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Molecular characterization of two sweepoviruses from China and evaluation of the infectivity of cloned SPLCV-JS in Nicotiana benthamiana

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Abstract

Sweepoviruses are important begomoviruses that infect Ipomoea plants worldwide and cause sweet potato yield losses and cultivar decline. Two sweepoviruses, sweet potato leaf curl virus-Jiangsu (SPLCV-JS) and sweet potato leaf curl China virus-Zhejiang (SPLCCNV-ZJ), were cloned from diseased sweet potato plants collected in the Jiangsu and Zhejiang provinces of China. Sequence characterization and phylogenetic analysis demonstrated that both are typical monopartite begomoviruses and have close relationships to several reported SPLCV and SPLCCNV isolates, respectively, from Asian countries. Analysis of the protein alignments and subcellular localizations of the six SPLCV-JS proteins was also conducted to verify their putative functions. In Nicotiana benthamiana, an infectivity assay of the infectious SPLCV-JS clone resulted in mild symptoms and weak viral DNA accumulation. Interestingly, SPLCV-JS, together with a heterologous betasatellite DNA (tomato yellow leaf curl China virus isolate Y10 [TYLCCNV-Y10] DNA-β), showed a synergistic effect on enhanced symptom severity and viral DNA accumulation. This is the first reported infectious SPLCV clone.

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Acknowledgments

We thank Prof. Xueping Zhou from Zhejiang University for the TYLCCNV-Y10 DNA-A, DNA-β and DNAmβ:Su clones and helpful discussions. This work was supported by grants from the National High Technology Research and Development Program (2009AA10Z102), the Ministy of Agriculture (No. 2009ZX08010-010B), the Chinese Academy of Sciences (No. KSCX2-EW-J-12), and the Shanghai Municipal Afforestation & City Appearance and Environmental Sanitation Administration (G102410).

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  1. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China

    Huiping Bi & Peng Zhang

  2. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China

    Huiping Bi & Peng Zhang

  3. Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai Chenshan Botanical Garden, 3888 Chenhua Road, Shanghai, 201602, China

    Peng Zhang

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GenBank accession numbers FJ176701 for SPLCV-JS, JF736657 for SPLCCNV-ZJ, JN657686 for DI-DNA of SPLCV-JS and JN657687 for DI-DNA of SPLCCNV-ZJ.

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Bi, H., Zhang, P. Molecular characterization of two sweepoviruses from China and evaluation of the infectivity of cloned SPLCV-JS in Nicotiana benthamiana . Arch Virol 157, 441–454 (2012). https://doi.org/10.1007/s00705-011-1194-6

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