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Deoxynivalenol fluorescence aptasensor based on AuCu bimetallic nanoclusters and MoS2

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Abstract

Aptamers against deoxynivalenol (DON) were selected through capture-systematic evolution of ligands by exponential enrichment. Through isothermal titration calorimetry and fluorimetric assay, aptamer candidate DN-2 demonstrated good affinity to DON with Kd value of 40.36 ± 6.32 nM. Accordingly, a Forster resonance energy transfer aptasensor was fabricated by using the aptamer DN-2 combined with AuCu bimetallic nanoclusters as energy donor and MoS2 nanosheets as energy acceptor. Under the optimal conditions, the fluorescence response was utilized for DON quantitative determination ranging from 5 to 100 ng/mL with a detection limit of 1.87 ng/mL. The practical application of this method was verified in maize flour samples and demonstrated a satisfied recovery of 94.6 ~ 103.1%. The obtained aptamers and their application in DON determination provide a new tool for DON monitoring in various foodstuff.

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Funding

This work was partially supported by the National Natural Science Fund of China (NSFC 32072310, 31871721), the Guangzhou Science and Technology Project (202206010096), the Project funded by Jiangsu Province Postdoctoral Science Foundation (1701097B), and the Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Nuo Duan, Changxin Li, Mingqian Song, Kexin Ren, Zhouping Wang & Shijia Wu

  2. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China

    Nuo Duan, Changxin Li, Mingqian Song, Kexin Ren, Zhouping Wang & Shijia Wu

  3. International Joint Laboratory On Food Safety, Jiangnan University, Wuxi, 214122, China

    Zhouping Wang & Shijia Wu

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  1. Nuo Duan
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  2. Changxin Li
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  3. Mingqian Song
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  4. Kexin Ren
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  5. Zhouping Wang
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Correspondence to Shijia Wu.

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Duan, N., Li, C., Song, M. et al. Deoxynivalenol fluorescence aptasensor based on AuCu bimetallic nanoclusters and MoS2. Microchim Acta 189, 296 (2022). https://doi.org/10.1007/s00604-022-05385-4

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  • DOI: https://doi.org/10.1007/s00604-022-05385-4

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