence Handle3022243 Occurrence Handle10.1093/nar/14.20.7967 Occurrence Handle1:CAS:528:DyaL2sXhtlygsw%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • S Mansoor RW Briddon Y Zafar J Stanley (2003) ArticleTitleGeminivirus disease complexes: an emerging threat Trends Plant Sci 8 128–134 Occurrence Handle12663223 Occurrence Handle10.1016/S1360-1385(03)00007-4 Occurrence Handle1:CAS:528:DC%2BD3sXit1Kls7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • J Ndunguru JP Legg IBF Fofana TAS Aveling G Thompson CM Fauquet (2006) ArticleTitleIdentification of a defective molecule derived from DNA-A of the bipartite begomovirus of East African cassava mosaic virus Plant Pathol 55 2–10 Occurrence Handle10.1111/j.1365-3059.2005.01289.x Occurrence Handle1:CAS:528:DC%2BD28XisFWqtLk%3D

    Article  CAS  Google Scholar 

  • K Palanichelvam T Kunik V Citovsky Y Gafni (1998) ArticleTitleThe capsid protein of tomato yellow leaf curl virus binds cooperatively to single-stranded DNA J Gen Virol 79 2829–2833 Occurrence Handle9820160 Occurrence Handle1:CAS:528:DyaK1cXmvFyqt7g%3D

    PubMed  CAS  Google Scholar 

  • BL Patil I Dasgupta (2006) ArticleTitleDefective interfering DNAs of plant viruses Crit Rev Plant Sci 25 47–64 Occurrence Handle10.1080/07352680500391295 Occurrence Handle1:CAS:528:DC%2BD28Xms1yr

    Article  CAS  Google Scholar 

  • M Paximadis MEC Rey (2001) ArticleTitleGenome organization of Tobacco leaf curl Zimbabwe virus, a new, distinct monopartite begomovirus associated with subgenomic defective DNA molecules J Gen Virol 82 3091–3097 Occurrence Handle11714987 Occurrence Handle1:CAS:528:DC%2BD3MXovVCks7w%3D

    PubMed  CAS  Google Scholar 

  • CJJM Raemakers E Sofiari N Taylor G Henshaw E Jacobsen RGF Visser (1996) ArticleTitleProduction of transgenic cassava (Manihot esculenta Crantz) plants by particle bombardment using luciferase activity as selection marker Mol Breeding 2 339–349 Occurrence Handle10.1007/BF00437912 Occurrence Handle1:CAS:528:DyaK2sXht1Gltbk%3D

    Article  CAS  Google Scholar 

  • JE Rigden IB Dry LR Krake MA Rezaian (1996) ArticleTitlePlant virus DNA replication processes in Agrobacterium: insight into the origins of geminiviruses? Proc Natl Acad Sci USA 93 10280–10284 Occurrence Handle8816791 Occurrence Handle10.1073/pnas.93.19.10280 Occurrence Handle1:CAS:528:DyaK28XlslGktbs%3D

    Article  PubMed  CAS  Google Scholar 

  • EJF Roberts KW Buck RHA Coutts (1988) ArticleTitleCharacterization of potato yellow mosaic virus as a Geminivirus with a bipartite genome Intervirology 29 162–169 Occurrence Handle3182230 Occurrence Handle1:CAS:528:DyaL1cXlt1GmsLs%3D

    PubMed  CAS  Google Scholar 

  • MR Rojas AO Noueiry WJ Lucas RL Gilbertson (1998) ArticleTitleBean dwarf mosaic geminivirus movement proteins recognize DNA in a form- and size-specific manner J Gen Virol 95 105–113 Occurrence Handle1:CAS:528:DyaK1cXmslWlsrc%3D

    CAS  Google Scholar 

  • M Saeed SAA Behjatnia S Mansoor Y Zafar S Hasnain MA Rezaian (2005) ArticleTitleA single complementary-sense transcript of a geminiviral DNA β satellite is determinant of pathogenicity Mol Plant Microbe Interact 18 7–14 Occurrence Handle15672813 Occurrence Handle1:CAS:528:DC%2BD2MXmsFKh

    PubMed  CAS  Google Scholar 

  • F Sanger S Nicklen AR Coulson (1977) ArticleTitleDNA sequencing with chain-terminating inhibitors Proc Natl Acad Sci USA 74 5463–5467 Occurrence Handle271968 Occurrence Handle10.1073/pnas.74.12.5463 Occurrence Handle1:CAS:528:DyaE1cXhtlaru7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • K Saunders ID Bedford RW Briddon PG Markham SM Wong J Stanley (2000) ArticleTitleA unique virus complex causes Ageratum yellow vein disease Proc Natl Acad Sci USA 97 6890–6895 Occurrence Handle10841581 Occurrence Handle10.1073/pnas.97.12.6890 Occurrence Handle1:CAS:528:DC%2BD3cXktFahtrw%3D

    Article  PubMed  CAS  Google Scholar 

  • J Stanley (2004) ArticleTitleSubviral DNAs associated with geminivirus disease complexes Vet Microbiol 98 121–129 Occurrence Handle14741124 Occurrence Handle10.1016/j.vetmic.2003.10.005 Occurrence Handle1:CAS:528:DC%2BD2cXltVGktA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • Stanley J, Bisaro DM, Briddon RW, Brown JK, Fauquet CM, Harrison BD, Rybicki EP, Stenger DC (2005) Geminiviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy. Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier/Academic Press, London, pp 301–326

  • J Stanley K Saunders MS Pinner SM Wong (1997) ArticleTitleNovel defective interfering DNAs associated with ageratum yellow vein geminivirus infection of Ageratum conyzoides Virology 239 87–96 Occurrence Handle9426449 Occurrence Handle10.1006/viro.1997.8856 Occurrence Handle1:CAS:528:DyaK2sXnvV2gtb0%3D

    Article  PubMed  CAS  Google Scholar 

  • J Stanley R Townsend (1985) ArticleTitleCharacterization of DNA forms associated with casava latent virus infection Nucleic Acids Res 13 2189–2206 Occurrence Handle4000956 Occurrence Handle10.1093/nar/13.7.2189 Occurrence Handle1:CAS:528:DyaL2MXitVSrtrw%3D

    Article  PubMed  CAS  Google Scholar 

  • DC Stenger (1995) ArticleTitleGenotypic variability and the occurrence of less than genome-length viral DNA forms in a field population of beet curly top geminivirus Phytopathology 85 1316–1322 Occurrence Handle10.1094/Phyto-85-1316 Occurrence Handle1:CAS:528:DyaK2MXpslSgtLs%3D

    Article  CAS  Google Scholar 

  • DC Stenger MC Stevenson SG Hormuzdi DM Bisaro (1992) ArticleTitleA number of subgenomic DNAs are produced following agroinoculation of plants with beet curly top virus J Gen Virol 73 237–242 Occurrence Handle1538189 Occurrence Handle1:CAS:528:DyaK38Xht1Clu7Y%3D Occurrence Handle10.1099/0022-1317-73-2-237

    Article  PubMed  CAS  Google Scholar 

  • XR Tao XP Zhou (2004) ArticleTitleA modified viral satellite DNA that suppresses gene expression in plants Plant J 38 850–860 Occurrence Handle15144385 Occurrence Handle10.1111/j.1365-313X.2004.02087.x Occurrence Handle1:CAS:528:DC%2BD2cXltlCrs7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • JE Thomas PR Massalski BD Harrison (1986) ArticleTitleProduction of monoclonal antibodies to African cassava mosaic virus and differences in their reactivities with other whitefly-transmitted geminiviruses J Gen Virol 67 2739–2748

    Google Scholar 

  • T Wetzel T Candresse G Macquaire M Ravelonandro J Dunez (1992) ArticleTitleA highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection J Virol Methods 39 27–37 Occurrence Handle1430063 Occurrence Handle10.1016/0166-0934(92)90122-T Occurrence Handle1:CAS:528:DyaK3sXhs1Wrt70%3D

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. CSIRO Plant Industry, Glen Osmond, Australia

    S. A. A. Behjatnia, I. B. Dry & M. A. Rezaian

  2. Plant Protection Department, College of Agriculture, Shiraz University, Shiraz, Iran

    S. A. A. Behjatnia

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  1. S. A. A. Behjatnia
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  2. I. B. Dry
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  3. M. A. Rezaian
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Behjatnia, S., Dry, I. & Rezaian, M. Characterization and transient replication of tomato leaf curl virus defective DNAs. Arch Virol 152, 1127–1138 (2007). https://doi.org/10.1007/s00705-006-0929-2

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