Abstract
Twenty-eight isolates of tospoviruses associated with tomato, pepper, cucurbits, peanut, and Physalis plants collected from fields in different regions of Thailand were characterized. On the basis of N gene and protein sequence relationships, three tospoviruses were identified, namely Watermelon silver mottle virus (WSMoV), Capsicum chlorosis virus (CaCV), and Melon yellow spot virus (MYSV). CLUSTAL analysis of selected N protein sequences showed different isolates of CaCV in three distinct clades. Based on necrosis symptoms on tomato and their 93% identity to CaCV isolates in the other two clades, CaCV-TD8, CaCV-AIT and CaCV-KS16-Thailand tomato tospovirus were designated as CaCV-tomato necrosis strain. A phylogenetic tree based on the 413-amino-acid Gc fragment of the CaCV-Pkk isolate supported the existence of three distinct CaCV clades. Vigna unguiculata produced concentric rings useful for discriminating the Thai CaCV peanut isolates from tomato or pepper isolates. By using reverse transcription polymerase chain reaction with species-specific primers, the three tospoviruses could be detected in mixed infections in watermelon and Physalis, as well as in the bodies of thrips vectors, Thrips palmi and Scirtothrips dorsalis, collected from fields.
References
Adkins S (2000) Tomato spotted wilt virus—positive steps towards negative success. Mol Plant Pathol 1:151–157
Bhunchote A, Chiemsombat P, Seepiban C, Wongyam S, Hongprayoon R, Gajanandana O (2005) Molecular characterization of Melon yellow spot virus infecting cucurbits in Thailand. BioThailand 2005 National Conference organized by National Center for Genetic Engineering and Biotechnology. November 2–5, 2005, Bangkok Thailand (Abstract in English)
Cenis JL, Perez P, Fereres A (1993) Identification of aphid (Homoptera, Aphididae) species and clones by random amplified polymorphic DNA. Ann Entomol Soc Am 86:545–550
Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116
Chen KR, Xu ZY, Yan LY (2006) Complete sequence analysis of genomic S RNA of Capsicum chlorosis virus infecting peanuts in China. Virol Sin 21:506 (Abstract in English)
Cortes I, Saaijer J, Wongjkaew S, Pereira A-M, Goldbach R, Peters D, Kormelink R (2001) Identification and characterization of a novel tospovirus species using a new RT-PCR approach. Arch Virol 146:265–278
Cortez I, Aires A, Pereira AM, Goldbach R, Kormelink R (2002) Genetic organisation of iris yellow spot virus M RNA: indications for functional homology between the G(C) glycoproteins of tospoviruses and animal-infecting bunyaviruses. Arch Virol 147:2313–2325
De Avila AC, de Haan P, Kormelink R, Resende R deO, Goldbach RW, Peters D (1993) Classification of tospoviruses based on the phylogeny of nucleocapsid gene sequences. J Gen Virol 74:153–159
Dewey RA, Semorile LC, Crici JV, Grau O (1996) Clustering of Argetinean tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene. Virus Genes 13:255–262
Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy: eight report of the international committe on taxonomy of viruses. Academic, New York, p 1162
Hassani-Mehraban A, Saaijer J, Peters D, Goldbach R, Kormelink R (2006) Molecular and biological comparison of twoTomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept. Arch Virol 152:85–96
Hsu H-T, Ueng PP, Chu F-H, Ye Z, Yeh S-D (2000) Serological and molecular characterization of a high temperature-recovered virus belonging to tospovirus serogroup IV. J Plant Pathol 66:167–175
Kato K, Hanada K, Kameya-Iwaki M (2000) Melon yellow spot virus: a distinct species of the genus Tospovirus isolated from melon. Phytopathology 90:422–426
Knierim D, Blawid R, Maiss E (2006) The complete nucleotide sequence of a capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand. Arch Virol 151:1761–1782
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163
McMichael LA, Persley DM, Thomas JE (2002) A new tospovirus serogroup IV species infecting capsicum and tomato in Queensland, Australia. Aust Plant Pathol 31:231–239
Moyer JW (1999) Tospoviruses (Bunyaviridae). In: Granoff A, Webster RG (eds) Encyclopedia of virology. Academic, San Diego, CA, pp 1803–1807
Nagata T, Carvalho KR, Noronha EF, Sodre RDA, Dutra LS, Oliveira PA, Lovato FA, Resende RO, De Avila AC, Inouya-Nagata AK (2007) The glycoprotein gene of Chrysanthemum stem necrosis virus and Zucchini lethal chlorosis virus and molecular relationship with other tospoviruses. Virus Genes 35:785–793
Okuda M, Kato K, Hanada K, Iwanami T (2006) Nucleotide sequence of melon yellow spot virus M RNA segment and characterization of non-viral sequences in subgenomic RNA. Arch Virol 151:1–11
Pappu SS, Bhat AI, Pappu HR, Deom CM, Culbreath AK (2000) Phylogenetic studies of tospoviruses (Family Bunyaviridae) based on intergenic region sequence of small and medium genomic RNAs. Arch Virol 145:1035–1045
Persley DM (2003) Capsicum chlorosis virus. In: Pernezny K, Roberts PD, Murphy JF, Goldberg NP (eds) Compendium of pepper diseases, p 27. American Phytopathological Society, St. Paul, MN
Premachandra WTSD, Borgemeister C, Maiss E, Knierim D, Poehling H-M (2005) Ceratothripoides claratris, a new vector of a capsicum chlorosis virus isolate infecting tomato in Thailand. Phytopathology 95:659–663
Pongsapich P, Chiemsombat P (2002) Characterization of tospovirus infecting tomatoes in Thailand revealed the presence of serogroup IV-tospovirus but not serogroup I-tomato spotted wilt virus. In: Proceedings of the 1st International Conference on Tropical and Subtropical Plant Diseases Nov. 5–8, 2002, Chiang Mai Thailand (Summary, p 92)
Reddy DVR, Ratna AS, Sudarshana MR, Poul F, Kumar IK (1992) Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L) in India. Ann Appl Biol 120:279–286
Satyanarayana T, Gowda S, Reddy KL, Mitchell SE, Dawson WO, Reddy DVR (1998) Peanut yellow spot virus is a member of serogroup V of tospovirus genus based on small (S) RNA sequence and organization. Arch Virol 143:353–364
Sherwood JL, German TL, Moyer JW, Ullman DE, Whitfield AE (2000) Tomato spotted wilt. In: Maloy OC, Murray TD (eds) Encyclopedia of plant pathology. Wiley, New York, pp 1030–1031
Ullman DE, Meideros R, Campbell LR, Whitfield AE, Sherwood JL, German TL (2002) Thrips as vectors of Tospoviruses. Adv Bot Res 36:113–140
Whitfield AE, Ullman DE, German TL (2005) Tospovirus-thrips interactions. Anu Rev Phytopathol 43:459–489
Wongkaew S (2002) Tospoviruses: a new emerging important virus in Asia. In: Proceedings of the 1st Interational Conference on Tropical and Subtropical Plant Diseases. Nov.5–8, 2002 Chiangmai Thailand (Summary, p 49)
Yeh S-D, Chang T-F (1995) Nucleotide sequence of the N gene of watermelon silver mottle virus, a proposed new member of the genus Tospovirus. Phytopathology 85:58–64
Acknowledgments
We thank Dr. Scott Adkins for assistance in preparing and critical reading of the manuscript. We thank Dr. John Thomas for critical reading and comments. The study was supported in part by grants from Thailand Toray Science Foundation, and from BIOTEC, Ministry of Science and Technology. Support was also provided by Center of Excellence on Agricultural Biotechnology, Kasetsart University, Kamphaengsaen Campus, Thailand.
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Chiemsombat, P., Gajanandana, O., Warin, N. et al. Biological and molecular characterization of tospoviruses in Thailand. Arch Virol 153, 571–577 (2008). https://doi.org/10.1007/s00705-007-0024-3
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DOI: https://doi.org/10.1007/s00705-007-0024-3