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Multiple enhancement of luminol electrochemiluminescence using electrodes functionalized with titania nanotubes and platinum black: ultrasensitive determination of hydrogen peroxide, resveratrol, and dopamine

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Abstract

We describe a substantial improvement of the electrochemiluminescence (ECL) of luminol which is widely used in flow injection analysis (FIA). It is based on synchronous dual sensitization of ECL by using titania nanotubes (TiNTs) and platinum black (PB). A piece of indium tin oxide (ITO) glass functionalized with TiNTs acts as the first working electrode, and a PB-modified platinum plate serves as the second one. By applying two constant potentials to the two electrodes, strong and consecutive ECL emission of luminol is obtained. The system works well in assays as shown for the successful quantitation of hydrogen peroxide (H2O2), of the antioxidant resveratrol, and of the neutrotransmitter dopamine (DA) in spiked human serum samples. The detection limits for these three species (at a signal-to-noise ratio of 3) are as low as 66 pM (H2O2), 22 nM (resveratrol), and 30 nM (DA). Recoveries in assays of DA in spiked serum range from 97.3 to 105.4 %. In our perception, the technique of dual sensitization represents a substantial improvement of the detection limits of ECL assays.

A substantial improvement of the electrochemiluminescence (ECL) of luminol was accomplished by synchronous dual sensitization of ECL with using titania nanotubes and platinum black.

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (21175096, 21375091); The Project of Scientific and Technologic Infrastructure of Suzhou (SZS201207).

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Authors and Affiliations

  1. Institute of Analytical Chemistry; The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, Soochow University, Dushu Lake Higher Education Town, Industrial Park, 215123, Suzhou, People’s Republic of China

    Liang Ming, Tingting Peng & Yifeng Tu

  2. College of Chemistry and Chemical Engineering, Nantong University, Nantong, 226019, People’s Republic of China

    Liang Ming

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  1. Liang Ming
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  2. Tingting Peng
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  3. Yifeng Tu
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Correspondence to Liang Ming or Yifeng Tu.

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Ming, L., Peng, T. & Tu, Y. Multiple enhancement of luminol electrochemiluminescence using electrodes functionalized with titania nanotubes and platinum black: ultrasensitive determination of hydrogen peroxide, resveratrol, and dopamine. Microchim Acta 183, 305–310 (2016). https://doi.org/10.1007/s00604-015-1614-5

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  • DOI: https://doi.org/10.1007/s00604-015-1614-5

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