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Fluorescent assay based on phenyl-modified g-C3N4 nanosheets for determination of thiram

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Abstract

Phenyl-modified graphitic carbon nitride nanosheets (Ph-g-C3N4 NSs) were synthesized by a thermal copolymerization and ultrasonic exfoliation method. The Ph-g-C3N4 NSs are used as a fluorescent assay for determination of thiram. The results of X-ray photoelectron spectroscopy, 13C solid-state nuclear magnetic resonance and Fourier transform infrared spectra confirm that phenyl group is integrated into the heptazine network of g-C3N4. Compared to the g-C3N4 NSs, the Ph-g-C3N4 NSs show bigger stokes shift about 185 nm and higher fluorescence intensity. The fluorescence of Ph-g-C3N4 NSs is quenched by Cu2+ via the photo-induced electron transfer mechanism, which then recovers in the presence of thiram. The fluorescence restoring of Ph-g-C3N4 NSs is correlated with the concentration of thiram. Under the optimized conditions, the fluorescent intensity of g-C3N4 NSs at excitation/emission wavelengths of 310/455 nm give a linear range of 33.0–670 nM with detection limit of 9.90 nM. While fluorescent assay based on the Ph-g-C3N4 NSs show the linear range of 6.70–1300 nM at excitation/emission wavelengths of 310/495 nm with detection limit of 2.01 nM.

Schematic representation of fluorescent “on-off-on” assay based on phenyl-modified graphitic carbon nitride nanosheets (Ph-g-C3N4 NSs) for determination of thiram.

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Acknowledgements

This work was jointly supported by the National Science Foundation of China (Grants 21876125 and 21707105), Zhejiang Provincial Natural Science Foundation (LY19B070010) and Zhejiang Provincial Public Welfare Technology Application Research Project (Grants LGF19B070009 and LY19B070010).

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Authors and Affiliations

  1. Health Assessment Center, Zhejiang Provincial Key Laboratory of Watershed Science and Health, School of Public Health and Management, Wenzhou Medical University, Wenzhou, 325035, People’s Republic of China

    He Mei, Huawei Shu, Mengyu Lv, Wei Liu & Xuedong Wang

  2. State Key Laboratory of Ophthalmology, Optometry and Vision Science, School of Ophthalmology and Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, 325027, People’s Republic of China

    Huawei Shu

  3. Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, 325001, People’s Republic of China

    Huawei Shu

  4. National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou, 215009, People’s Republic of China

    Xuedong Wang

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  1. He Mei
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  2. Huawei Shu
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  4. Wei Liu
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Correspondence to Wei Liu or Xuedong Wang.

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Mei, H., Shu, H., Lv, M. et al. Fluorescent assay based on phenyl-modified g-C3N4 nanosheets for determination of thiram. Microchim Acta 187, 159 (2020). https://doi.org/10.1007/s00604-020-4135-9

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