Abstract
In this article, we studied the pollen morphology and wall development, microsporogenesis, male gametophyte development, and anther wall structure changes during pollen development of Cardiocrinum giganteum (Wall.) Makina from the genus Cardiocrinum (Endl.) Lindl. (Liliaceae) using paraffin sections, scanning and transmission electron microscopy, and fluorescence microscopy. The results showed that C. giganteum has oval-shaped pollen with a single sulcus and reticulate ornamentation. The exine is of the semi-tectum type and can be divided into the tectum layer, columellate layer and basal layer. Meiosis in the microsporocyte is accompanied by successive cytokinesis. The mature pollen is three-celled. The anther wall prior to maturity is built by one layer of epidermis, 1–2 layers of endothecium cells, 4–5 middle layers and 2 layers of tapetum, while upon maturity it is only built by one layer of epidermis, one layer of endothecium cells and one middle layer. The tapetal cells are secretory, with two or more nuclei. Ubisch bodies originate from rough endoplasmic reticulum except a few from mitochondria.
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This work was supported by the National Natural Science Foundation of China (grant no. 30470106) and the Beijing Natural Science Foundation of China (grant no. 8102012).
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Chen, HN., Zhao, CH., Liu, XR. et al. Pollen development of Cardiocrinum giganteum (Wall.) Makina in China. Plant Syst Evol 298, 1557–1565 (2012). https://doi.org/10.1007/s00606-012-0658-9
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DOI: https://doi.org/10.1007/s00606-012-0658-9