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Analysis of potential diagnostic biomarkers in cerebrospinal fluid of idiopathic normal pressure hydrocephalus by proteomics

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Summary

Background. The pathogenesis of idiopathic normal pressure hydrocephalus (INPH) is unknown, and the syndrome of INPH remains a diagnostic and therapeutic challenge. The present study investigated the disease-specific proteins that aid in the diagnosis and treatment of INPH and thus to study their role in the disease process.

Methods. A comparative proteomic analysis was used for clinical screening of cerebrospinal fluid (CSF) proteins in 15 patients with INPH and compared with 12 normal subjects. Furthermore, enzyme linked immunosorbent assay (ELISA) was performed for comparison with CSF proteins between individual INPH patients and controls.

Results. Seven proteins and their isoforms, including leucine-rich α-2-glycoprotein (LRG), α1-antichymotrypsin, apolipoprotein D, apolipoprotein J, haptoglobin α1, serum albumin, and α-1-microglobulin/bikunin precursor showed significant changes in CSF of INPH patients compared with controls by proteomic analysis. And significant higher CSF levels of LRG in INPH patients compared with controls were found by ELISA.

Conclusions. These results indicate that there are significant differences in the expression of certain proteins in the CSF of patients with INPH and normal subjects. In particular, the CSF level assay of LRG suggests that LRG is a specific biomarker for INPH and has potential use in the diagnosis and indication for CSF shunting.

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Authors and Affiliations

  1. Department of Neurosurgery, Juntendo University School of Medicine, Tokyo, Japan

    X. Li, M. Miyajima & H. Arai

  2. Division of Proteomics and Biomolecular Science, BioMedical Research Center, Juntendo University School of Medicine, Tokyo, Japan

    R. Mineki, H. Taka & K. Murayama

  3. Juntendo University Research Institute for Diseases of Old Ages, Tokyo, Japan

    X. Li & M. Miyajima

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  1. X. Li
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  2. M. Miyajima
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  4. H. Taka
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  5. K. Murayama
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Li, X., Miyajima, M., Mineki, R. et al. Analysis of potential diagnostic biomarkers in cerebrospinal fluid of idiopathic normal pressure hydrocephalus by proteomics. Acta Neurochir (Wien) 148, 859–864 (2006). https://doi.org/10.1007/s00701-006-0787-4

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