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(−)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury

  • Basic Neurosciences, Genetics and Immunology - Original Article
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Abstract

A major component of green tea is (−)-epigallocatechin gallate (EGCG), which has strong antioxidant properties. Here, we investigated the effect of EGCG on neural stem cell (NSC) proliferation around the damaged area following traumatic brain injury (TBI). In this study, male Wistar rats that had access to normal drinking water, or water containing 0.1% (w/v) EGCG, ad libitum received TBI at 10 weeks of age. Immunohistochemistry revealed that the number of nestin-positive cells around the damaged area after TBI in the EGCG treatment group increased significantly compared with the normal water group (P < 0.05). However, the number of 8-hydroxy-2′-deoxyguanosine-, 4-hydroxy-2-nonenal-, single-stranded DNA (ssDNA)-positive cells and the level of peroxidation around the damaged area after TBI significantly decreased in the EGCG treatment group when compared with the water group (P < 0.05). Furthermore, in contrast to the EGCG group, almost all ssDNA-positive cells in the water group co-localized with NeuN and nestin-staining. Ex vivo studies revealed that spheres could only be isolated from injured brain tissue in the water group at 3 days following TBI. However, in the EGCG group, spheres could be isolated at both 3 and 7 days following TBI. A greater number of spheres could be isolated from the EGCG group, which differentiated into neurons and glia in culture without basic fibroblast growth factor. These results indicate that consumption of water containing EGCG pre- and post-TBI inhibits free radical-induced degradation of NSCs, which have the potential to differentiate into neurons and glia around the area of damage following TBI.

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Acknowledgments

This work was supported by the Grant-in-Aid for Scientific Research (21500803). The authors thank Mari Yachi for technical assistance.

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. Department of Pathology, Kinki University Faculty of Medicine, 377-2 Ohno-higashi, Osakasayama, Osaka, 589-8511, Japan

    Tatsuki Itoh, Akihiko Ito & Takao Satou

  2. Department of Surgery, Kinki University School of Medicine, Osaka-Sayama, Osaka, Japan

    Motohiro Imano

  3. Kinki University Faculty of Pharmaceutical Sciences, Osaka, Japan

    Shozo Nishida & Masahiro Tsubaki

  4. Life Science Research Institute, Kinki University, Osaka, Japan

    Nobuyuki Mizuguchi

  5. Division of Hospital, PL Hospital, Osaka, Japan

    Shigeo Hashimoto

  6. Division of Hospital Pathology, Hospital of Kinki University Faculty of Medicine, Osaka, Japan

    Takao Satou

Authors
  1. Tatsuki Itoh
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  2. Motohiro Imano
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  4. Masahiro Tsubaki
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  8. Takao Satou
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Correspondence to Tatsuki Itoh.

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Itoh, T., Imano, M., Nishida, S. et al. (−)-Epigallocatechin-3-gallate increases the number of neural stem cells around the damaged area after rat traumatic brain injury. J Neural Transm 119, 877–890 (2012). https://doi.org/10.1007/s00702-011-0764-9

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  • DOI: https://doi.org/10.1007/s00702-011-0764-9

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