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Genetic study of hepatitis B virus in Indonesia reveals a new subgenotype of genotype B in east Nusa Tenggara

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An Erratum to this article was published on 09 July 2008

Abstract

The hepatitis B virus (HBV) genotype is associated with viral anthropological history, clinical outcome of disease and response to treatment. This study examines the HBV genotypes in Indonesia. HBV genotypes were determined by whole-genome sequencing and from the sequence of the Pre-S2 and S regions in a larger series. Two HBV genotypes, B (HBV/B) and C (HBV/C), were predominant. Three previously reported HBV/B subgenotypes were identified, with certain population association: HBV/B2 (HBV/Ba) was found mostly in Indonesians of Chinese ethnic origin, HBV/B3 was dominant among the Javanese, and HBV/B5, reported earlier from the Philippines, was also discovered, albeit at low frequency. Two other subgenotypes, HBV/B4 from Vietnam and HBV/B6, recently reported from the Arctic region, were not found. A novel subgenotype, HBV/B7, was recognized, associated with populations of the Nusa Tenggara islands in eastern Indonesia. Characteristic differences in HBsAg serotype and single nucleotide polymorphisms (SNPs) in the Pre-S2 region distinguish HBV/B7 from other HBV/B subgenotypes and further establish the new HBV subgenotype.

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Acknowledgments

This study was partly supported by a grant from PT Bio Farma under a cooperation agreement 00713/Dir/II/1999 and No. 30/EIJK/II/1999, 11 February 1999. We thank Dr. Makoto Mayumi and Dr. Hiroaki Okamoto (Jichi Medical University, Japan) for useful discussions.

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Authors and Affiliations

  1. Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, Jakarta, 10430, Indonesia

    Neni Nurainy, David H. Muljono, Herawati Sudoyo & Sangkot Marzuki

  2. Department of Research and Development, PT Bio Farma, Jl Pasteur No. 28, Bandung, 40161, Indonesia

    Neni Nurainy

Authors
  1. Neni Nurainy
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  2. David H. Muljono
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  3. Herawati Sudoyo
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  4. Sangkot Marzuki
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Corresponding author

Correspondence to David H. Muljono.

Additional information

The nucleotide sequence data in this paper have been deposited in the GenBank database under accession Nos. EF473971–EF473977 for the whole genome of seven isolates from the Javanese (2059Java, 1839Java), Alorese and Sumba (Alr49, KD48), and Chinese (EIH45AChinese, EIH21AChinese, 2061Chinese) populations of Indonesia, and DQ141615–DQ141668 for the Pre-S2 region of 47 isolates from the three groups of ethnic populations: 16 Javanese, 16 Alorese, East Sumbanese and Kodi of the Nusa Tenggara islands, and 15 Indonesians of Chinese ethnic origin.

An erratum to this article can be found at http://dx.doi.org/10.1007/s00705-008-0142-6

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Nurainy, N., Muljono, D.H., Sudoyo, H. et al. Genetic study of hepatitis B virus in Indonesia reveals a new subgenotype of genotype B in east Nusa Tenggara. Arch Virol 153, 1057–1065 (2008). https://doi.org/10.1007/s00705-008-0092-z

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  • DOI: https://doi.org/10.1007/s00705-008-0092-z

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