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Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses

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Summary.

Monoclonal antibody E5/G6 recognized a linear epitope common to hantavirus nucleocapsid proteins. Using synthetic peptides, we identified epitope E5/G6 as the 9 mer YEDVNGIRK (NP 165–173), in which D167, G170, I171, and R172 are indispensable. Furthermore, all the peptides synthesized using various hantavirus sequences bound MAb E5/G6 consistently, despite the existence of several amino acid variations in this region. These results indicate that MAb E5/G6 is a useful tool for detecting hantavirus antigen in rodent or patient tissues using Western blotting or other immunohistochemical assays.

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Authors and Affiliations

  1. Institute for Animal Experimentation, Graduate School of Medicine, Hokkaido University, Sapporo, Japan

    M. Okumura, K. Yoshimatsu, B.-H. Lee & J. Arikawa

  2. Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan

    K. Araki

  3. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan

    A. Asano & T. Agui

Authors
  1. M. Okumura
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  2. K. Yoshimatsu
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  3. K. Araki
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  4. B.-H. Lee
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  5. A. Asano
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  6. T. Agui
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  7. J. Arikawa
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Okumura, M., Yoshimatsu, K., Araki, K. et al. Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses. Arch Virol 149, 2427–2434 (2004). https://doi.org/10.1007/s00705-004-0393-9

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  • DOI: https://doi.org/10.1007/s00705-004-0393-9

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