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A gold electrode modified with a three-dimensional graphene-DNA composite for sensitive voltammetric determination of dopamine

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Abstract

The authors describe a three-dimensional (3D) structure composed of graphene and DNA for use in a voltammetric dopamine (DA) sensor. The material was deposited on a gold electrode, and the enhanced charge-transfer performance and deposition of DNA were confirmed by electrochemical analysis. Atomic force microscopy shows the graphene-DNA composite to have been assembled on the modified gold electrode. The modified gold electrode possesses excellent electrocatalytic activity for determination of DA, best at a working voltage of 0.1 V (vs. SCE). Ascorbic acid does not interfere. Response to DA is linear in the 0.1 to 100 μM concentration range, with a 30 nM detection limit even in the presence of 1.0 mM of ascorbic acid.

Schematic of a voltammetric dopamine sensor based on the use of a 3D structure composed of graphene and DNA. The sensor displays an enhanced charge-transfer rate, excellent electrocatalytic activity towards dopamine, and a 30 nM detection limit.

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References

  1. Xu T, Zhang Q, Zheng J, Lv Z, Wei J, Wang A, Feng J (2014) Simultaneous determination of dopamine and uric acid in the presence of ascorbic acid using Pt nanoparticles supported on reduced graphene oxide. Electrochim Acta 115:109–115

    Article  CAS  Google Scholar 

  2. Sanghavi B, Wolfbeis O, Hirsch T, Swami N (2015) Nanomaterial-based electrochemical sensing of neurological drugs and neurotransmitters. Microchim Acta 182:1–41

    Article  CAS  Google Scholar 

  3. Geim, A.K., Novoselov, K.S (2007) The rise of graphene. Nat Mater 6(3):183–191

  4. Khomyakov PA, Giovannetti G, Rusu PC, Brocks GV, Van den Brink J, Kelly PJ (2009)) First-principles study of the interaction and charge transfer between graphene and metals. Phys Rev B 79(19):195425

    Article  Google Scholar 

  5. Slinker, J. D., Muren, N.B., Renfrew E. S.; Barton, J. K (2011) DNA charge transport over 34 nm. Nat Chem 3:228–233

  6. Premkumar, T., Geckeler, K.E (2012) Graphene–DNA hybrid materials: assembly, applications, and prospects. Prog Polym Sci 37(4):515–529

  7. Lee, S.H., Kim, H.W., Hwang, J.O., Lee, W.J., Kwon, J., Bielawski, C.W., Ruoff, R.S., Kim, S.O (2010) Three-dimensional self-assembly of graphene oxide platelets into mechanically flexible Macroporous carbon films. Angew Chem 122(52): 10282–10286

  8. Xu Y, Wu Q, Sun Y, Bai H, Shi G (2010) Three-dimensional self-assembly of graphene oxide and DNA into multifunctional hydrogels. ACS Nano 4(12):7358–7362

    Article  CAS  Google Scholar 

  9. Sun X, Liu Z, Welsher K, Robinson JT, Goodwin A, Zaric S, Dai H (2008) Nano-graphene oxide for cellular imaging and drug delivery. Nano Res 1(3):203–212

    Article  CAS  Google Scholar 

  10. Liu X, Aizen R, Freeman R, Yehezkeli O, Willner I (2012) Multiplexed aptasensors and amplified DNA sensors using functionalized graphene oxide: application for logic gate operations. ACS Nano 6(4):3553–3563

    Article  CAS  Google Scholar 

  11. He Q, Wu S, Yin Z, Zhang H (2012) Graphene-based electronic sensors. Chem Sci 3(6):1764–1772

    Article  CAS  Google Scholar 

  12. Patil AJ, Vickery JL, Scott TB, Mann S (2009) Aqueous stabilization and self-assembly of graphene sheets into layered bio-nanocomposites using DNA. Adv Mater 21(31):3159–3164

    Article  CAS  Google Scholar 

  13. Subramanian P, Lesniewski A, Kaminska I, Vlandas A, Vasilescu A, Niedziolka-Jonsson J, Pichonat E, Happy H, Boukherroub R, Szunerits S (2013) Lysozyme detection on aptamer functionalized graphene-coated SPR interfaces. Biosens Bioelectron 50:239–243

    Article  CAS  Google Scholar 

  14. Sarma SD, Adam S, Hwang EH, Rossi E (2011) Electronic transport in two-dimensional graphene. Rev Mod Phys 83(2):407

    Article  Google Scholar 

  15. Chen D, Tang L, Li J (2010) Graphene-based materials in electrochemistry. Chem Soc Rev 39(8):3157–3180

    Article  CAS  Google Scholar 

  16. Kim Y, Bong S, Kang Y, Yang Y, Mahajan RK, Kim JS, Kim H (2010) Electrochemical detection of dopamine in the presence of ascorbic acid using graphene modified electrodes. Biosens Bioelectron 25(10):2366–2369

    Article  CAS  Google Scholar 

  17. Sheng Z, Zheng X, Xu J, Bao W, Wang F, Xia X (2012) Electrochemical sensor based on nitrogen doped graphene: simultaneous determination of ascorbic acid, dopamine and uric acid. Biosens Bioelectron 34(1):125–131

    Article  CAS  Google Scholar 

  18. Wang Y, Li Y, Tang L, Lu J, Li J (2009) Application of graphene-modified electrode for selective detection of dopamine. Electrochem Commun 11(4):889–892

    Article  CAS  Google Scholar 

  19. Wu L, Feng L, Ren J, Qu X (2012) Electrochemical detection of dopamine using porphyrin-functionalized graphene. Biosens Bioelectron 34(1):57–62

    Article  Google Scholar 

  20. Caballero-Diaz, E., Benitez-Martinez, S., Valcarcel, M., Rapid and simple nanosensor by combination of graphene quantum dots and enzymatic inhibition mechanisms, Sens Actuators B Chem, 2017, 240, 90–99

  21. Lin XQ, Lu LP, Jiang XH (2003) Voltammetric behavior of dopamine at ct-DNA modified carbon fiber microelectrode. Microchim Acta 143:229–235

    Article  CAS  Google Scholar 

  22. Lu LP, Wang SQ, Lin XQ (2004) Fabrication of layer-by-layer deposited multilayer films containing DNA and gold nanoparticle for norepinephrine biosensor. Anal Chim Acta 519:161–166

    Article  CAS  Google Scholar 

  23. Furst, A.L., Hill, M.G., Barton, J.K (2013) DNA-modified electrodes fabricated using copper-free click chemistry for enhanced protein detection. Langmuir 29(52): 16141–16149

  24. Lu LP, Xu LH, Kang TF, Cheng SY (2012) Investigation of DNA damage treated with perfluorooctane sulfonate (PFOS) on ZrO2/DDAB active nano-order film. Biosens Biolectron 35:180–185

    Article  CAS  Google Scholar 

  25. Xie R, Wang Z, Zhou W, Liu Y, Fan L, Li Y, Li X (2016) Graphene quantum dots as smart probes for biosensing. Anal Methods 8:4001–4016

    Article  CAS  Google Scholar 

  26. Laviron E (1979) General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems. J Electroanal Chem Interfacial Electrochem 101(1):19–28

    Article  CAS  Google Scholar 

  27. Song Y, Wan L, Wang Y, Zhao S, Hou H, Wang L (2012) Electron transfer and electrocatalytics of cytochrome c and horseradish peroxidase on DNA modified electrode. Bioelectrochemistry 85:29–35

    Article  CAS  Google Scholar 

  28. sPheeney, C.G., Barton, J.K (2013) Intraduplex DNA-mediated electrochemistry of covalently tethered redox-active reporters. J Am Chem Soc 135(40): 14944–14947

  29. Pattnaik S, Swain K, Lin ZQ (2016) Graphene and graphene-based nanocomposites: biomedical applications and biosafety. J Mater Chem B 4:7813–7831

    Article  CAS  Google Scholar 

  30. Liu S, Yan J, He G, Zhong D, Chen J, Shi L, Zhou X, Jiang H (2012) Layer-by-layer assembled multilayer films of reduced graphene oxide/gold nanoparticles for the electrochemical detection of dopamine. J Electroanal Chem 672:40–44

    Article  CAS  Google Scholar 

  31. Wang H, Ren F, Yue R, Wang C, Zhai C, Du Y (2014) Macroporous flower-like graphene-nanosheet clusters used for electrochemical determination of dopamine. Colloids Surf A Physicochem Eng Asp 448:181–185

    Article  CAS  Google Scholar 

  32. Feng X, Zhang Y, Zhou J, Li Y, Chen S, Zhang L, Ma Y, Wang L, Yan X (2015) Three-dimensional nitrogen-doped graphene as an ultrasensitive electrochemical sensor for the detection of dopamine. Nanoscale 7(6):2427–2432

    Article  CAS  Google Scholar 

  33. Han HS, Ahmed MS, Jeong H, Jeon S (2015) The determination of dopamine in presence of serotonin on dopamine-functionalized electrochemically prepared graphene biosensor. J Electrochem Soc 162(4):B75–B82

    Article  CAS  Google Scholar 

  34. Sun J, Li L, Zhang X, Liu D, Lv S, Zhu D, Wu T, You T (2015) Simultaneous determination of ascorbic acid, dopamine and uric acid at a nitrogen-doped carbon nanofiber modified electrode. RSC Adv 5(16):11925–11932

    Article  CAS  Google Scholar 

  35. Wang Y, Zhang Y, Hou C, Liu M (2016) Ultrasensitive electrochemical sensing of dopamine using reduced graphene oxide sheets decorated with p-toluenesulfonate-doped polypyrrole/Fe3O4 nanospheres. Microchim Acta 183(3):1145–1152

    Article  CAS  Google Scholar 

  36. Mani V, Govindasamy M, Chen SM, Karthik R, Huang ST (2016) Determination of dopamine using a glassy carbon electrode modified with a graphene and carbon nanotube hybrid decorated with molybdenum disulfide flowers. Microchim Acta 183:2267–2275

    Article  CAS  Google Scholar 

  37. Rao D, Zhang X, Sheng Q, Zheng J (2016) Highly improved sensing of dopamine by using glassy carbon electrode modified with MnO2, graphene oxide, carbon nanotubes and gold nanoparticles. Microchim Acta 183(9):2597–2604

    Article  CAS  Google Scholar 

  38. Liu R, Zeng X, Liu J, Luo J, Zheng Y, Liu X (2016) A glassy carbon electrode modified with an amphiphilic, electroactive and photosensitive polymer and with multi-walled carbon nanotubes for simultaneous determination of dopamine and paracetamol. Microchim Acta 183:1543–1551

    Article  CAS  Google Scholar 

  39. Zhang ZL, Feng JX, Wang AJ, Wei J, Lv ZY, Feng JJ (2015) A glassy carbon electrode modified with porous gold nanosheets for simultaneous determination of dopamine and acetaminophen. Microchim Acta 182:589–595

    Article  CAS  Google Scholar 

  40. Xu Y, Hun X, Liu F, Wen X, Luo X (2015) Aptamer biosensor for dopamine based on a gold electrode modified with carbon nanoparticles and thionine labeled gold nanoparticles as probe. Microchim Acta 182:1797–1802

    Article  CAS  Google Scholar 

  41. Hammami A, Sahli R, Raouafi N (2016) Indirect amperometric sensing of dopamine using a redox-switchable naphthoquinone-terminated self-assembled monolayer on gold electrode. Microchim Acta 183:1137–1144

    Article  CAS  Google Scholar 

  42. Kumar MK, Prataap RV, Mohan S, Jha SK (2016) Preparation of electro-reduced graphene oxide supported walnut shape nickel nanostructures, and their application to selective detection of dopamine. Microchim Acta 183(5):1759–1768

    Article  CAS  Google Scholar 

  43. Qian T, Wu SS, Shen J (2013) Facilely prepared polypyrrole-reduced graphite oxide core-shell microspheres with high dispersibility for electrochemical detection of dopamine. Chem Commun 49:4610–4612

    Article  CAS  Google Scholar 

  44. Zhu WC, Chen T, Ma XM, Ma HY, Chen SH (2013) Highly sensitive and selective detection of dopamine based on hollow gold nanoparticles-graphene nanocomposite modified electrode. Colloids Surf B: Biointerfaces 111:321–326

    Article  CAS  Google Scholar 

  45. Yang L, Liu D, Huang J et al (2014) Simultaneous determination of dopamine, ascorbic acid and uric acid at electrochemically reduced graphene oxide modified electrode. Sensors Actuators B Chem 193:166–172

    Article  CAS  Google Scholar 

  46. Palanisamy S, Ku S, Chen SM (2013) Dopamine sensor based on a glassy carbon electrode modified with a reduced graphene oxide and palladium nanoparticles composite. Microchim Acta 180:1037–1042

    Article  CAS  Google Scholar 

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 21475006, 21375005, 21527808).

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Authors and Affiliations

  1. Key Laboratory of Beijing on Regional Air Pollution Control, Beijing University of Technology, Beijing, 100124, China

    Liping Lu, Linqing Guo, Tianfang Kang & Shuiyuan Cheng

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  1. Liping Lu
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Correspondence to Liping Lu.

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Lu, L., Guo, L., Kang, T. et al. A gold electrode modified with a three-dimensional graphene-DNA composite for sensitive voltammetric determination of dopamine. Microchim Acta 184, 2949–2957 (2017). https://doi.org/10.1007/s00604-017-2267-3

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  • DOI: https://doi.org/10.1007/s00604-017-2267-3

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