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A “signal off” aptasensor based on NiFe2O4 NTs and Au@Pt NRs for the detection of deoxynivalenol via voltammetry

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Abstract

A “signal off” aptasensor has been developed to detect deoxynivalenol (DON). DON aptamers (Apt) were used as biological recognition elements, nickel ferrite nanotubes (NiFe2O4 NTs) are used as the base material to increase the surface area of the electrode, and the Au@Pt NRs were used as carriers for loading signal labels thionine (Thi) and complementary strand (cDNA). In the presence of DON it will be specifically captured by Apt, then the competition mechanism was triggered; the signal molecules fall off from the electrode surface, which then causes the electrode signal to decrease. NiFe2O4 NTs and Au@Pt NRs were characterized by transmission electron microscope (TEM), scanning electron micrograph (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD). The designed sensor provides a concentration range of 1 × 10−8 to 5 × 10−4 mg mL−1 and limit of detection of 3.02 × 10−9 mg mL−1. Determination of DON in corn meal samples was investigated and the recovery was 98.4 to 103.5%. The proposed aptasensor displayed good sensitivity, high specificity, and acceptable reproducibility.

Based on NiFe2O4 NTs as substrate material and Au@Pt NRs as signal label prepared DON aptasensor for the determination of DON.

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Funding

This work was financially supported by the National Natural Science Foundation of China (Grant No. 61301037), the Program for Science and Technology Innovation Talents in Universities of Henan Province (No. 20HASTIT002), the Cultivation Plan for Young Core Teachers in Universities of Henan Province (No. 2017GGJS072), and the National Engineering Laboratory for Wheat & Corn Further Processing, Henan University of Technology (No. NL2018004).

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  1. School of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Lianhua Road 100#, Zhengzhou High & New Technology Industries Development Zone, Zhengzhou, 450001, Henan Province, People’s Republic of China

    Baoshan He & Kai Wang

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  1. Baoshan He
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Correspondence to Baoshan He.

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He, B., Wang, K. A “signal off” aptasensor based on NiFe2O4 NTs and Au@Pt NRs for the detection of deoxynivalenol via voltammetry. Microchim Acta 188, 23 (2021). https://doi.org/10.1007/s00604-020-04666-0

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