Abstract
The authors describe an electrochemical immunoassay for ultrasensitive determination of the Mycobacterium tuberculosis (MTb) secretory protein MPT64 which is an antigen for early diagnosis of infection with MTb. Protein G was used to immobilize antibodies against MPT64 on a gold electrode. Graphene oxide with its large surface area was used as a carrier to anchor magnetite (Fe3O4) and platinum (Pt) nanoparticles. The nanocomposite of type GO@Fe3O4@Pt was used as a signal reporter with excellent catalytic activity and recyclability. The nanocomposite exhibits peroxidase-like activity for hydrogen peroxide, best at −0.25 V (vs. Ag/AgCl). It works over a wide range of pH values (2–10) and temperatures (25–65 °C). Further signal amplification strategy was accomplished by rolling circle amplification. This voltammetric assay has a linear response to the logarithm of MPT64 concentration in the range from 5.0 fg·mL−1 to 1.0 ng·mL−1 and a detection limit of 0.34 fg·mL−1 (at a signal to noise ratio of 3, for n = 10). The assay can be completed within 4 h. It was successfully applied to the determination of MPT64 in spiked serum samples. Conceivably, the assay has a large potential in providing laboratory evidence for rapid diagnosis of MTb infection.
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This research work was financially supported by the National Natural Science Foundation of China (No. 81672112).
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Gou, D., Xie, G., Li, Y. et al. Voltammetric immunoassay for Mycobacterium tuberculosis secretory protein MPT64 based on a synergistic amplification strategy using rolling circle amplification and a gold electrode modified with graphene oxide, Fe3O4 and Pt nanoparticles. Microchim Acta 185, 436 (2018). https://doi.org/10.1007/s00604-018-2972-6
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DOI: https://doi.org/10.1007/s00604-018-2972-6