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Characterization of full-length VP4 genes of OP354-like P[8] human rotavirus strains detected in Bangladesh representing a novel P[8] subtype

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Abstract

The G1 and G9 rotavirus strains MMC71 and MMC38 (subgroup II, NSP4 genogroup B), respectively, isolated from children in Bangladesh, were analyzed genetically. Full-length VP4 genes of these strains had 98.9% identity to each other and showed 83.9–89.4% identity to those of the P[4] and P[8] rotaviruses. Phylogenetic analysis of VP4 nucleotide sequences revealed that strains MMC38 and MMC71 were located in a lineage of P[8] strains. However, the cluster was highly divergent from the previously established P[8] strains. The VP8* portions of strains MMC38 and MMC71 showed more than 93.9% nucleotide sequence identity to OP354-like P[8] strains, and these strains were clustered into the same lineage. These findings indicate that the VP4 of these strains should be classified into a subtype of the P[8] genotype (P[8]b) that is distinct from that of common P[8] rotaviruses (P[8]a).

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Acknowledgments

This study was supported in part by a Grant-in-Aid for Scientific Research (No. 18406018), the Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases (Okayama University, National Institute of Cholera and Enteric Diseases, India) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and a Grant for International Health Cooperation Research from the Ministry of Health, Labour, and Welfare of Japan.

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Authors and Affiliations

  1. Department of Hygiene, Sapporo Medical University School of Medicine, S-1 W-17 Chuo-ku, Sapporo, Hokkaido, 060-8556, Japan

    Shigeo Nagashima & Nobumichi Kobayashi

  2. Department of Microbiology, Mymensingh Medical College, Mymensingh, Bangladesh

    Shyamal Kumar Paul

  3. Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh

    Mohammed Mahbub Alam

  4. Division of Virology, National Institute of Cholera and Enteric Diseases, Kolkata, India

    Mamta Chawla-Sarkar & Triveni Krishnan

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  1. Shigeo Nagashima
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  2. Nobumichi Kobayashi
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  3. Shyamal Kumar Paul
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  4. Mohammed Mahbub Alam
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  5. Mamta Chawla-Sarkar
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  6. Triveni Krishnan
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Correspondence to Shigeo Nagashima.

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Nagashima, S., Kobayashi, N., Paul, S.K. et al. Characterization of full-length VP4 genes of OP354-like P[8] human rotavirus strains detected in Bangladesh representing a novel P[8] subtype. Arch Virol 154, 1223–1231 (2009). https://doi.org/10.1007/s00705-009-0436-3

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