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Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia

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Abstract

Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.

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Notes

  1. See: www.pcvd.net

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Acknowledgments

This work was funded by the Serbian Ministry of Science and Technological Development, project number TR 31062.

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Authors and Affiliations

  1. Department for Swine Diseases, Institute of Veterinary Medicine Belgrade, Vojvode Toze 14, 11 000, Belgrade, Serbia

    Bozidar Savic, Vesna Milicevic, Dobrila Jakic-Dimic, Radisa Prodanovic & Branislav Kureljusic

  2. Faculty of Veterinary Medicine, University of Belgrade, B. Oslobodjena 18, 11000, Belgrade, Serbia

    Jovan Bojkovski

  3. Department for Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Dositej Obradovic Square 8, 21000, Novi Sad, Serbia

    Aleksandar Potkonjak

  4. Clinical Center of Serbia, Visegradska 26, 11000, Belgrade, Serbia

    Borivoje Savic

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  1. Bozidar Savic
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  2. Vesna Milicevic
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Correspondence to Bozidar Savic.

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Savic, B., Milicevic, V., Jakic-Dimic, D. et al. Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia. Arch Virol 157, 21–28 (2012). https://doi.org/10.1007/s00705-011-1130-9

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  • DOI: https://doi.org/10.1007/s00705-011-1130-9

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