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Evaluation of genetic diversity among commercial cultivars, hybrids and local mango (Mangifera indica L.) genotypes of India using cumulative RAPD and ISSR markers

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Abstract

An assessment of genetic diversity studies was undertaken to understand the level and pattern of diversity in 65 mango (Mangifera indica L.) genotypes of India including 20 commercial cultivars, 18 hybrids, 25 local genotypes and two exotic cultivars based on qualitative and quantitative fruit characters as well as RAPD and ISSR profiles. A considerable variation was observed in respect of three important qualitative characters namely table quality, fruit attractiveness and storage life of ripe fruits and potentially superior genotypes for the above traits were identified. A wide variation was noticeable regarding metabolite composition of pulp of ripe mango fruit with respect to total soluble solids, total sugar, reducing sugar, acidity, sugar:acid ratio, ascorbic acid and phenolic content. Fifteen RAPD primers yielded 27 monomorphic and 129 polymorphic bands with percent polymorphism averaging 82.7%. Of a total 70 ISSR bands generated from eight ISSR primers, 60 bands (85.71%) were found to be polymorphic. Cumulative band data from these two methods precisely arranged accessions into eight clusters which correspond well with their pedigree relationship. UPGMA dendrograms drawn using RAPD, ISSR and cumulative data showed highly similar grouping of genotypes on the basis of their parental origin. No clear-cut geographical separation was revealed among East, West, North and South Indian mango cultivars by neither of these molecular markers nor their combinations. This supports the common gene pool origin of mango as well as operation of similar selection pressure as the cultivar preferences in these areas are largely similar.

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Acknowledgments

Financial assistance received through a project grant from the Ministry of Agriculture and Cooperation, Govt. of India, New Delhi is duly acknowledged. We wish to thank Dr. Jatindra K. Nayak, Professor of English, Utkal University, Bhubaneswar (India) for going through the manuscript and making useful suggestions.

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Authors and Affiliations

  1. DNA Fingerprinting Laboratory, Biotechnology-cum-Commercial Tissue Culture Centre, Orissa University of Agriculture and Technology, Bhubaneswar, 751003, Orissa, India

    K. C. Samal, R. C. Jena & B. K. Das

  2. Department of Botany, Utkal University, Bhubaneswar, 751002, Orissa, India

    K. C. Samal, R. C. Jena, S. S. Swain & P. K. Chand

  3. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, 751004, Orissa, India

    P. K. Chand

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  1. K. C. Samal
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Correspondence to P. K. Chand.

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Samal, K.C., Jena, R.C., Swain, S.S. et al. Evaluation of genetic diversity among commercial cultivars, hybrids and local mango (Mangifera indica L.) genotypes of India using cumulative RAPD and ISSR markers. Euphytica 185, 195–213 (2012). https://doi.org/10.1007/s10681-011-0522-y

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