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Cultured Rat Astrocytes Give Rise to Neural Stem Cells

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Abstract

Previously, we reported the occurrence of neural stem cells (NSCs) around an area of damage after rat traumatic brain injury (TBI), but it was unclear if this was due to blastgenesis in astrocytes, or to NSCs migrating from the subventricular zone (SVZ). In this study, NSCs were isolated and cultured from cultured type 1 astrocytes taken from newborn rat cortex in which the subventricular zone and hippocampus had been discarded. All cultured type 1 astrocytes showed glial fibrillary acidic protein (GFAP) immunopositivity. Nestin immunopositive spheres were isolated from type 1 astrocytes and cultured in the presence of bFGF and EGF in the medium. Neurospheres differentiated into Tuj1-, GFAP- and A2B5-positive cells after 4 days of culture without bFGF and EGF. These results indicate that isolated neurospheres from brain cortex astrocytes can differentiate into neurons and glia and might contribute to neurogenesis and neuroplasticity.

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References

  1. Chirumamilla S, Sun D, Bullock MR, Colello RJ (2002) Traumatic brain injury induced cell proliferation in the adult mammalian central nervous system. J Neurotrauma 19:693–703

    Article  PubMed  CAS  Google Scholar 

  2. Rice AC, Khaldi A, Harvey HB, Salman NJ, White F, Fillmore H, Bullock MR (2003) Proliferation and neuronal differentiation of mitotically active cells following traumatic brain injury. Exp Neurol 183:406–417

    Article  PubMed  CAS  Google Scholar 

  3. Azbill RD, Mu X, Bruce-Keller AJ, Mattson MP, Springer JE (1997) Impaired mitochondrial function, oxidative stress and altered antioxidant enzyme activities following traumatic spinal cord injury. Brain Res 765:283–290

    Article  PubMed  CAS  Google Scholar 

  4. Kawamata T, Katayama Y, Hovda DA, Yoshino A, Becker DP (1995) Lactate accumulation following concussive brain injury: the role of ionic fluxes induced by excitatory amino acids. Brain Res 674:196–204

    Article  PubMed  CAS  Google Scholar 

  5. Xiong Y, Gu Q, Peterson PL, Muizelaar JP, Lee CP (1997) Mitochondrial dysfunction and calcium perturbation induced by traumatic brain injury. J Neurotrauma 14:23–34

    Article  PubMed  CAS  Google Scholar 

  6. Gage FH (2000) Mammalian neural stem cells. Science 287:1433–1438

    Article  PubMed  CAS  Google Scholar 

  7. McKay R (1997) Stem cells in the central nervous system. Science 276:66–71

    Article  PubMed  CAS  Google Scholar 

  8. Lois C, Alvarez-Buylla A (1994) Long-distance neuronal migration in the adult mammalian brain. Science 264:1145–1148

    Article  PubMed  CAS  Google Scholar 

  9. Kernie SG, Erwin TM, Parada LF (2001) Brain remodeling due to neuronal and astrocytic proliferation after controlled cortical injury in mice. J Neurosci Res 66:317–326

    Article  PubMed  CAS  Google Scholar 

  10. Dash PK, Mach SA, Moore AN (2001) Enhanced neurogenesis in the rodent hippocampus following traumatic brain injury. J Neurosci Res 63:313–319

    Article  PubMed  CAS  Google Scholar 

  11. Itoh T, Satou T, Hashimoto S, Ito H (2005) Isolation of neural stem cells from damaged rat cerebral cortex after traumatic brain injury. Neuroreport 16:1687–1691

    Article  PubMed  Google Scholar 

  12. Itoh T, Satou T, Hashimoto S, Ito H (2006) Immature and mature neurons coexist among glial scars after rat traumatic brain injury. Neurol Res in press

  13. Doetsch F, Caille I, Lim DA, Garcia-Verdugo JM, Alvarez-Buylla A (1999) Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell 97:703–716

    Article  PubMed  CAS  Google Scholar 

  14. Kondo T, Raff M (2000) Oligodendrocyte precursor cells reprogrammed to become multipotential CNS stem cells. Science 289:1754–1757

    Article  PubMed  CAS  Google Scholar 

  15. O’Malley EK, Sieber BA, Black IB, Dreyfus CF (1992) Mesencephalic type I astrocytes mediate the survival of substantia nigra dopaminergic neurons in culture. Brain Res 582:65–70

    Article  PubMed  CAS  Google Scholar 

  16. McCarthy KD, de VJ (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol 85:890–902

  17. Davies SJ, Goucher DR, Doller C, Silver J (1999) Robust regeneration of adult sensory axons in degenerating white matter of the adult rat spinal cord. J Neurosci 19:5810–5822

    PubMed  CAS  Google Scholar 

  18. Jurynec MJ, Riley CP, Gupta DK, Nguyen TD, McKeon RJ, Buck CR (2003) TIGR is upregulated in the chronic glial scar in response to central nervous system injury and inhibits neurite outgrowth. Mol Cell Neurosci 23:69–80

    Article  PubMed  CAS  Google Scholar 

  19. Simpson PB, Holtzclaw LA, Langley DB, Russell JT (1998) Characterization of ryanodine receptors in oligodendrocytes, type 2 astrocytes, and O-2A progenitors. J Neurosci Res 52:468–482

    Article  PubMed  CAS  Google Scholar 

  20. Wilkin GP, Marriott DR, Cholewinski AJ (1990) Astrocyte heterogeneity. Trends Neurosci 13:43–46

    Article  PubMed  CAS  Google Scholar 

  21. Gomez-Pinilla F, Vu L, Cotman CW (1995) Regulation of astrocyte proliferation by FGF-2 and heparan sulfate in vivo. J Neurosci 15:2021–2029

    PubMed  CAS  Google Scholar 

  22. Yazaki N, Hosoi Y, Kawabata K, Miyake A, Minami M, Satoh M, Ohta M, Kawasaki T, Itoh N (1994) Differential expression patterns of mRNAs for members of the fibroblast growth factor receptor family, FGFR-1-FGFR-4, in rat brain. J Neurosci Res 37:445–452

    Article  PubMed  CAS  Google Scholar 

  23. Picard-Riera N, Nait-Oumesmar B, Baron-Van EA (2004) Endogenous adult neural stem cells: limits and potential to repair the injured central nervous system. J Neurosci Res 76:223–231

    Article  PubMed  CAS  Google Scholar 

  24. Seri B, Garcia-Verdugo JM, McEwen BS, Alvarez-Buylla A (2001) Astrocytes give rise to new neurons in the adult mammalian hippocampus. J Neurosci 21:7153–7160

    PubMed  CAS  Google Scholar 

  25. Yamamoto S, Yamamoto N, Kitamura T, Nakamura K, Nakafuku M (2001) Proliferation of parenchymal neural progenitors in response to injury in the adult rat spinal cord. Exp Neurol 172:115–127

    Article  PubMed  CAS  Google Scholar 

  26. Zhu G, Mehler MF, Mabie PC, Kessler JA (1999) Developmental changes in progenitor cell responsiveness to cytokines. J Neurosci Res 56:131–145

    Article  PubMed  CAS  Google Scholar 

  27. Xu Y, Kimura K, Matsumoto N, Ide C (2003) Isolation of neural stem cells from the forebrain of deceased early postnatal and adult rats with protracted post-mortem intervals. J Neurosci Res 74:533–540

    Article  PubMed  CAS  Google Scholar 

  28. Sergent-Tanguy S, Michel DC, Neveu I, Naveilhan P (2006) Long-lasting coexpression of nestin and glial fibrillary acidic protein in primary cultures of astroglial cells with a major participation of nestin(+)/GFAP(-) cells in cell proliferation. J Neurosci Res 83:1515–1524

    Article  PubMed  CAS  Google Scholar 

  29. Roussa E, Krieglstein K (2004) GDNF promotes neuronal differentiation and dopaminergic development of mouse mesencephalic neurospheres. Neurosci Lett 361:52–55

    Article  PubMed  CAS  Google Scholar 

  30. Bithell A, Williams BP (2005) Neural stem cells and cell replacement therapy: making the right cells. Clin Sci (Lond) 108:13–22

    Article  CAS  Google Scholar 

  31. Morizane A, Takahashi J, Shinoyama M, Ideguchi M, Takagi Y, Fukuda H, Koyanagi M, Sasai Y, Hashimoto N (2006) Generation of graftable dopaminergic neuron progenitors from mouse ES cells by a combination of coculture and neurosphere methods. J Neurosci Res 83:1015–1027

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Kinki University School of Medicine, Osaka, Japan

    Tatsuki Itoh, Takao Satou & Hiroyuki Ito

  2. Division of Hospital Pathology, Hospital of Kinki University School of Medicine, Osaka, Japan

    Takao Satou

  3. Division of Sports Medicine, Institute of Life Science, Kinki University, Osaka, Japan

    Takao Satou

  4. Kinki University School of Pharmaceutical Sciences, Osaka, Japan

    Shozo Nishida

  5. Department of Pathology, PL Hospital, Osaka, Japan

    Shigeo Hashimoto

Authors
  1. Tatsuki Itoh
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  2. Takao Satou
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  3. Shozo Nishida
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  4. Shigeo Hashimoto
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  5. Hiroyuki Ito
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Corresponding author

Correspondence to Tatsuki Itoh.

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Itoh, T., Satou, T., Nishida, S. et al. Cultured Rat Astrocytes Give Rise to Neural Stem Cells. Neurochem Res 31, 1381–1387 (2006). https://doi.org/10.1007/s11064-006-9186-8

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  • DOI: https://doi.org/10.1007/s11064-006-9186-8

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