Skip to main content
SpringerLink
Log in

Biological and molecular characterization of Capsicum chlorosis virus infecting chilli and tomato in India

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Two isolates of Capsicum chlorosis virus (CaCV, genus Tospovirus) from tomato (CaCV-To-Ind) and chilli (CaCV-Ch-Pan), collected from Haryana and Uttar Pradesh states of northern India respectively, were compared. A comparison of the amino acid sequences of their N genes revealed more than 96% identity, confirming that the virus isolates in India have a high degree of sequence conservation and are closely related to Australian isolates. Analysis of the host range of CaCV revealed no biological difference between the isolates, but they differed from CaCV-Australia. The nucleotide sequences of S, M and L RNA of CaCV-Ch-Pan were determined. The S RNA contains 3,105 nucleotides (nt), with NSs and N genes of 1,320 and 828 nt, respectively. The M RNA consists of 4,821 nt, with an NSm gene of 927 nt and a Gn/Gc gene of 3,366 nt. The intergenic regions of S and M RNA contain 824 and 425 nt, respectively. The L RNA consists of 8,912 nt, with an RNA-dependent RNA polymerase gene of 8,634 nt.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Bucher E, Sijen T, de Haan P, Goldbach R, Prins M (2003) Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. J Virol 77:1329–1336

    Article  CAS  PubMed  Google Scholar 

  2. Bag S, Druffel KL, Salewsky T, Pappu HR (2009) Nucleotide sequence and genome organization of the medium RNA of Iris yellow spot virus from the United States. Arch Virol 154:715–718

    Article  CAS  PubMed  Google Scholar 

  3. Chiemsombat P, Gajanandana O, Warin N, Hongprayoon R, Bhunchoth A, Pongsapich P (2008) Biological and molecular characterization of tospoviruses in Thailand. Arch Virol 153:571–577

    Article  CAS  PubMed  Google Scholar 

  4. Chu FH, Chao CH, Chung MH, Chen CC, Yeh SD (2001) Completion of the genome sequence of Watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. Phytopathology 91:361–368

    Article  CAS  PubMed  Google Scholar 

  5. Ciuffo M, Tavella L, Pacifico D, Masenga V, Turina M (2008) A member of a new Tospovirus species isolated in Italy from wild buckwheat (Polygonum convolvulus). Arch Virol 153:2059–2068

    Article  CAS  PubMed  Google Scholar 

  6. de Haan P, de Avila AC, Kormelink R, Westerbroek A, Gielen JL, Peters D, Goldbach RW (1992) The nucleotide sequence of the S RNA of impatiens necrotic spot virus, a novel tospovirus. FEBS Lett 306:27–32

    Article  PubMed  Google Scholar 

  7. de Haan P, Kormelink R, Resende RO, van Poelwijk F, Peters D, Goldbach R (1991) Tomato spotted wilt virus L RNA encodes a putative RNA-polymerase. J Gen Virol 72:2207–2216

    Article  PubMed  Google Scholar 

  8. de Haan P, Wagemakers L, Peters D, Goldbach R (1990) The S RNA segment of Tomato spotted wilt virus has an ambisense character. J Gen Virol 71:1001–1007

    Article  PubMed  Google Scholar 

  9. Elliot RM (1990) Molecular biology of the Bynyaviridae. J Gen Virol 71:501–522

    Article  Google Scholar 

  10. Francki RIB, Fauquet CM, Knudson DL, Brown F (1991) Classification and nomenclature of viruses. Arch Virol 2:281–283

    Google Scholar 

  11. German TL, Ullman DE, Moyer JW (1992) Tospoviruses: diagnosis, molecular biology, phylogeny and vector relationships. Ann Rev Phytopathol 30:315–348

    Article  CAS  Google Scholar 

  12. Hassani-Mehraban A, Saaijer J, Peters D, Goldbach R, Kormelink R (2006) Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept. Arch Virol 152:85–96

    Article  PubMed  Google Scholar 

  13. Hassani-Mehraban A, Saaijer J, Peters D, Goldbach R, Kormelink R (2005) A new tomato-infecting tospovirus from Iran. Phytopathology 95:852–858

    Article  CAS  PubMed  Google Scholar 

  14. Jain RK, Pappu HR, Pappu SS, Krishnareddy M, Vani A (1998) Watermelon bud necrosis tospovirus from India is a distinct virus species belonging to serogroup IV. Arch Virol 43:1637–1644

    Article  Google Scholar 

  15. Jones RAC, Sharman M (2005) Capsicum chlorosis virus infecting Capsicum annuum in East Kimberley region of Western Australia. Aust Plant Pathol 34:397–399

    Article  CAS  Google Scholar 

  16. Knierim D, Blawid R, Maiss E (2006) The complete nucleotide sequence of a Capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand. Arch Virol 151:1761–1782

    Article  CAS  PubMed  Google Scholar 

  17. Kormelink R, de Haan P, Meurs C, Peters D, Goldbach R (1992) The nucleotide sequence of the M RNA segment of Tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments. J Gen Virol 73:2795–2804

    Article  CAS  PubMed  Google Scholar 

  18. Kormelink R, Storms M, van Lent J, Peters D, Goldbach R (1994) Expression and subcellular location of the NSm protein of Tomato spotted wilt virus (TSWV), a putative viral movement protein. Virology 200:56–65

    Article  CAS  PubMed  Google Scholar 

  19. Krishnareddy M, Usha Rani R, AnilKumar KS, MadhaviReddy K, Pappu HR (2008) Capsicum chlorosis virus (Genus Tospovirus) Infecting Chili Pepper (Capsicum annuum) in India. Plant Dis 92:1469

    Article  Google Scholar 

  20. Kunkalikar S, Sudarsana P, Rajagopalan P, Zehr UB, Naidu RA, Ravi KS (2007) First report of Capsicum chlorosis virus in tomato in India. Plant Health Progress. doi:10.1094/PHP-2007-1204-01-BR

  21. McMichael LA, Persley DM, Thomas JE (2002) A new tospovirus serogroup IV species infecting capsicum and tomato in Queensland, Australia. Aust Plant Pathol 31:231–239

    Article  Google Scholar 

  22. Moyer JW (1999) Tospoviruses (Bunyaviridae). In: Granoff A, Webster RG (eds) Encyclopedia of virology. Academic Press, New York, pp 1803–1807

    Google Scholar 

  23. Okuda M, Taba S, Hanada K (2003) The S RNA segment determines symptom differences on Tetragonia expansa between two Watermelon silver mottle virus isolates. Physiol Mol Plant Pathol 62:327–332

    Article  CAS  Google Scholar 

  24. Pappu SS, Bhat AI, Pappu HR, Deom CM, Culbreath AK (2000) Phylogenetic studies of tospoviruses (Family: Bunyaviridae) based on intergenic region sequences of small and medium genomic RNAs. Arch Virol 145:1035–1045

    Article  CAS  PubMed  Google Scholar 

  25. Persley DM, Thomas JE, Sharman M (2006) Tospovirus-an Australian perspective. Aust Plant Pathol 35:161–180

    Article  Google Scholar 

  26. Polston JE, McGovern RJ, Brown LG (1999) Introduction of Tomato yellow leaf curl virus in Florida and implications for the spread of this and other geminiviruses of tomato. Plant Dis 83:984–988

    Article  Google Scholar 

  27. Premachandra WTSD, Borgemeister C, Maiss E, Knierim D, Poehling HM (2005) Ceratothripoides claratris, a new vector of a Capsicum chlorosis virus isolate infecting tomato in Thailand. Phytopathology 95:659–663

    Article  CAS  PubMed  Google Scholar 

  28. Qui WP, Geske SM, Hickey CM, Moyer JW (1998) Tomato spotted wilt tospovirus genome reassortment and genome segment-specific adaptation. Virology 244:186–194

    Article  Google Scholar 

  29. Ravi KS, Kitkaru AS, Winter S (2006) Iris yellow spot virus in onions: a new tospovirus record from India. Plant Pathol 55:288

    Article  Google Scholar 

  30. Reddy DVR, Ratna AS, Sudarshana MR, Poul F, Kiran Kumar I (1992) Serological relationships and purification of Peanut bud necrosis virus, a tospovirus occuring in peanut (Arachis hypogaea L.) in India. Ann Appl Biol 120:279–286

    Article  Google Scholar 

  31. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  32. Saritha RK, Jain RK (2007) Nucleotide sequence of the S and M RNA segments of a Groundnut bud necrosis virus isolate from Vigna radiata in India. Arch Virol 152:1195–1200

    Article  CAS  PubMed  Google Scholar 

  33. Satyanarayana T, Gowda S, Reddy LK, Mitchell SE, Dawson WO, Reddy DVR (1998) Peanut yellow spot virus is a member of a new serogroup of Tospovirus genus based on small (S) RNA sequence and organization. Arch Virol 143:353–364

    Article  CAS  PubMed  Google Scholar 

  34. Satyanarayana T, Mitchell SE, Reddy DVR, Brown S, Kresovich S, Jarret R, Naidu RA, Demski JW (1996) Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and identity to other tospoviruses. Arch Virol 141:85–98

    Article  CAS  PubMed  Google Scholar 

  35. Takeda A, Sugiyama K, Nagano H, Mori M, Kaido M, Mise K, Tsuda S, Okuno T (2002) Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Lett 532:75–79

    Article  CAS  PubMed  Google Scholar 

  36. van Knippenberg I, Goldbach R, Kormelink R (2005) Tomato spotted wilt virus S-segment mRNAs have overlapping 3′ ends containing a predicted stem-loop structure and conserved sequence motif. Virol Res 110:125–131

    Article  Google Scholar 

  37. Varma A (2007) Emergence and re emergence of plant viruses in India: impact and management options. Tenth plant virus epidemeology symposium, Hyderabad, India

  38. Williams LV, López Lambertini PM, Shohara K, Biderbost EB (2001) Occurrence and geographical distribution of tospovirus species infecting tomato crops. Argent Plant Dis 85:1227–1229

    Article  Google Scholar 

  39. Yeh SD, Chang TF (1995) Nucleotide sequence of the N gene of Watermelon silver mottle virus, a proposed pew member of the genus Tospovirus. Phytopathology 85:58–64

    Article  CAS  Google Scholar 

  40. Yeh SD, Sun IJ, Ho HM, Chang TF (1996) Molecular cloning and sequence analysis of the S RNA of Watermelon silver mottle virus. Acta Horti 431:244–259

    CAS  Google Scholar 

Download references

Acknowledgments

We are thankful to Dr. Shyi Dong Yeh, NCHU, Taiwan, for kindly providing the PAbs against the CaCV N protein and MAbs to the WSMoV-N and NSs proteins. We also thank Dr. Stephen Winter (DSMZ, Braunchewig, Germany) for providing PAbs to the CaCV N protein.

Author information

Authors and Affiliations

  1. Mahyco Research Center, Post Box No. 76, Dawalwadi, Jalna, Maharashtra, 431 203, India

    S. R. Kunkalikar, P. Sudarsana, P. Rajagopalan, Usha B. Zehr & K. S. Ravi

Authors
  1. S. R. Kunkalikar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. Sudarsana
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. Rajagopalan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Usha B. Zehr
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. S. Ravi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. S. Ravi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kunkalikar, S.R., Sudarsana, P., Rajagopalan, P. et al. Biological and molecular characterization of Capsicum chlorosis virus infecting chilli and tomato in India. Arch Virol 155, 1047–1057 (2010). https://doi.org/10.1007/s00705-010-0681-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-010-0681-5

Keywords

Search

Navigation