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Non-coding RNA as a trigger of neuropathologic disorder phenotypes in transgenic Drosophila

  • Basic Neurosciences, Genetics and Immunology - Original Article
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Abstract

At most, many protein-misfolding diseases develop as environmentally induced sporadic disorders. Recent studies indicate that the dynamic interplay between a wide repertoire of noncoding RNAs and the environment play an important role in brain development and pathogenesis of brain disorders. To elucidate this new issue, novel animal models which reproduce the most prominent disease manifestations are required. For this, transgenic Drosophila strains were constructed to express small highly structured, non-coding RNA under control of a heat shock promoter. Expression of the RNA induced formation of intracellular aggregates revealed by Thioflafin T in embryonic cell culture and Congo Red in the brain of transgenic flies. Also, this strongly perturbed the brain control of locomotion monitored by the parameters of sound production and memory retention of young 5-day-old males. This novel model demonstrates that expression of non-coding RNA alone is sufficient to trigger neuropathology.

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Acknowledgments

The study was supported by Q-RNA Firm 799 (USA) and Grants of Russian Foundation for Fundamental Research 800 (E. S–P, A. P, M.E, O.Z) and Grant Molecular and Cellular Biology to M.E.

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, 199034, St Petersburg, Russia

    Elena Savvateeva-Popova, Ekaterina Nikitina, Anna Medvedeva, Dmitry Molotkov & Nicholas Kamyshev

  2. Sechenov Institute of Evolutionary Physiology and Biochemistry, 194223, St Petersburg, Russia

    Andrej Popov

  3. MNESIS Company LLC, Pleasantville, NY, 10570, USA

    Abraham Grossman

  4. Division of Biology, California Institute of Technology, Passadena, CA, 91125, USA

    Konstantin Pyatkov

  5. Engelhardt Institute of Molecular Biology, Vavilov Str. 32, 119991, Moscow, Russia

    Olga Zatsepina, Natalya Schostak, Elena Zelentsova & Michail Evgen`ev

  6. Institute of Gene Biology, Vavilov Str. 34, 119991, Moscow, Russia

    Galina Pavlova & Dmitry Panteleev

  7. Institute of Clinical Neurochemistry and National Parkinson Foundation Centre of Excellence Laboratories, Clinic and Policlinic for Psychiatry and Psychotherapy, University of Würzburg, Würzburg, Germany

    Peter Riederer

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  1. Elena Savvateeva-Popova
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  2. Andrej Popov
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  3. Abraham Grossman
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Correspondence to Elena Savvateeva-Popova.

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Savvateeva-Popova, E., Popov, A., Grossman, A. et al. Non-coding RNA as a trigger of neuropathologic disorder phenotypes in transgenic Drosophila . J Neural Transm 115, 1629–1642 (2008). https://doi.org/10.1007/s00702-008-0078-8

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