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A study of genetic variation and evolution of Phyllostachys (Bambusoideae: Poaceae) using nuclear restriction fragment length polymorphisms

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Abstract

Phylogenetic and taxonomic difficulties are common within the woody bamboos, due to their unique life cycle, which severely limits the availability of floral characters. To addresss some of these problems, 20 species of woody bamboos in the genus Phyllostachys were analyzed using nuclear restriction fragment length polymorphisms (RFLPs). The RFLP data were used to generate genetic distances between all pairs of taxa and to examine the degree of genetic variation within and among bamboo species. The genetic distances were also used to create dendrograms of accessions and species. These trees supported the current division of the genus into two sections and provided some information on the thorny taxonomic problems in this group. We show that RFLPs can be used for species identification and the delineation of species limits.

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References

  • Blatter E (1930) The flowering of bamboos. J Bombay Nat Hist Soc 34:135–141

    Google Scholar 

  • Chao, C, Chu C (1981) New taxa and combinations of Bambusoideae in China. J Nanjing Tech Coll For Prod 3:33–44

    Google Scholar 

  • Chou C-H, Yang C-M, Sheen S-S (1984) A biochemical aspect of phylogentic study of Bambusaceae in Taiwan. I. The genus Phyllostachys. Proc Natl Acad Sci (ROC) 8:89–98

    Google Scholar 

  • Clark LG (1990) Hunting bamboos in the Andes: a botanical perspective. Am Bamboo Soc Newsl 11:2–7

    Google Scholar 

  • Clark LG, Davidse G, Ellis RP (1989) Natural hybridization in bamboos: evidence from Chusquea sect. Swallenchloa (Poaceae: Bambusoideae) Natl Geog Res 5:459–476

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Google Scholar 

  • Felsenstein J (1993) PHYLIP (Phylogeny Inference Package), version 3.5c. Distributed by the author, Department of Genetics, University of Washington, Seattle, Wash.

    Google Scholar 

  • Friar E, Kochert G (1991) Bamboo germ plasm screening with nuclear restriction fragment length polymorphisms. Theor Appl Genet 82:697–703

    Google Scholar 

  • Janzen DH (1976) Why bamboos wait so long to flower. Annu Rev Ecol Syst 7:347–391

    Google Scholar 

  • Manokaran N (1990) The state of the rattan and bamboo trade. Rattan Information Centre, Occasional Paper 7, Forest Research Institute, Kepong-Kuala Lampur, Malaysia

    Google Scholar 

  • McClure FA (1957) Bamboos of the genus Phyllostachys under cultivation in the United States, US Dept Agric Handb No. 114. Washington, D.C.

  • McCouch SR, Kochert G, Yu ZH, Yang ZY, Kush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:148–149

    Google Scholar 

  • Miller JC, Tanksley SD (1990) RFLP analysis of phylogenetic relationships and genetic variation in the genus Lycopersicon. Theor Appl Genet 80:437–448

    Google Scholar 

  • Nei M, Li W-H (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 75:3359–3362

    Google Scholar 

  • Ohrnberger D, Goerrings J (eds) (1986) The bamboos of the world. Odenthal, FRG

    Google Scholar 

  • Rohlf FJ (1992) NTSYS-pc. Numerical taxonomy and multivariate analysis system: version 1.70. Applied Biostatistics, New York

    Google Scholar 

  • Sneath PHA, Sokol RR (1973) Numerical taxonomy. WH Freeman, San Francisco

    Google Scholar 

  • Soderstrom TR (1976) Curtains for this bamboo? Pacific Hortic 37:7–14

    Google Scholar 

  • Soderstrom TR (1981) Some evolutionary trends in the Bambusoideae (Poaceae). Ann Miss Bot Gard 68:15–47

    Google Scholar 

  • Soderstrom TR, Ellis RP (1986) The position of bamboo genera and allies in a system of grass classification. In: Soderstrom TR, Hilu KW, Campbell CS, Barkworth ME (eds): Grass systematics and evolution. Smithsonian Institution Press, Washington D.C., pp 225–238

    Google Scholar 

  • Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517

    Google Scholar 

  • Swofford DL (1993) Phylogenetic analysis using parsimony (Paup) version 3.1.1. Illinois Natural History Survey, Champaigne, Ill.

    Google Scholar 

  • Wang CP, Yu ZH, Ye GH, Chu CD, Chao CS, Chen SY, Yao CY, Zhao HR (1980a) A taxonomical study of Phyllostachys, China I. Acta Phytotaxon Sin 18:15–19

    Google Scholar 

  • Wang CP, Yu ZH, Ye GH, Chu CD, Chao CS, Chen SY, Yao CY, Zhao HR (1980b) A taxonomical study of Phyllostachys, China II. Acta Phytotaxon Sin 18:168–193

    Google Scholar 

  • Wu MC-Y (1962) Classification of Bambuseae based on leaf anatomy. Bot Bull Acad Sin 3:83–107

    Google Scholar 

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Communicated by G. S. Khush

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Friar, E., Kochert, G. A study of genetic variation and evolution of Phyllostachys (Bambusoideae: Poaceae) using nuclear restriction fragment length polymorphisms. Theoret. Appl. Genetics 89, 265–270 (1994). https://doi.org/10.1007/BF00225152

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  • DOI: https://doi.org/10.1007/BF00225152

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