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Colorimetric glutathione assay based on the peroxidase-like activity of a nanocomposite consisting of platinum nanoparticles and graphene oxide

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Abstract

This work describes a colorimetric glutathione (GSH) assay that is taking advantage of (a) the peroxidase-like activity of a nanocomposite prepared from platinum nanoparticles and graphene oxide (PtNP/GO), (b) the decolorizing reaction of GSH with oxidized 3,3′,5,5′-tetramethylbenzidine (TMB), and (c) the GSH-induced scavenging of hydroxy radicals produced from H2O2. The assay has a detection limit as low as 4 nM of GSH. It is flexible because it involves a single step reaction. The method was successfully applied to the determination of GSH in hemolyzed human blood. The concentrations determined by this method in GSH-spiked blood samples ranged from 95.0 to 112.5%, with relative standard deviations between 0.5 and 4.2%.

Schematic of a rapid and sensitive colorimetric method is developed for the detection of GSH in hemolyzed human blood. It is based on the peroxidase-mimicking property of a platinum nanoparticle/graphene oxide (PtNP/GO) composite and the inhibitory effect of GSH.

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Acknowledgements

We sincerely acknowledge the financial support of the National Natural Science Foundation of China (21175023, 21675024), the Program for Innovative leading talents in Fujian Province (2016B016), and Joint Funds for the Innovation of Science and Technology, Fujian Province (2016Y9056).

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Author notes

  1. Hui-Huang Xu and Hao-Hua Deng contributed equally to this work.

Authors and Affiliations

  1. Department of Pediatric Surgery, Fujian Provincial Hospital, Fuzhou, 350001, China

    Hui-Huang Xu

  2. Nano Medical Technology Research Institute, Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou, 350004, China

    Hao-Hua Deng, Xiao-Qing Lin, Yan-Yu Wu, Xiu-Ling Lin, Hua-Ping Peng, Ai-Lin Liu & Wei Chen

  3. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China

    Xing-Hua Xia

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  1. Hui-Huang Xu
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Correspondence to Wei Chen.

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Xu, HH., Deng, HH., Lin, XQ. et al. Colorimetric glutathione assay based on the peroxidase-like activity of a nanocomposite consisting of platinum nanoparticles and graphene oxide. Microchim Acta 184, 3945–3951 (2017). https://doi.org/10.1007/s00604-017-2429-3

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  • DOI: https://doi.org/10.1007/s00604-017-2429-3

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