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Identification of genetically modified DNA found in Roundup Ready soybean using gold nanoparticles

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Abstract

The authors describe an SPR sensor chip coated with gold nanoparticles (AuNPs) that enables highly sensitive determination of genetically modified (GM) crops. Detection is based on localized surface plasmon resonance (LSPR) with its known sensitivity to even minute changes in refractive index. The device consists of a halogen light source, a light detector, and a cuvette cell that contains a sensor chip coated with AuNPs. It is operated in the transmission mode of the optical path to enhance the plasmonic signal. The sample solution containing target DNA (e.g. from the GM crop) is introduced into the cuvette with the sensor chip whose surface was functionalized with a capture DNA. Following a 30-min hybridization, the changes of the signal are recorded at 540 nm. The chip responds to target DNA in the 1 to 100 nM concentration range and has a 1 nM detection limit. Features of this sensor chip include a short reaction time, ease of handling, and portability, and this enables on-site detection and in-situ testing.

A localized surface plasmon resonance (LSPR)-based nanoplasmonic spectroscopic device enabling a highly sensitive biosensor is developed for the detection of genetically modified (GM) DNA founded in Roundup Ready (RR) soybean.

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Acknowledgments

This work was supported by the Pioneer Research Center Program (NRF-2012-0009575) and National Research Foundation Grant (NRF-2012R1A1B4002413, NRF-2014R1A1A2059341, NRF-2016R1A2B4006987) from National Research Foundation of Korea. This work was also partially supported by Inha University Research Grant.

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    Authors and Affiliations

    1. Department of Biological Engineering, Inha University, 100 Inharo, Nam-gu, Incheon, 22212, South Korea

      Huisoo Jang, Cheol Hwan Kwak, Yun Suk Huh & Tae-Joon Jeon

    2. Biohybrid Systems Research Center (BSRC), Inha University, 100 Inharo, Nam-gu, Incheon, 22212, South Korea

      Huisoo Jang, Sun Min Kim, Yun Suk Huh & Tae-Joon Jeon

    3. Plexense Inc, 75 Techno 1-ro, Daejeon, 34014, South Korea

      Gibum Kim

    4. Department of Mechanical Engineering, Inha University, 100 Inharo, Nam-gu, Incheon, 22212, South Korea

      Sun Min Kim

    5. Convergent Research Center for Metabolism and Immunoregulation (CRCMI), Inha University, 100 Inharo, Nam-gu, Incheon, 22212, South Korea

      Sun Min Kim & Tae-Joon Jeon

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    1. Huisoo Jang
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    2. Cheol Hwan Kwak
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    Correspondence to Yun Suk Huh or Tae-Joon Jeon.

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    Huisoo Jang and Cheol Hwan Kwak contributed equally to this work

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    Jang, H., Kwak, C.H., Kim, G. et al. Identification of genetically modified DNA found in Roundup Ready soybean using gold nanoparticles. Microchim Acta 183, 2649–2654 (2016). https://doi.org/10.1007/s00604-016-1899-z

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    • DOI: https://doi.org/10.1007/s00604-016-1899-z

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