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First isolation and genetic characteristics of porcine sapeloviruses in Hunan, China

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Abstract

Outbreaks of diarrhea in piglets cause serious economic consequences in China. Diarrhetic fecal samples from 20 Hunan farm piglets were tested and found to be positive for porcine epidemic diarrhea virus (PEDV) by RT-PCR, although incubation with porcine kidney (PK-15) cells failed to produce infectious PEDV. Four porcine sapelovirus (PSV) strains (designated as PSV-HuNs) were isolated from four of the samples. Genomic sequence analysis revealed open reading frames encoding polyproteins of 2,331 (HuN1, 2 and 3) and 2,332 (HuN4) amino acids. Homology comparisons of the VP1 gene of the four Hunan strains with previously reported PSV strains revealed nucleotide sequence identities ranging from 74.2 to 98.6%, and deduced amino acid sequence identities from 79.5 to 98%. Phylogenetic analyses based on full-length and partial VP1 gene sequences showed that 3 of the PSV-HuN strains (HuN2, 3 and 4) clustered within a clade distinct from HuN1 as well as from all PSVs previously isolated in China, thereby showing that genetic diversity exists within Chinese PSVs. In addition, recombination analysis among PSVs indicates that a recombinant (HuN2 strain) exist in China.

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Acknowledgements

We thanks Dr. JB Campbell for the helpful comments on the manuscript.

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Authors and Affiliations

  1. College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Taotao Yang, Runcheng Li, Wang Peng, Meng Ge, Binyu Luo, Tailong Qu & Xinglong Yu

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  1. Taotao Yang
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  2. Runcheng Li
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Correspondence to Xinglong Yu.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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705_2017_3264_MOESM1_ESM.pdf

Supplementary Fig. 1 Phylogenetic trees based on VP4 gene sequence (a), VP2 gene sequence (b), VP3 gene sequence (c), 2A gene sequences (d), 2B gene sequences (e), 2C gene sequences (f), 3A gene sequences (g), 3B gene sequences (h), 3C gene sequences (i) and 3D gene sequences (j) of 12 PSV strains. These trees was constructed using the neighbor-joining method with 1,000 bootstrap replicates, using MEGA version 5.1 with pairwise distance. The scale bar indicates nucleotide substitution per site. Simian sapelovirus and avian sapelovirus were used as the outgroups (PDF 72 kb)

Supplementary material 2 (PDF 44 kb)

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Yang, T., Li, R., Peng, W. et al. First isolation and genetic characteristics of porcine sapeloviruses in Hunan, China. Arch Virol 162, 1589–1597 (2017). https://doi.org/10.1007/s00705-017-3264-x

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  • DOI: https://doi.org/10.1007/s00705-017-3264-x

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