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Population structure and genetic analysis of different utility types of mango (Mangifera indica L.) germplasm of Andhra Pradesh state of India using microsatellite markers

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Abstract

Genetic analysis of 90 mango genotypes including juicy, table, dual and pickle types from different parts of Andhra Pradesh of India was carried out employing 143 mango-specific microsatellite markers. Of the 143, 34 were new mango-specific microsatellite loci isolated in the course of the present investigation by constructing an (CA) n and (TG) n -enriched genomic library. Characterization of the 90 genotypes resulted in the detection of 301 alleles from 106 polymorphic loci with an average of 2.87 alleles per locus and polymorphism information content of 0.67. UPGMA cluster analysis grouped all the genotypes into two major groups with a genetic similarity range of 47–88 %. Grouping of the genotypes based on the utility type was observed only at sub-cluster level. Study of population structure by a model-based STRUCTURE analysis revealed the germplasm to exist in four gene pools. Overall F st of 0.11 indicated genetic differentiation between the populations to be low. Analysis of molecular variance revealed that major proportion of the variation was within the individuals (62.25 %). The molecular marker-based study of genetic diversity suggests that the germplasm studied representing the kind of variability would be a valuable genetic resource for future breeding and association mapping in search for new and novel alleles.

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Acknowledgments

Authors gratefully acknowledge the Department of Biotechnology (DBT), New Delhi, for the financial support. Thanks are due to the Institute of Biotechnology, Acharya N.G. Ranga Agricultural University, Hyderabad (India), for providing necessary laboratory facilities to carry out the research work. We are very thankful to Dr. Trushar Shah, ICRISAT, India, for the STRUCTURE analysis.

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Authors and Affiliations

  1. Institute of Biotechnology, ANGRAU, Hyderabad, India

    Malathi Surapaneni, Lakshminarayan R. Vemireddy, C. Neetasri & E. A. Siddiq

  2. Vegetable Research Station, ARI, APHU, Hyderabad, India

    Hameedunnisa Begum & B. Purushotham Reddy

  3. Laboratory of Molecular Genetics, CDFD, Hyderabad, India

    J. Nagaraju

  4. Department of Genetics, Osmania University, Hyderabad, India

    S. Y. Anwar

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  1. Malathi Surapaneni
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  2. Lakshminarayan R. Vemireddy
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  3. Hameedunnisa Begum
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Correspondence to Malathi Surapaneni.

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Surapaneni, M., Vemireddy, L.R., Begum, H. et al. Population structure and genetic analysis of different utility types of mango (Mangifera indica L.) germplasm of Andhra Pradesh state of India using microsatellite markers. Plant Syst Evol 299, 1215–1229 (2013). https://doi.org/10.1007/s00606-013-0790-1

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