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Susceptibility and immune responses following experimental infection of MHC compatible Atlantic salmon (Salmo salar L.) with different infectious salmon anaemia virus isolates

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Summary.

Infectious salmon anaemia virus (ISAV) is an aquatic orthomyxovirus causing a multisystemic disease in farmed Atlantic salmon (Salmo salar) where disease development, clinical signs, and histopathology vary to a large extent. Here, an experimental trial was designed to determine the effect of variation in viral genes on virus-host interactions, as measured by disease susceptibility and immune responses. The fish were infected using cohabitant transmission, representing a natural route of infection. Variation caused by host factors was minimized using MHC compatible A. salmon half-siblings as experimental fish. Virus isolates were selected according to HE genotype, as European ISAV isolates can be genotyped according to deletion patterns in their hemagglutinin-esterase (HE) surface glycoprotein, and the course of disease they typically induce, classified as acute versus protracted. The different ISAV isolates induced large variations in death prevalence, ranging from 0–47% in the test-group and 3–75% in the cohabitant fish. The use of MHC compatible experimental fish made it possible to determine the relative contribution of humoral versus cellular response in protection against ISA. Ability to induce a strong proliferative response correlated with survival and virus clearance, while induction of a humoral response was less protective.

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Authors and Affiliations

  1. Department of Food Safety and Infection Biology, Norwegian School of Veterinary Science, Oslo, Norway

    S. Mjaaland & T. Markussen

  2. Department of Basic Science and Aquaculture, Norwegian School of Veterinary Science, Oslo, Norway

    S. Kjøglum & U. Grimholt

  3. National Veterinary Institute, Oslo, Norway

    H. Sindre & B. H. Dannevig

  4. Department of Production Animal Medicine, Norwegian School of Veterinary Science, Epidemiological Section, Oslo, Norway

    S. Larsen

  5. Aqua Gen AS, Kyrkseterøra, Norway

    S. Kjøglum

Authors
  1. S. Mjaaland
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  2. T. Markussen
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  4. S. Kjøglum
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  5. B. H. Dannevig
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  6. S. Larsen
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  7. U. Grimholt
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Mjaaland, S., Markussen, T., Sindre, H. et al. Susceptibility and immune responses following experimental infection of MHC compatible Atlantic salmon (Salmo salar L.) with different infectious salmon anaemia virus isolates. Arch Virol 150, 2195–2216 (2005). https://doi.org/10.1007/s00705-005-0588-8

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  • DOI: https://doi.org/10.1007/s00705-005-0588-8

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