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P1 Protein of Cocksfoot Mottle Virus is Indispensable for the Systemic Spread of the Virus

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Abstract

Cocksfoot mottle sobemovirus (CfMV) encodes a non-conserved protein P1 from the 5′ ORF1 of genomic RNA. The functions of CfMV P1 are unknown. In the current study we show that P1-deficient CfMV can replicate both in oat leaves and barley suspension culture cells but can not infect oat plants systemically. However, the absence of P1 reduces the efficiency of virus accumulation considerably. The infectivity of the mutant virus restores as a result of the spontaneous transversion. CfMV P1:EGFP shows a very limited cell-to-cell movement in leaf epidermal cells. In Sf9 insect cells CfMV P1 localizes in the fraction of membranes and inclusions but not in soluble cytoplasmic protein fraction.

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References

  1. R. Hull, C.M. Faquet, R.C. Gregerich, S.A. Lommel, G. Thottapilly in Virus Taxonomy. The Seventh Report of the International Committee on Taxonomy of Viruses. (Academic Press, San Diego, 2000), pp. 764–768

  2. K. Mäkinen K. Mäkelainen N. Arshava T. Tamm A. Merits E. Truve S. Zavriev M. Saarma (2000) J. Gen. Virol. 81 2783–2789 Occurrence Handle11038392

    PubMed  Google Scholar 

  3. A.E. Gorbalenya E.V. Koonin V.M. Blinov A.P. Donchenko (1988) FEBS Lett. 236 287–290 Occurrence Handle3165867 Occurrence Handle1:CAS:528:DyaL1cXls1Cmtr8%3D Occurrence Handle10.1016/0014-5793(88)80039-5

    Article  PubMed  CAS  Google Scholar 

  4. K. Mäkinen V. Naess T. Tamm E. Truve A. Aaspõllu M. Saarma (1995) Virology 207 566–571 Occurrence Handle7886961 Occurrence Handle10.1006/viro.1995.1118

    Article  PubMed  Google Scholar 

  5. F. Wilk Particlevan der M. Verbeek A.M. Dullemans J.F. Heuvel Particlevan den (1998) Virology 234 300–303

    Google Scholar 

  6. D.L. Hacker K. Sivakumaran (1997) Virology 234 317–327 Occurrence Handle9268164 Occurrence Handle1:CAS:528:DyaK2sXls1Omtr0%3D Occurrence Handle10.1006/viro.1997.8667

    Article  PubMed  CAS  Google Scholar 

  7. K. Mäkinen T. Tatum V. Naess E. Truve Ü. Puurand T. Munthe M. Saarma (1995) J. Gen. Virol. 76 2817–2825 Occurrence Handle7595389

    PubMed  Google Scholar 

  8. A. Ngon M.N. Yassi C. Ritzenthaler C. Brugidou C. Fauquet R.N. Beachy (1994) J. Gen. Virol. 75 249–257 Occurrence Handle10.1099/0022-1317-75-2-249

    Article  Google Scholar 

  9. Y. Othman R. Hull (1995) Virology 206 287–297 Occurrence Handle7831784 Occurrence Handle1:CAS:528:DyaK2MXksFSkur4%3D Occurrence Handle10.1016/S0042-6822(95)80044-1

    Article  PubMed  CAS  Google Scholar 

  10. T. Tamm E. Truve (2000) J. Virol. 74 6231–6241 Occurrence Handle10864632 Occurrence Handle1:CAS:528:DC%2BD3cXks1Kmt7o%3D Occurrence Handle10.1128/JVI.74.14.6231-6241.2000

    Article  PubMed  CAS  Google Scholar 

  11. C. Bonneau C. Brugidou L. Chen R.N. Beachy C. Fauquet (1998) Virology 244 79–86 Occurrence Handle9581781 Occurrence Handle1:CAS:528:DyaK1cXivF2ju7w%3D Occurrence Handle10.1006/viro.1998.9100

    Article  PubMed  CAS  Google Scholar 

  12. O. Voinnet Y.M. Pinto D.C. Baulcombe (1999) Proc. Natl. Acad. Sci. USA 96 14147–14152 Occurrence Handle10570213 Occurrence Handle1:CAS:528:DyaK1MXns1yjs7o%3D Occurrence Handle10.1073/pnas.96.24.14147

    Article  PubMed  CAS  Google Scholar 

  13. K. Sivakumaran B.C. Fowler D.L. Hacker (1998) Virology 252 376–386 Occurrence Handle9878617 Occurrence Handle1:CAS:528:DyaK1MXjtFGjsg%3D%3D Occurrence Handle10.1006/viro.1998.9489

    Article  PubMed  CAS  Google Scholar 

  14. T. Tamm K. Mäkinen E. Truve (1999) Arch. Virol. 144 1557–1567 Occurrence Handle10486110 Occurrence Handle1:CAS:528:DyaK1MXmtl2lu78%3D Occurrence Handle10.1007/s007050050610

    Article  PubMed  CAS  Google Scholar 

  15. T. Tamm E. Truve (2000) Virus Res. 66 197–207 Occurrence Handle10725552 Occurrence Handle1:CAS:528:DC%2BD3cXhvV2lsLY%3D Occurrence Handle10.1016/S0168-1702(00)00125-8

    Article  PubMed  CAS  Google Scholar 

  16. A. Ritala A.M. Nuutila R. Aikasalo V. Kauppinen J. Tammisola (2002) Crop Sci. 42 278–285 Occurrence Handle11756286 Occurrence Handle1:CAS:528:DC%2BD38XmsVantbg%3D Occurrence Handle10.2135/cropsci2002.0278

    Article  PubMed  CAS  Google Scholar 

  17. A.H. Christensen P.H. Quail (1996) Transgenic Res. 5 2133–2218 Occurrence Handle10.1007/BF01969712

    Article  Google Scholar 

  18. V.C. Ciccarone, V.A. Luckow, D.A. Polayes in A Laboratory Manual vol. 1: Culture and Biochemical Analysis of Cells, ed. by D.L. Spector, R.D. Goldman, L.A. Leinwald. (Cold Spring Harbor Laboratory Press, NY, 1998), pp.66.1–66.28

  19. D. Hockenbery G. Nunez C. Milliman R.D. Schreiber S.J. Korsmeyer (1990) Nature 348 334–336 Occurrence Handle2250705 Occurrence Handle1:CAS:528:DyaK3MXit1ehuw%3D%3D Occurrence Handle10.1038/348334a0

    Article  PubMed  CAS  Google Scholar 

  20. E. Schreiber P. Matthias M.M. Muller W. Schaffner (1989) Nucleic Acids Res. 17 6419 Occurrence Handle2771659 Occurrence Handle1:CAS:528:DyaL1MXltlShtr8%3D

    PubMed  CAS  Google Scholar 

  21. C. Brugidou C. Holt A. Ngon M. Yassi S. Zhang R. Beachy C. Fauquet (1995) Virology 206 108–115 Occurrence Handle7831766 Occurrence Handle1:CAS:528:DyaK2MXksFSktL8%3D Occurrence Handle10.1016/S0042-6822(95)80025-5

    Article  PubMed  CAS  Google Scholar 

  22. M.A. Mayo, W.A. Miller in The luteoviridae, ed. by G.H. Smith, H. Barker, (CABI, Wallingford, Conn, 1999), pp. 32–42

  23. F. Wilk Particlevan der P. Houterman J. Molthoff F. Hans B. Dekker J. Heuvel Particlevan den H. Huttinga R. Goldbach (1997) Mol. Plant Microbe Interact. 10 153–159 Occurrence Handle9057321

    PubMed  Google Scholar 

  24. E. Sadowy A. Maasen M. Juszczuk C. David W. Zagorski-Ostoja B. Gronenborn M.D. Hulanicka (2001) J. Gen. Virol. 82 1529–1532 Occurrence Handle11369900 Occurrence Handle1:CAS:528:DC%2BD3MXkt1Cht7Y%3D

    PubMed  CAS  Google Scholar 

  25. V. Ziegler-Graff V. Brault T. Mutterer T. Simonis E. Herrbach H. Guilley K.E. Richards G. Jonard (1996) Mol. Plant Microbe Interact. 9 501–510 Occurrence Handle1:CAS:528:DyaK28XkslOqt74%3D

    CAS  Google Scholar 

  26. S. Pfeffer P. Dunoyer F. Heim K.E. Richards G. Jonard V. Ziegler-Graff (2002) J. Virol. 76 6815–6824 Occurrence Handle12050394 Occurrence Handle1:CAS:528:DC%2BD38XkvVGitLk%3D Occurrence Handle10.1128/JVI.76.13.6815-6824.2002

    Article  PubMed  CAS  Google Scholar 

  27. G. Moissiard O. Voinnet (2004) Mol. Plant Pathol. 5 71–82 Occurrence Handle1:CAS:528:DC%2BD2cXitVKgsb4%3D Occurrence Handle10.1111/j.1364-3703.2004.00207.x Occurrence Handle20565584

    Article  CAS  PubMed  Google Scholar 

  28. D. Silhavy J. Burgyan (2004) Trends Plant Sci. 9 76–83 Occurrence Handle15102373 Occurrence Handle1:CAS:528:DC%2BD2cXhtVOrsr4%3D Occurrence Handle10.1016/j.tplants.2003.12.010

    Article  PubMed  CAS  Google Scholar 

  29. E. Waigmann S. Ueki K. Trutnyeva V. Citovsky (2004) Crit. Rev. Plant Sci. 23 195–250 Occurrence Handle1:CAS:528:DC%2BD2cXlvFWrtL0%3D Occurrence Handle10.1080/07352680490452807

    Article  CAS  Google Scholar 

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Acknowledgements

Barley (Hordeum vulgare, cv. Pokko) cell culture (VTT-G-93001) was obtained form VTT Biotechnology, Plant Biotechnology Group. We thank Cecilia Sarmiento for critical reading of the manuscript. The study was supported by Estonian Science Foundation grants 5909 and 5939.

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Authors and Affiliations

  1. Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 19086, Tallinn, Estonia

    Merike Meier, Heiti Paves, Allan Olspert & Erkki Truve

  2. National Institute of Chemical Physics and Biophysics, Akadeemia tee 23, 12618, Tallinn, Estonia

    Merike Meier, Heiti Paves, Allan Olspert, Tiina Tamm & Erkki Truve

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  1. Merike Meier
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  2. Heiti Paves
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  3. Allan Olspert
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  4. Tiina Tamm
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  5. Erkki Truve
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Correspondence to Merike Meier.

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Meier, M., Paves, H., Olspert, A. et al. P1 Protein of Cocksfoot Mottle Virus is Indispensable for the Systemic Spread of the Virus. Virus Genes 32, 321–326 (2006). https://doi.org/10.1007/s11262-005-6917-x

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  • DOI: https://doi.org/10.1007/s11262-005-6917-x

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