Abstract
Ovate Family Proteins (OFPs), members of a novel transcriptional repressor family, were involved in multiple aspects of plant growth and development. Yet not much is known about grape (Vitis vinifera) OFP genes in a systematic way. Accordingly, grape OFP genes were identified and analyzed on aspects of structure, sequence similarity and expression pattern, aiming to enhance our understanding of grape OFP genes in both evolutionary and functional perspectives. Synteny analysis indicated that segmental duplication events contributed to family expansion, and several grape OFP genes arose before the divergence of grape and Arabidopsis thaliana. Phylogenetic and structure analysis provided further insight into the evolutionary conservation and divergence of grape OFP genes. Tissue-specific and flower organ expression analysis suggested their roles in both vegetative and reproductive tissue development. Expression pattern following gibberellic acid (GA3) treatment showed that grape OFP genes are GA responsive. Expression profiling of grape OFP genes during ovule development in multiple cultivars suggests their potential function in ovule development. Taken together, this is the first time grape OFP genes were studied in combination of evolutionary and functional way, which greatly adds our knowledge of grape OFP genes and provides candidate genes for future study.
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This work was supported by the Joint Funds of the National Natural Science Foundation of China (U1603234) and Chinese Universities Scientific Fund (Z109021571).
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Online Resource 1. Genbank accession numbers of grape OFP cDNA sequences
Online Resource 2. The primer sequences used for RT-PCR amplification of grape OFP genes. The specific primers were designed based on the OFP gene sequences using the Primer 5.0 software
Online Resource 3. Synteny blocks of OFP genes within the grape genome
Online Resource 4. Block identifiers of OFP genes within the grape genome
Online Resource 5. Synteny blocks of OFP genes between the grape and Arabidopsis genomes
Online Resource 6. Block identifiers of OFP genes between the grape and Arabidopsis genomes
Online Resource 7. Alignments used to produce phylogenic tree of OFPs from multiple species
Online Resource 8. RT-PCR expression analysis of grape OFP genes in different tissues. The grape Actin and EF-1α genes were used as references. For each gene, the upper bands represent amplified products from ‘Muscut’ (M) and the lower bands represent products amplified from ‘Flame Seedless’ (F)
Online Resource 9. RT-PCR expression patterns of grape OFP genes in flower organs. The grape Actin and EF-1α genes were used as references. For each gene, the upper bands represent amplified products from ‘Muscut’ (M) and the lower bands represent products amplified from ‘Flame Seedless’ (F)
Online Resource 10. RT-PCR expression patterns of grape OFP genes in ‘Kyoho’ flowers under GA3 treatment. The grape Actin and EF-1α genes were used as reference genes. For each gene, the upper bands represent amplified products from mock sample (M) and the lower bands represent products amplified from treatment sample (T)
Online Resource 11. RT-PCR expression patterns of grape OFP genes in ovules from four grape cultivars. The grape Actin and EF-1α genes were used as reference genes. Sample collecting periods of 27, 30, 33, 36, 39 and 42 days after full bloom are indicated on the graph. For each gene, the amplified bands from top to bottom represent amplified products from ovules of ‘Muscat’ (M), ‘Kyoho’ (K), ‘Flame Seedless’ (F) and ‘Kunxiang Seedless’ (Kx)
Online Resource 12. Transcriptome data of OFP genes during ovule development of seeded and seedless grape progeny
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Wang, L., Zhang, S., Zhang, X. et al. Evolutionary and expression analysis of Vitis vinifera OFP gene family. Plant Syst Evol 304, 995–1008 (2018). https://doi.org/10.1007/s00606-018-1528-x
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DOI: https://doi.org/10.1007/s00606-018-1528-x