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Titanium dioxide–mediated resistive nanobiosensor for E. coli O157:H7

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Abstract

A titanium dioxide nanoparticle (TiO2 NP)–mediated resistive biosensor is described for the determination of DNA fragments of Escherichia coli O157:H7 (E. coli O157:H7). The sol-gel method was used to synthesize the TiO2 NP, and microlithography was applied to fabricate the interdigitated sensor electrodes. Conventional E. coli DNA detections are facing difficulties in long-preparation-and-detection-time (more than 3 days). Hence, electronic biosensor was introduced by measuring the current-voltage (I–V) DNA probe without amplification of DNA fragments. The detection scheme is based on the interaction between the electron flow on the sensor and the introduction of negative charges from DNA probe and target DNA. The biosensor has a sensitivity of 1.67 × 1013 Ω/M and a wide analytical range. The limit detection is down to 1 × 10−11 M of DNA. The sensor possesses outstanding repeatability and reproducibility and is cabable to detect DNA within 15 min in a minute-volume sample (1 μL).

Fig. (a) Graphical illustration of electronic biosensor set up and (b) relationship between limit of detection (LOD) and the unaffected poultry samples on E. coli O157:H7

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Funding

This work is financially supported by HICOE (phase II) fund–“MEMS for Biomedical Devices (Artificial Kidney)” from Ministry of Education, Modal Insan Fund (MI-2019-005) and Dana Impak Perdana (DIP-2018-006) from Universiti Kebangsaan Malaysia (UKM) and Geran COEMTUN under Grant Nos. 9016-00004, Institute of Nano Electronic Engineering (INEE), Universiti Malaysia Perlis (UniMAP); One-Point Healthlab Sdn. Bhd. and LRGS grant (600-RMI/LRGS 5/3 (3/2013)).

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Authors and Affiliations

  1. Institute of Microengineering and Nanoelectronics, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

    Sh. Nadzirah, Azrul Azlan Hamzah & Chang Fu Dee

  2. Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000, Kangar, Perlis, Malaysia

    U. Hashim, Subash C. B. Gopinath & N. A. Parmin

  3. School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600, Arau, Perlis, Malaysia

    Subash C. B. Gopinath & N. A. Parmin

  4. Department of Materials Science and Engineering, National Chiao Tung University, Hsinchu, 30010, Taiwan

    Hung Wei Yu

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  1. Sh. Nadzirah
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Correspondence to Sh. Nadzirah or Chang Fu Dee.

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Nadzirah, S., Hashim, U., Gopinath, S.C.B. et al. Titanium dioxide–mediated resistive nanobiosensor for E. coli O157:H7. Microchim Acta 187, 235 (2020). https://doi.org/10.1007/s00604-020-4214-y

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