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Simultaneous colorimetric determination of acute myocardial infarction biomarkers by integrating self-assembled 3D gold nanovesicles into a multiple immunosorbent assay

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Abstract

An improved enzyme-free immunosorbent assay is described for the simultaneous detection of the myocardial infarction biomarkers N-terminal pro B type natriuretic peptide (NT-proBNP), creatine kinase-MB (CK-MB), and cardiac muscle troponin T (cTnT). The assay integrates 3D gold nanovesicles (GNVs) and three allochroic agents (phenolphthalein, methyl red, bromothymol blue). The pH regulated allochroic agents were enwrapped in GNVs to acts as ultrasensitive nanoprobes. Loading can be controlled by adjusting the temperature to efficiently load and release the allochroic agents. This bare-eye multicolor assay has limits of detection of 70 pg·mL−1 for NT-proBNP, 910 pg·mL−1 for CK-MB, and 7.8 pg·mL−1 for cTnT. Other features include (a) a linear range that extends over a wide range and sometimes is better than conventional HRP-based immunoassays, and (b) a precision that is comparable to immunofluorescence assays as used in the clinical laboratory.

Schematic presentation of an improved enzyme-free immunosorbent assay (EFISA). It integrates 3D gold nano-vesicles (GNVs) and allochroic agents for the simultaneous detection of acute myocardial infarction (AMI) biomarkers (N-terminal prohormone of brain natriuretic peptide (NT-proBNP), kinase-muscle/brain test (CK-MB), and cardiac muscle troponin (cTnT)).

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Acknowledgements

This research work was financially supported by the National Natural Science Foundation of China (No. 81672112, 81702101), Chongqing Technology Innovation and Application Demonstration Project (cstc2018jscx-msybX0010) and Key Project of Education Department of Sichuan(No. 16ZA0181).

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Authors and Affiliations

  1. Key Laboratory of Laboratory Medical Diagnostics of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Qinli Pu, Tao Dai, Tingyan Yang, Xinying Ou & Guoming Xie

  2. Department of Gastrointestinal Surgery, Second Clinical Medical Institution of North Sichuan Medical College, Nanchong Central Hospital, Nanchong, Sichuan, 637000, People’s Republic of China

    Xuanhua Yang

  3. Clinical Laboratory of Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University, Luzhou, Sichuan, 646000, People’s Republic of China

    Yongcan Guo

  4. University-Town Hospital of Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Junlong Li

  5. Clinical Laboratory of Hospital Affiliated to Chengdu University, Sichuan, 610081, People’s Republic of China

    Shangchun Sheng

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  1. Qinli Pu
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Pu, Q., Yang, X., Guo, Y. et al. Simultaneous colorimetric determination of acute myocardial infarction biomarkers by integrating self-assembled 3D gold nanovesicles into a multiple immunosorbent assay. Microchim Acta 186, 138 (2019). https://doi.org/10.1007/s00604-019-3242-y

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