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Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots

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Abstract

Cadmium-telluride quantum dots (QDs) were conjugated to an antibody (Ab) against Citrus tristeza virus (CTV), while the coat protein (CP) of the CTV was immobilized on the surface of carbon nanoparticles (CNPs). Following immunobinding of the QD-Ab and the CP-loaded CNPs, the fluorescence of the CdTe QDs was quenched by the CNPs. This effect was exploited to design a detection assay for the CTV which was found more sensitive and specific than the existing enzyme linked immunosorbent assay (ELISA). The limit of detection was measured at about 220 ng⋅ mL‾1 of CTV. The Stern-Volmer plot of the CNPs-QD quencher pair showed a positive deviation from linearity which was ascribed to the presence of both static and dynamic quenching.

Cadmium-telluride quantum dots (QDs) were conjugated to an antibody (Ab) against Citrus tristeza virus (CTV), while the coat protein (CP) of the CTV was immobilized on the surface of carbon nanoparticles (CNPs). Following immunobinding of the QD-Ab and the CP-loaded CNPs, the fluorescence of the CdTe QDs was quenched by the CNPs. This effect was exploited to design a detection assay for the CTV which was found more sensitive and specific than the existing enzyme linked immunosorbent assay (ELISA).

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Authors and Affiliations

  1. Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Taha R. Shojaei

  2. Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Mohamad A. Mohd Salleh

  3. Department of Plant Protection, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Kamaruzaman Sijam

  4. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Raha A. Rahim

  5. Research and Development Department, Nanozino, Tehran, 16536-43181, Iran

    Afshin Mohsenifar

  6. Department of Plant Viruses, Iranian Institute of Plant Protection, Tehran, Iran

    Reza Safarnejad

  7. Nanosystems Research Team (NRTeam), Microbial Biotechnology and Biosafety Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran

    Meisam Tabatabaei

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  1. Taha R. Shojaei
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Correspondence to Taha R. Shojaei or Mohamad A. Mohd Salleh.

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Shojaei, T.R., Salleh, M.A.M., Sijam, K. et al. Fluorometric immunoassay for detecting the plant virus Citrus tristeza using carbon nanoparticles acting as quenchers and antibodies labeled with CdTe quantum dots. Microchim Acta 183, 2277–2287 (2016). https://doi.org/10.1007/s00604-016-1867-7

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