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A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres

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Abstract

A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The AuNP and PS particles were linked using monoclonal antibodies against influenza virus as the bridge. Under the optimal conditions, the visual detection limit of the AuNP-PS-based strip test was as low as 1/16 hemagglutination unit (HAU). It was 64 times higher than that of 10 nm (4 HAU) AuNP-based strip tests. Quantitative analysis showed that the detection limit of the AuNP-PS-based strip is 0.016 HAU. The AuNP-PS-based strip test showed no cross-reactivity to the other subtypes (H1, H5, H7, or H9) of influenza viruses.

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Funding

This work was supported by grants from the National Key Research and Development Program of China (2016YFD0500701), the Earmarked Fund for Modern Agro-Industry Technology Research System of China (CARS-35), and the Special Fund for Henan Agriculture Research System (S2012-06-02).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China

    Xiao Liu, Ge Li & Gaiping Zhang

  2. Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China

    Xiao Liu, Jifei Yang, Qingmei Li, Yanhong Wang, Jianzhou Shi, Junqing Guo, Ruiguang Deng & Gaiping Zhang

  3. Department of Medicine, Henan Medical College, Zhengzhou, 451191, China

    Xiao Liu

  4. School of Public Health, Xinxiang Medical University, Xinxiang, 453003, China

    Yinbiao Wang

  5. School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China

    Peiyang Ding

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  1. Xiao Liu
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  11. Gaiping Zhang
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Correspondence to Gaiping Zhang.

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Liu, X., Yang, J., Li, Q. et al. A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres. Microchim Acta 187, 306 (2020). https://doi.org/10.1007/s00604-020-04255-1

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