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Electrochemical aptasensor using boron-carbon nanorods decorated by nickel nanoparticles for detection of E. coli O157:H7

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Abstract

The development of a label-free impedimetric aptasensor is reported for rapid and sensitive detection of Escherichia coli O157:H7 employing boron-carbon nanorods decorated by nickel nanoparticles (BC-Ni) nanostructured platform. These highly electroactive BC-Ni nanorods were synthesized to increase the sensitivity of the sensor surface and subsequently functionalized with a specific anti-E. coli O157:H7 aptamer (Kd = 69 nM) as bio-recognition moiety. This fully characterized high-affinity DNA aptamer against the bacteria was selected using a facile microtiter plate-based cell-SELEX methodology. The fabricated electrochemical aptasensor is demonstrated to detect E. coli O157:H7 selectively with a detection limit of 10 cfu and a dynamic detection range of 100 to 105 cfu in water, juice, and fecal samples.

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Acknowledgments

The authors acknowledge Dr. Sharmistha Sinha from INST, Mohali, for providing laboratory facilities and resources for experimental studies.

Funding

The authors acknowledge DST Nanomission Project (No. SR/NM/NS-1510/2014-G) for financial funding. HK and MS acknowledge INST-PhD and CSIR-JRF for fellowships.

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Author notes

  1. Priyanka Sabherwal

    Present address: National Centre for Nanoscience and Nanotechnology, University of Mumbai, 400098, Mumbai, India

Authors and Affiliations

  1. Institute of Nano Science and Technology, 160062, Mohali, India

    Harmanjit Kaur, Munish Shorie & Priyanka Sabherwal

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  1. Harmanjit Kaur
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  2. Munish Shorie
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Correspondence to Priyanka Sabherwal.

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Kaur, H., Shorie, M. & Sabherwal, P. Electrochemical aptasensor using boron-carbon nanorods decorated by nickel nanoparticles for detection of E. coli O157:H7. Microchim Acta 187, 461 (2020). https://doi.org/10.1007/s00604-020-04444-y

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