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Proteomics of the human brain: sub-proteomes might hold the key to handle brain complexity

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Summary.

Proteomics is a promising approach, which provides information about the expression of proteins and increasingly finds application in life science and disease research. Meanwhile, proteomics has proven to be applicable even on post mortem human brain tissue and has opened a new area in neuroproteomics. Thereby, neuroproteomics is usually employed to generate large protein profiles of brain tissue, which mostly reflect the expression of highly abundant proteins. As a complementary approach, the focus on sub-proteomes would enhance more specific insight into brain function. Sub-proteomes are accessible via several strategies, including affinity pull-down approaches, immunoprecipitation or subcellular fractionation. The extraordinary potential of subcellular proteomics to reveal even minute differences in the protein constitution of related cellular organelles is exemplified by a recent global description of neuromelanin granules from the human brain, which could be identified as pigmented lysosome-related organelles.

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References

  • R Aebersold DR Goodlett (2001) ArticleTitleMass spectrometry in proteomics Chem Rev 101 269–295 Occurrence Handle11712248 Occurrence Handle1:CAS:528:DC%2BD3MXltlGlug%3D%3D

    PubMed  CAS  Google Scholar 

  • R Aebersold M Mann (2003) ArticleTitleMass spectrometry-based proteomics Nature 422 198–207 Occurrence Handle12634793 Occurrence Handle1:CAS:528:DC%2BD3sXhvFKgs7s%3D

    PubMed  CAS  Google Scholar 

  • RD Bagshaw DJ Mahuran JW Callahan (2005) ArticleTitleA proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle Mol Cell Proteomics 4 133–143 Occurrence Handle15579476 Occurrence Handle1:CAS:528:DC%2BD2MXhvFKnt7g%3D

    PubMed  CAS  Google Scholar 

  • V Basrur F Yang T Kushimoto Y Higashimoto K Yasumoto J Valencia J Muller WD Vieira H Watabe J Shabanowitz VJ Hearing DF Hunt E Appella (2003) ArticleTitleProteomic analysis of early melanosomes: identification of novel melanosomal proteins J Proteome Res 2 69–79 Occurrence Handle12643545 Occurrence Handle1:CAS:528:DC%2BD38XosVKktrk%3D

    PubMed  CAS  Google Scholar 

  • M Basso S Giraudo D Corpillo B Bergamasco L Lopiano M Fasano (2004) ArticleTitleProteome analysis of human substantia nigra in Parkinson’s disease Proteomics 4 3943–3952 Occurrence Handle15526345 Occurrence Handle1:CAS:528:DC%2BD2cXhtFarsb7F

    PubMed  CAS  Google Scholar 

  • D Ben-Shachar P Riederer MB Youdim (1991) ArticleTitleIron-melanin interaction and lipid peroxidation: implications for Parkinson’s disease J Neurochem 57 1609–1614 Occurrence Handle1919577 Occurrence Handle1:CAS:528:DyaK3MXmslKqtbY%3D

    PubMed  CAS  Google Scholar 

  • SB Berman TG Hastings (1999) ArticleTitleDopamine oxidation alters mitochondrial respiration and induces permeability transition in brain mitochondria: implications for Parkinson’s disease J Neurochem 73 1127–1137 Occurrence Handle10461904 Occurrence Handle1:CAS:528:DyaK1MXlsVOitL4%3D

    PubMed  CAS  Google Scholar 

  • PA Binz DF Hochstrasser RD Appel (2003) ArticleTitleMass spectrometry-based proteomics: current status and potential use in clinical chemistry Clin Chem Lab Med 41 1540–1551 Occurrence Handle14708878 Occurrence Handle1:CAS:528:DC%2BD2cXkt1Km

    PubMed  CAS  Google Scholar 

  • A Björklund O Lindvall (1984) Dopamine-containing systems in the CNS. Handbook of chemical neuroanatomy A Björklund T Hökfelt (Eds) Classical Transmitters in the CNS, Part I NumberInSeries2 Elsevier Science Publishers Amsterdam 55–122

    Google Scholar 

  • H Braak E Braak (1986) ArticleTitleNuclear configuration and neuronal types of the nucleus niger in the brain of the human adult Hum Neurobiol 5 71–82 Occurrence Handle2426228 Occurrence Handle1:STN:280:DyaL283nslCqsw%3D%3D

    PubMed  CAS  Google Scholar 

  • C Buesa T Maes F Subirada M Barrachina I Ferrer (2004) ArticleTitleDNA chip technology in brain banks: confronting a degrading world J Neuropathol Exp Neurol 63 1003–1014 Occurrence Handle15535128 Occurrence Handle1:CAS:528:DC%2BD2cXpslCrurY%3D

    PubMed  CAS  Google Scholar 

  • DA Butterfield A Castegna (2003) ArticleTitleProteomic analysis of oxidatively modified proteins in Alzheimer’s disease brain: insights into neurodegeneration Cell Mol Biol (Noisy-le-grand) 49 747–751 Occurrence Handle1:CAS:528:DC%2BD3sXnslKlur4%3D

    CAS  Google Scholar 

  • A Castegna V Thongboonkerd JB Klein B Lynn WR Markesbery DA Butterfield (2003) ArticleTitleProteomic identification of nitrated proteins in Alzheimer’s disease brain J Neurochem 85 1394–1401 Occurrence Handle12787059 Occurrence Handle1:CAS:528:DC%2BD3sXkslOkt7k%3D

    PubMed  CAS  Google Scholar 

  • G Chambers L Lawrie P Cash GI Murray (2000) ArticleTitleProteomics: a new approach to the study of disease J Pathol 192 280–288 Occurrence Handle11054709 Occurrence Handle1:STN:280:DC%2BD3M7hsVGqtA%3D%3D

    PubMed  CAS  Google Scholar 

  • J Choudhary SG Grant (2004) ArticleTitleProteomics in postgenomic neuroscience: the end of the beginning Nat Neurosci 7 440–445 Occurrence Handle15114355 Occurrence Handle1:CAS:528:DC%2BD2cXjsFKltr0%3D

    PubMed  CAS  Google Scholar 

  • HD Coughenour RS Spaulding CM Thompson (2004) ArticleTitleThe synaptic vesicle proteome: a comparative study in membrane protein identification Proteomics 4 3141–3155 Occurrence Handle15378707 Occurrence Handle1:CAS:528:DC%2BD2cXovVynt7s%3D

    PubMed  CAS  Google Scholar 

  • C D’Ambrosio S Arena G Fulcoli MH Scheinfeld D Zhou L D’Adamio A Scaloni (2006) ArticleTitleHyperphosphorylation of JNK-interacting protein 1, a protein associated with Alzheimer disease Mol Cell Proteomics 5 97–113 Occurrence Handle16195223 Occurrence Handle1:CAS:528:DC%2BD28Xms1artA%3D%3D

    PubMed  CAS  Google Scholar 

  • P Davidsson A Brinkmalm G Karlsson R Persson M Lindbjer M Puchades S Folkesson L Paulson A Dahl L Rymo J Silberring R Ekman K Blennow (2003) ArticleTitleClinical mass spectrometry in neuroscience. Proteomics and peptidomics Cell Mol Biol (Noisy-le-grand) 49 681–688 Occurrence Handle1:CAS:528:DC%2BD3sXnslKltbo%3D

    CAS  Google Scholar 

  • DT Dexter FR Wells F Agid Y Agid AJ Lees P Jenner CD Marsden (1987) ArticleTitleIncreased nigral iron content in postmortem parkinsonian brain Lancet 2 1219–1220 Occurrence Handle2890848 Occurrence Handle1:STN:280:DyaL1c%2FmsVKgtA%3D%3D

    PubMed  CAS  Google Scholar 

  • PR Dodd JW Hambley RF Cowburn JA Hardy (1988) ArticleTitleA comparison of methodologies for the study of functional transmitter neurochemistry in human brain J Neurochem 50 1333–1345 Occurrence Handle2896227 Occurrence Handle1:CAS:528:DyaL1cXhvFynt78%3D

    PubMed  CAS  Google Scholar 

  • PF Edgar JE Douglas GJ Cooper B Dean R Kydd RL Faull (2000) ArticleTitleComparative proteome analysis of the hippocampus implicates chromosome 6q in schizophrenia Mol Psychiatry 5 85–90 Occurrence Handle10673773 Occurrence Handle1:CAS:528:DC%2BD3cXlslarsLk%3D

    PubMed  CAS  Google Scholar 

  • PF Edgar JE Douglas C Knight GJ Cooper RL Faull R Kydd (1999a) ArticleTitleProteome map of the human hippocampus Hippocampus 9 644–650 Occurrence Handle1:CAS:528:DC%2BD3cXmtFektg%3D%3D

    CAS  Google Scholar 

  • PF Edgar SJ Schonberger B Dean RL Faull R Kydd GJ Cooper (1999b) ArticleTitleA comparative proteome analysis of hippocampal tissue from schizophrenic and Alzheimer’s disease individuals Mol Psychiatry 4 173–178 Occurrence Handle1:CAS:528:DC%2BD3cXlvVWlt74%3D

    CAS  Google Scholar 

  • CD Farr PR Gafken AD Norbeck CE Doneanu MD Stapels DF Barofsky M Minami JA Saugstad (2004) ArticleTitleProteomic analysis of native metabotropic glutamate receptor 5 protein complexes reveals novel molecular constituents J Neurochem 91 438–450 Occurrence Handle15447677 Occurrence Handle1:CAS:528:DC%2BD2cXovVOmur4%3D

    PubMed  CAS  Google Scholar 

  • Fedorow H, Halliday GM, Rickert CH, Gerlach M, Riederer P, Double KL (2005a) Evidence for specific phases in the development of human neuromelanin. Neurobiol Aging

  • H Fedorow F Tribl G Halliday M Gerlach P Riederer KL Double (2005b) ArticleTitleNeuromelanin in human dopamine neurons: comparison with peripheral melanins and relevance to Parkinson’s disease Prog Neurobiol 75 109–124 Occurrence Handle1:CAS:528:DC%2BD2MXisFKjsb8%3D

    CAS  Google Scholar 

  • M Fountoulakis (2004) ArticleTitleApplication of proteomics technologies in the investigation of the brain Mass Spectrom Rev 23 231–258 Occurrence Handle15133836 Occurrence Handle1:CAS:528:DC%2BD2cXlsl2gu7s%3D

    PubMed  CAS  Google Scholar 

  • M Fountoulakis N Cairns G Lubec (1999) ArticleTitleIncreased levels of 14-3-3 gamma and epsilon proteins in brain of patients with Alzheimer’s disease and Down syndrome J Neural Transm [Suppl] 57 323–335 Occurrence Handle1:CAS:528:DC%2BD3cXhs1GrtL4%3D

    CAS  Google Scholar 

  • M Fountoulakis R Hardmeier H Hoger G Lubec (2001) ArticleTitlePostmortem changes in the level of brain proteins Exp Neurol 167 86–94 Occurrence Handle11161596 Occurrence Handle1:CAS:528:DC%2BD3MXls1WnsA%3D%3D

    PubMed  CAS  Google Scholar 

  • J Garin R Diez S Kieffer JF Dermine S Duclos E Gagnon R Sadoul C Rondeau M Desjardins (2001) ArticleTitleThe phagosome proteome: insight into phagosome functions J Cell Biol 152 165–180 Occurrence Handle11149929 Occurrence Handle1:CAS:528:DC%2BD3MXjslyhsw%3D%3D

    PubMed  CAS  Google Scholar 

  • M Gerlach P Riederer (1996) ArticleTitleAnimal models of Parkinson’s disease: an empirical comparison with the phenomenology of the disease in man J Neural Transm 103 987–1041 Occurrence Handle9013391 Occurrence Handle1:CAS:528:DyaK28XntVKku7Y%3D

    PubMed  CAS  Google Scholar 

  • C Gomez-Santos I Ferrer AF Santidrian M Barrachina J Gil S Ambrosio (2003) ArticleTitleDopamine induces autophagic cell death and alpha-synuclein in crease in human neuroblastoma SH-SY5Y cells J Neurosci Res 73 341–350 Occurrence Handle12868068 Occurrence Handle1:CAS:528:DC%2BD3sXmt1Gjsrk%3D

    PubMed  CAS  Google Scholar 

  • J Grimm A Mueller F Hefti A Rosenthal (2004) ArticleTitleMolecular basis for catecholaminergic neuron diversity Proc Natl Acad Sci USA 101 13891–13896 Occurrence Handle15353588 Occurrence Handle1:CAS:528:DC%2BD2cXotVygtbc%3D

    PubMed  CAS  Google Scholar 

  • E Grünblatt (2004) ArticleTitleThe benefits of microarrays as tools for studying neuropsychiatric disorders Drugs Today (Barc) 40 147–156

    Google Scholar 

  • E Grünblatt S Mandel J Jacob-Hirsch S Zeligson N Amariglo G Rechavi J Li R Ravid W Roggendorf P Riederer MB Youdim (2004) ArticleTitleGene expression profiling of parkinsonian substantia nigra pars compacta; alterations in ubiquitin-proteasome, heat shock protein, iron and oxidative stress regulated proteins, cell adhesion/cellular matrix and vesicle trafficking genes J Neural Transm 111 1543–1573 Occurrence Handle15455214

    PubMed  Google Scholar 

  • SP Gygi B Rist SA Gerber F Turecek MH Gelb R Aebersold (1999a) ArticleTitleQuantitative analysis of complex protein mixtures using isotope-coded affinity tags Nat Biotechnol 17 994–999 Occurrence Handle1:CAS:528:DyaK1MXmvVelsLs%3D

    CAS  Google Scholar 

  • SP Gygi Y Rochon BR Franza R Aebersold (1999b) ArticleTitleCorrelation between protein and mRNA abundance in yeast Mol Cell Biol 19 1720–1730 Occurrence Handle1:CAS:528:DyaK1MXhsFeqtrc%3D

    CAS  Google Scholar 

  • B Herbert (1999) ArticleTitleAdvances in protein solubilisation for two-dimensional electrophoresis Electrophoresis 20 660–663 Occurrence Handle10344231 Occurrence Handle1:CAS:528:DyaK1MXjsVaksLw%3D

    PubMed  CAS  Google Scholar 

  • E Hirsch AM Graybiel YA Agid (1988) ArticleTitleMelanized dopaminergic neurons are differentially susceptible to degeneration in Parkinson’s disease Nature 334 345–348 Occurrence Handle2899295 Occurrence Handle1:CAS:528:DyaL1cXltVyms78%3D

    PubMed  CAS  Google Scholar 

  • LA Huber K Pfaller I Vietor (2003) ArticleTitleOrganelle proteomics: implications for subcellular fractionation in proteomics Circ Res 92 962–968 Occurrence Handle12750306 Occurrence Handle1:CAS:528:DC%2BD3sXjsVGqtLk%3D

    PubMed  CAS  Google Scholar 

  • MR Hynd JM Lewohl HL Scott PR Dodd (2003) ArticleTitleBiochemical and molecular studies using human autopsy brain tissue J Neurochem 85 543–562 Occurrence Handle12694381 Occurrence Handle1:CAS:528:DC%2BD3sXnslyrs7g%3D Occurrence Handle10.1046/j.1471-4159.2003.01747.x

    Article  PubMed  CAS  Google Scholar 

  • K Jellinger E Kienzl G Rumpelmair P Riederer H Stachelberger D Ben-Shachar MB Youdim (1992) ArticleTitleIron-melanin complex in substantia nigra of parkinsonian brains: an x-ray microanalysis J Neurochem 59 1168–1171 Occurrence Handle1494904 Occurrence Handle1:CAS:528:DyaK38Xls1yrsL8%3D

    PubMed  CAS  Google Scholar 

  • CR Jimenez FJ Stam KW Li Y Gouwenberg MP Hornshaw F De Winter J Verhaagen AB Smit (2005) ArticleTitleProteomics of the injured rat sciatic nerve reveals protein expression dynamics during regeneration Mol Cell Proteomics 4 120–132 Occurrence Handle15509515 Occurrence Handle1:CAS:528:DC%2BD2MXhvFKnt7s%3D

    PubMed  CAS  Google Scholar 

  • NL Johnston-Wilson CD Sims JP Hofmann L Anderson AD Shore EF Torrey RH Yolken (2000) ArticleTitleDisease-specific alterations in frontal cortex brain proteins in schizophrenia, bipolar disorder, and major depressive disorder. The Stanley Neuropathology Consortium Mol Psychiatry 5 142–149 Occurrence Handle10822341 Occurrence Handle1:CAS:528:DC%2BD3cXlslartrg%3D

    PubMed  CAS  Google Scholar 

  • BA Jordan BD Fernholz M Boussac C Xu G Grigorean EB Ziff TA Neubert (2004) ArticleTitleIdentification and verification of novel rodent postsynaptic density proteins Mol Cell Proteomics 3 857–871 Occurrence Handle15169875 Occurrence Handle1:CAS:528:DC%2BD2cXotlGmurc%3D

    PubMed  CAS  Google Scholar 

  • E Jung M Heller JC Sanchez DF Hochstrasser (2000) ArticleTitleProteomics meets cell biology: the establishment of subcellular proteomes Electrophoresis 21 3369–3377 Occurrence Handle11079557 Occurrence Handle1:CAS:528:DC%2BD3cXotVWgs78%3D

    PubMed  CAS  Google Scholar 

  • Kaindl AM, Sifringer M, Zabel C, Nebrich G, Wacker MA, Felderhoff-Mueser U, Endesfelder S, von der Hagen M, Stefovska V, Klose J, Ikonomidou C (2005) Acute and long-term proteome changes induced by oxidative stress in the developing brain. Cell Death Differ. [Epub ahead of print]

  • K Kanninen G Goldsteins S Auriola I Alafuzoff J Koistinaho (2004) ArticleTitleGlycosylation changes in Alzheimer’s disease as revealed by a proteomic approach Neurosci Lett 367 235–240 Occurrence Handle15331161 Occurrence Handle1:CAS:528:DC%2BD2cXntFWqt78%3D

    PubMed  CAS  Google Scholar 

  • A Kastner EC Hirsch O Lejeune F Javoy-Agid O Rascol Y Agid (1992) ArticleTitleIs the vulnerability of neurons in the substantia nigra of patients with Parkinson’s disease related to their neuromelanin content? J Neurochem 59 1080–1089 Occurrence Handle1494900 Occurrence Handle1:CAS:528:DyaK38XlsVyms7Y%3D

    PubMed  CAS  Google Scholar 

  • Kellner R, Lottspeich F, Meyer HE (1999) Microcharacterization of Proteins, Wiley-VCH

  • J Klose (1975) ArticleTitleProtein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals Humangenetik 26 231–243 Occurrence Handle1093965 Occurrence Handle1:CAS:528:DyaE28Xltl2gtro%3D

    PubMed  CAS  Google Scholar 

  • J Klose (1999a) ArticleTitleFractionated extraction of total tissue proteins from mouse and human for 2-D electrophoresis Methods Mol Biol 112 67–85 Occurrence Handle1:CAS:528:DyaK1cXotVGrsr8%3D

    CAS  Google Scholar 

  • J Klose (1999b) ArticleTitleLarge-gel 2-D electrophoresis Methods Mol Biol 112 147–172 Occurrence Handle1:CAS:528:DyaK1cXotVGrs74%3D

    CAS  Google Scholar 

  • J Klose C Nock M Herrmann K Stuhler K Marcus M Bluggel E Krause LC Schalkwyk S Rastan SD Brown K Bussow H Himmelbauer H Lehrach (2002) ArticleTitleGenetic analysis of the mouse brain proteome Nat Genet 30 385–393 Occurrence Handle11912495 Occurrence Handle1:CAS:528:DC%2BD38Xisl2lsLs%3D

    PubMed  CAS  Google Scholar 

  • MA Korolainen G Goldsteins I Alafuzoff J Koistinaho T Pirttila (2002) ArticleTitleProteomic analysis of protein oxidation in Alzheimer’s disease brain Electrophoresis 23 3428–3433 Occurrence Handle12373773 Occurrence Handle1:CAS:528:DC%2BD38Xot1Kis70%3D

    PubMed  CAS  Google Scholar 

  • K Krapfenbauer E Engidawork N Cairns M Fountoulakis G Lubec (2003) ArticleTitleAberrant expression of peroxiredoxin subtypes in neurodegenerative disorders Brain Res 967 152–160 Occurrence Handle12650976 Occurrence Handle1:CAS:528:DC%2BD3sXitV2gsbc%3D

    PubMed  CAS  Google Scholar 

  • H Langen P Berndt D Roder N Cairns G Lubec M Fountoulakis (1999) ArticleTitleTwo-dimensional map of human brain proteins Electrophoresis 20 907–916 Occurrence Handle10344266 Occurrence Handle1:CAS:528:DyaK1MXjsVakt7k%3D

    PubMed  CAS  Google Scholar 

  • MR Larsen P Roepstorff (2000) ArticleTitleMass spectrometric identification of proteins and characterization of their post-translational modifications in proteome analysis Fresenius J Anal Chem 366 677–690 Occurrence Handle11225779 Occurrence Handle1:CAS:528:DC%2BD3cXhvF2ls7w%3D

    PubMed  CAS  Google Scholar 

  • KW Li MP Hornshaw J van Minnen KH Smalla ED Gundelfinger AB Smit (2005) ArticleTitleOrganelle Proteomics of Rat Synaptic Proteins: Correlation-Profiling by Isotope-Coded Affinity Tagging in Conjunction with Liquid Chromatography-Tandem Mass Spectrometry to Reveal Post-synaptic Density Specific Proteins J Proteome Res 4 725–733 Occurrence Handle15952719 Occurrence Handle1:CAS:528:DC%2BD2MXisleqtbY%3D

    PubMed  CAS  Google Scholar 

  • G Lominadze DW Powell GC Luerman AJ Link RA Ward KR McLeish (2005) ArticleTitleProteomic Analysis of Human Neutrophil Granules Mol Cell Proteomics 4 1503–1521 Occurrence Handle15985654 Occurrence Handle1:CAS:528:DC%2BD2MXhtFeltbzJ

    PubMed  CAS  Google Scholar 

  • F Lottspeich (1999) ArticleTitleProteome analysis: a pathway to the functional analysis of proteins Angew Chem Int Ed Engl 38 2476–2492 Occurrence Handle10508327 Occurrence Handle1:CAS:528:DyaK1MXmt1ars7w%3D

    PubMed  CAS  Google Scholar 

  • A Lüking DJ Cahill S Mullner (2005) ArticleTitleProtein biochips: a new and versatile platform technology for molecular medicine Drug Discov Today 10 789–794

    Google Scholar 

  • DM Mann PO Yates (1983) ArticleTitlePossible role of neuromelanin in the pathogenesis of Parkinson’s disease Mech Ageing Dev 21 193–203 Occurrence Handle6865505 Occurrence Handle1:CAS:528:DyaL3sXkt1Smu7k%3D

    PubMed  CAS  Google Scholar 

  • ER Marcotte DM Pearson LK Srivastava (2001) ArticleTitleAnimal models of schizophrenia: a critical review J Psychiatry Neurosci 26 395–410 Occurrence Handle11762207 Occurrence Handle1:STN:280:DC%2BD38%2Fjs1Sjuw%3D%3D

    PubMed  CAS  Google Scholar 

  • ER Marcotte LK Srivastava R Quirion (2003) ArticleTitlecDNA microarray and proteomic approaches in the study of brain diseases: focus on schizophrenia and Alzheimer’s disease Pharmacol Ther 100 63–74 Occurrence Handle14550505 Occurrence Handle1:CAS:528:DC%2BD3sXnvVOgsbw%3D

    PubMed  CAS  Google Scholar 

  • K Marcus O Schmidt H Schäfer M Hamacher A Van Hall HE Meyer (2004) ArticleTitleProteomics-application to the brain Int Rev Neurobiol 61 285–311 Occurrence Handle15482819

    PubMed  Google Scholar 

  • CD Marsden (1961) ArticleTitlePigmentation in the nucleus substantiae nigrae of mammals J Anat 95 256–261 Occurrence Handle13767161 Occurrence Handle1:STN:280:DyaF3c%2FoslWmtA%3D%3D

    PubMed  CAS  Google Scholar 

  • CD Marsden (1983) ArticleTitleNeuromelanin and Parkinson’s disease J Neural Transm [Suppl] 19 121–141 Occurrence Handle1:CAS:528:DyaL2cXkslSns7c%3D

    CAS  Google Scholar 

  • DA McRitchie GM Halliday H Cartwright (1995) ArticleTitleQuantitative analysis of the variability of substantia nigra pigmented cell clusters in the human Neuroscience 68 539–551 Occurrence Handle7477964 Occurrence Handle1:CAS:528:DyaK2MXntF2jtrw%3D

    PubMed  CAS  Google Scholar 

  • HE Meyer E Hoffmann-Posorske H Korte LM Heilmeyer SuffixJr (1986) ArticleTitleSequence analysis of phosphoserine-containing peptides. Modification for picomolar sensitivity FEBS Lett 204 61–66 Occurrence Handle3091399 Occurrence Handle1:CAS:528:DyaL28Xmt1ais7o%3D

    PubMed  CAS  Google Scholar 

  • ML Mimmack J Brooking S Bahn (2004) ArticleTitleQuantitative polymerase chain reaction: validation of microarray results from postmortem brain studies Biol Psychiatry 55 337–345 Occurrence Handle14960285 Occurrence Handle1:CAS:528:DC%2BD2cXhtFWhsb0%3D

    PubMed  CAS  Google Scholar 

  • Y Mizuno S Ohta M Tanaka S Takamiya K Suzuki T Sato H Oya T Ozawa Y Kagawa (1989) ArticleTitleDeficiencies in complex I subunits of the respiratory chain in Parkinson’s disease Biochem Biophys Res Commun 163 1450–1455 Occurrence Handle2551290 Occurrence Handle1:CAS:528:DyaL1MXmtFarsLg%3D

    PubMed  CAS  Google Scholar 

  • L Mouledous S Hunt R Harcourt J Harry KL Williams HB Gutstein (2003a) ArticleTitleNavigated laser capture microdissection as an alternative to direct histological staining for proteomic analysis of brain samples Proteomics 3 610–615 Occurrence Handle1:CAS:528:DC%2BD3sXjvFGisL8%3D

    CAS  Google Scholar 

  • L Mouledous S Hunt R Harcourt JL Harry KL Williams HB Gutstein (2003b) ArticleTitleProteomic analysis of immunostained, laser-capture microdissected brain samples Electrophoresis 24 296–302 Occurrence Handle1:CAS:528:DC%2BD3sXnvFKisA%3D%3D

    CAS  Google Scholar 

  • PH O’Farrell (1975) ArticleTitleHigh resolution two-dimensional electrophoresis of proteins J Biol Chem 250 4007–4021 Occurrence Handle236308 Occurrence Handle1:CAS:528:DyaE2MXktl2ltro%3D

    PubMed  CAS  Google Scholar 

  • S-E Ong M Mann (2005) ArticleTitleMass spectrometry-based proteomics turns quantitative Nature Chem Biol 1 252–262 Occurrence Handle1:CAS:528:DC%2BD2MXhtVamtrfI

    CAS  Google Scholar 

  • SR Owens H Breithaupt (2002) ArticleTitleFrom genomes to cures–a long way to go. More than a year after the publication of the first draft of the human genome, scientists took stock of its implications for science and society EMBO Rep 3 11–14 Occurrence Handle11799052 Occurrence Handle1:CAS:528:DC%2BD38Xht1Smsbc%3D

    PubMed  CAS  Google Scholar 

  • JJ Palacino D Sagi MS Goldberg S Krauss C Motz M Wacker J Klose J Shen (2004) ArticleTitleMitochondrial dysfunction and oxidative damage in parkin-deficient mice J Biol Chem 279 18614–18622 Occurrence Handle14985362 Occurrence Handle1:CAS:528:DC%2BD2cXjsVKmsrc%3D

    PubMed  CAS  Google Scholar 

  • L Paulson R Persson G Karlsson J Silberring A Bierczynska-Krzysik R Ekman A Westman-Brinkmalm (2005) ArticleTitleProteomics and peptidomics in neuroscience. Experience of capabilities and limitations in a neurochemical laboratory J Mass Spectrom 40 202–213 Occurrence Handle15706622 Occurrence Handle1:CAS:528:DC%2BD2MXitFyhur8%3D

    PubMed  CAS  Google Scholar 

  • E Perlson KF Medzihradszky Z Darula DW Munno NI Syed AL Burlingame M Fainzilber (2004) ArticleTitleDifferential proteomics reveals multiple components in retrogradely transported axoplasm after nerve injury Mol Cell Proteomics 3 510–520 Occurrence Handle14973157 Occurrence Handle1:CAS:528:DC%2BD2cXktFChsLg%3D

    PubMed  CAS  Google Scholar 

  • TM Preuss M Caceres MC Oldham DH Geschwind (2004) ArticleTitleHuman brain evolution: insights from microarrays Nat Rev Genet 5 850–860 Occurrence Handle15520794 Occurrence Handle1:CAS:528:DC%2BD2cXptFenuro%3D

    PubMed  CAS  Google Scholar 

  • Reichmann H, Riederer P (1989) Biochemical analyses of respiratory chain enzymes in different brain regions of patients with Parkinson’s disease. BMBF Symposium “Morbus Parkinson und andere Basalganglienerkrankungen”, Bad Kissingen, Abstract 1989, S44

  • P Riederer WD Rausch B Schmidt P Kruzik C Konradi E Sofic W Danielczyk M Fischer E Ogris (1988) ArticleTitleBiochemical fundamentals of Parkinson’s disease Mt Sinai J Med 55 21–28 Occurrence Handle3279302 Occurrence Handle1:CAS:528:DyaL1cXhsVOqu7o%3D

    PubMed  CAS  Google Scholar 

  • P Riederer E Sofic WD Rausch P Kruzik MBH Youdim (1985) Dopaminforschung heute und morgen – L-Dopa in der Zukunft H Umek (Eds) L-Dopa-Substitution der Parkinson-Krankheit: Geschichte – Gegenwart – Zukunft Springer Wien New York 127–144

    Google Scholar 

  • MM Ryan SJ Huffaker MJ Webster M Wayland T Freeman S Bahn (2004) ArticleTitleApplication and optimization of microarray technologies for human postmortem brain studies Biol Psychiatry 55 329–336 Occurrence Handle14960284 Occurrence Handle1:CAS:528:DC%2BD2cXhtFWhsbw%3D

    PubMed  CAS  Google Scholar 

  • V Santoni M Molloy T Rabilloud (2000) ArticleTitleMembrane proteins and proteomics: un amour impossible? Electrophoresis 21 1054–1070 Occurrence Handle10786880 Occurrence Handle1:CAS:528:DC%2BD3cXis12kurk%3D

    PubMed  CAS  Google Scholar 

  • AH Schapira JM Cooper D Dexter P Jenner JB Clark CD Marsden (1989) ArticleTitleMitochondrial complex I deficiency in Parkinson’s disease Lancet 1 1269 Occurrence Handle2566813 Occurrence Handle1:STN:280:DyaL1M3mtVOktQ%3D%3D

    PubMed  CAS  Google Scholar 

  • B Schilling MMS Bharath RH Row J Murray MP Cusack RA Capaldi CR Freed KN Prasad JK Andersen BW Gibson (2005) ArticleTitleRapid purification and mass spectrometric characterization of mitochondrial NADH dehydrogenase (Complex I) from rodent brain and a dopaminergic neuronal cell line Mol Cell Proteomics 4 84–96 Occurrence Handle15591592 Occurrence Handle1:CAS:528:DC%2BD2MXhtleit74%3D

    PubMed  CAS  Google Scholar 

  • SJ Schonberger PF Edgar R Kydd RL Faull GJ Cooper (2001) ArticleTitleProteomic analysis of the brain in Alzheimer’s disease: molecular phenotype of a complex disease process Proteomics 1 1519–1528 Occurrence Handle11747211 Occurrence Handle1:CAS:528:DC%2BD38XksFaksg%3D%3D

    PubMed  CAS  Google Scholar 

  • M Shamoto-Nagai W Maruyama Y Akao T Osawa F Tribl M Gerlach FA Zucca L Zecca P Riederer M Naoi (2004) ArticleTitleNeuromelanin inhibits enzymatic activity of 26S proteasome in human dopaminergic SH-SY5Y cells J Neural Transm 111 1253–1265 Occurrence Handle15480837 Occurrence Handle1:CAS:528:DC%2BD2cXotFCrur0%3D

    PubMed  CAS  Google Scholar 

  • B Sitek J Luttges K Marcus G Kloppel W Schmiegel HE Meyer SA Hahn K Stuhler (2005) ArticleTitleApplication of fluorescence difference gel electrophoresis saturation labelling for the analysis of microdissected precursor lesions of pancreatic ductal adenocarcinoma Proteomics 5 2665–2679 Occurrence Handle15924292 Occurrence Handle1:CAS:528:DC%2BD2MXmtF2gtLc%3D

    PubMed  CAS  Google Scholar 

  • J Smythies (1996) ArticleTitleOn the functional of neuromelanin Proc R Soc Lond B Biol Sci 263 487–489 Occurrence Handle1:CAS:528:DyaK28XjsVeks7w%3D

    CAS  Google Scholar 

  • DG Standaert (2005) ArticleTitleApplications of laser capture microdissection in the study of neurodegenerative disease Arch Neurol 62 203–205 Occurrence Handle15710848

    PubMed  Google Scholar 

  • D Sulzer J Bogulavsky KE Larsen G Behr E Karatekin MH Kleinman N Turro D Krantz RH Edwards LA Greene L Zecca (2000) ArticleTitleNeuromelanin biosynthesis is driven by excess cytosolic catecholamines not accumulated by synaptic vesicles Proc Natl Acad Sci USA 97 11869–11874 Occurrence Handle11050221 Occurrence Handle1:CAS:528:DC%2BD3cXnvVSgsLo%3D

    PubMed  CAS  Google Scholar 

  • SW Taylor E Fahy SS Ghosh (2003) ArticleTitleGlobal organellar proteomics Trends Biotechnol 21 82–88 Occurrence Handle12573857 Occurrence Handle1:CAS:528:DC%2BD3sXptlynsA%3D%3D

    PubMed  CAS  Google Scholar 

  • F Tribl M Gerlach K Marcus E Asan T Tatschner T Arzberger HE Meyer G Bringmann P Riederer (2005) ArticleTitle“Subcellular Proteomics” of Neuromelanin Granules Isolated from the Human Brain Mol Cell Proteomics 4 945–957 Occurrence Handle15851778 Occurrence Handle1:CAS:528:DC%2BD2MXmtFKkurw%3D

    PubMed  CAS  Google Scholar 

  • JC Trinidad A Thalhammer CG Specht R Schoepfer AL Burlingame (2005) ArticleTitlePhosphorylation state of postsynaptic density proteins J Neurochem 92 1306–1316 Occurrence Handle15748150 Occurrence Handle1:CAS:528:DC%2BD2MXislegsbo%3D

    PubMed  CAS  Google Scholar 

  • M Unlu ME Morgan JS Minden (1997) ArticleTitleDifference gel electrophoresis: a single gel method for detecting changes in protein extracts Electrophoresis 18 2071–2077 Occurrence Handle9420172 Occurrence Handle1:CAS:528:DyaK2sXnvVCns70%3D

    PubMed  CAS  Google Scholar 

  • Van Deerlin VMD, Ginsberg SD, Lee VM-Y, Trojanowski JQ (2002) The use of fixed human post mortem brain tissue tu study mRNA expression in neurodegenerative diseases: application of microdissection and mRNA amplification. Microarrays for the neurosciences: an essential guide. Gregg JP (ed) Cambridge, Massachusetts, MIT press: 201–235

  • H Voshol MJ Glucksman J van Oostrum (2003) ArticleTitleProteomics in the discovery of new therapeutic targets for psychiatric disease Curr Mol Med 3 447–458 Occurrence Handle12942998 Occurrence Handle1:CAS:528:DC%2BD3sXmsFOhsb0%3D

    PubMed  CAS  Google Scholar 

  • VP Whittaker MN Sheridan (1965) ArticleTitleThe morphology and acetylcholine content of isolated cerebral cortical synaptic vesicles J Neurochem 12 363–372 Occurrence Handle14333293 Occurrence Handle1:CAS:528:DyaF2MXktFyqu7o%3D

    PubMed  CAS  Google Scholar 

  • DA Wolters MP Washburn JR Yates Suffix3rd (2001) ArticleTitleAn automated multidimensional protein identification technology for shotgun proteomics Anal Chem 73 5683–5690 Occurrence Handle11774908 Occurrence Handle1:CAS:528:DC%2BD3MXnslaqsrw%3D

    PubMed  CAS  Google Scholar 

  • CC Wu JR Yates Suffix3rd (2003) ArticleTitleThe application of mass spectrometry to membrane proteomics Nat Biotechnol 21 262–267 Occurrence Handle12610573 Occurrence Handle1:CAS:528:DC%2BD3sXhsFajsrs%3D

    PubMed  CAS  Google Scholar 

  • LR Yu TP Conrads T Uo Y Kinoshita RS Morrison DA Lucas KC Chan J Blonder HJ Issaq TD Veenstra (2004) ArticleTitleGlobal analysis of the cortical neuron proteome Mol Cell Proteomics 3 896–907 Occurrence Handle15231876 Occurrence Handle1:CAS:528:DC%2BD2cXotlGmu7w%3D

    PubMed  CAS  Google Scholar 

  • X Yuan DM Desiderio (2005) ArticleTitleProteomics analysis of human cerebrospinal fluid J Chromatogr B Analyt Technol Biomed Life Sci 815 179–189 Occurrence Handle15652808 Occurrence Handle1:CAS:528:DC%2BD2MXksVaqtg%3D%3D

    PubMed  CAS  Google Scholar 

  • C Zabel DC Chamrad J Priller B Woodman HE Meyer GP Bates J Klose (2002) ArticleTitleAlterations in the mouse and human proteome caused by Huntington’s disease Mol Cell Proteomics 1 366–375 Occurrence Handle12118078 Occurrence Handle1:CAS:528:DC%2BD38XlslWhsrg%3D

    PubMed  CAS  Google Scholar 

  • C Zabel J Klose (2004) ArticleTitleInfluence of Huntington’s disease on the human and mouse proteome Int Rev Neurobiol 61 241–283 Occurrence Handle15482818 Occurrence Handle1:CAS:528:DC%2BD2MXnvV2hsA%3D%3D Occurrence Handle10.1016/S0074-7742(04)61010-5

    Article  PubMed  CAS  Google Scholar 

  • Y Zhou G Gu DR Goodlett T Zhang C Pan TJ Montine KS Montine RH Aebersold J Zhang (2004) ArticleTitleAnalysis of alpha-synuclein-associated proteins by quantitative proteomics J Biol Chem 279 39155–39164 Occurrence Handle15234983 Occurrence Handle1:CAS:528:DC%2BD2cXnt1yjtrY%3D

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. The National Parkinson Foundation (NPF) Research Laboratories, Miami, FL, USA

    F. Tribl & P. Riederer

  2. Medizinisches Proteom-Center, Ruhr-Universität Bochum, Bochum, Germany

    K. Marcus & H. E. Meyer

  3. Institute of Organic Chemistry, Bayerische Julius-Maximilians-Universität Würzburg, Würzburg, Germany

    F. Tribl & G. Bringmann

  4. Clinical Neurochemistry, Clinic and Polyclinic for Child & Adolescent Psychiatry & Psychotherapy, Bayerische Julius-Maximilians-Universität Würzburg, Würzburg, Germany

    M. Gerlach

  5. Department of Clinical Neurochemistry, Clinic and Polyclinic for Psychiatry & Psychotherapy, Bayerische Julius-Maximilians-Universität Würzburg, Würzburg, Germany

    F. Tribl & P. Riederer

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  1. F. Tribl
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  2. K. Marcus
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  3. G. Bringmann
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  4. H. E. Meyer
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  5. M. Gerlach
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Tribl, F., Marcus, K., Bringmann, G. et al. Proteomics of the human brain: sub-proteomes might hold the key to handle brain complexity. J Neural Transm 113, 1041–1054 (2006). https://doi.org/10.1007/s00702-006-0513-7

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  • DOI: https://doi.org/10.1007/s00702-006-0513-7

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