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A self-correcting fluorescent assay of tyrosinase based on Fe-MIL-88B-NH2 nanozyme

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Abstract

A self-correcting fluorescent assay of tyrosinase (TYR) was developed by utilization of Fe-MIL-88B-NH2 as a peroxidase-like nanozyme and a capture probe. Fe-MIL-88B-NH2 nanozyme was selected as an electron donor, and the oxidization product (dopamine-o-quinone) acts as an energy acceptor. First, TYR catalyzes the oxidation of tyramine hydrochloride to dopamine and then to dopamine-o-quinone. Second, Fe-MIL-88B-NH2 with intrinsic peroxidase-like activity decomposes H2O2 to produce ·OH radicals, which further accelerate the oxidation of dopamine to dopamine-o-quinone. Excessive H2O2 and ·OH radicals reduce the interferences from ascorbic acid at the same time providing a self-correcting ability. Dopamine-o-quinone reacts with -NH2 groups on the ligand of Fe-MIL-88B-NH2 through Michael reaction which results in fluorescence quenching. Under 365-nm excitation, the fluorescence emission intensity at 452 nm gradually decreased with increasing TYR concentration varying from 0 to 10 U mL−1. The linear range is from 1 to 5 U mL−1 and the detection limit is 0.05679 U mL−1. This self-correcting fluorescent assay of tyrosinase exhibits good sensitivity and selectivity which is also successfully applied for tyrosinase inhibitor detection.

Graphical abstract

Schematic representation of fluorescent assay for tyrosinase determination based on Fe-MIL-88B-NH2 nanozyme.

A self-correcting fluorescent assay for tyrosinase was developed based on the Fe-MIL-88B-NH2 nanozyme.

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Funding

The authors are grateful for the financial support from the National Natural Science Foundation of China (21765002), the Natural Science Foundation of Guangxi (AD19110004, 2017GXNSFDA198044), and the BAGUI Scholar Program.

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Authors and Affiliations

  1. School of Chemistry and Pharmaceutical Science, State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, 541004, Guangxi, People’s Republic of China

    Ying Sun, Tianran Lin, Cuihong Zeng, Gaoyan Jiang, Xuanhan Zhang, Fanggui Ye & Shulin Zhao

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  1. Ying Sun
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Correspondence to Tianran Lin or Fanggui Ye.

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Sun, Y., Lin, T., Zeng, C. et al. A self-correcting fluorescent assay of tyrosinase based on Fe-MIL-88B-NH2 nanozyme. Microchim Acta 188, 158 (2021). https://doi.org/10.1007/s00604-021-04808-y

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