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Enhanced luminol electrochemiluminescence triggered by an electrode functionalized with dendrimers modified with titanate nanotubes

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Abstract

We have constructed a novel electrochemiluminescence (ECL) platform by functionalizing a poly(amidoamine) dendrimer (PAAD) with titanate nanotubes (TiNTs). The PAAD has an open spherical structure that possesses a high density of active groups and thus favors mass transport, while the TiNTs possess excellent electronic conductivity and thus can promote electron transfer on the surface of a glassy carbon electrode (GCE). A study on the intensity and stability of the ECL of luminol on the modified GCE revealed a substantial improvement compared to that of a bare GCE. The effects of the concentration of TiNTs, the pH value of the solution, and of electrochemical parameters on the intensity of the ECL of luminol were studied and resulted in a sensitive ECL sensor for hydrogen peroxide (H2O2) that works in the concentration range of 1 nM to 0.9 μM. The scavenging effect of superoxide dismutase (SOD) on the H2O2 electrode ECL was then exploited to design a biosensor for the determination of SOD in concentrations between 50 and 500 nM.

The reaction mechanism schematic diagram of luminol ECL on the PAAD/TiNTs modified eledtrode.

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Acknowledgment

This project was financially supported by the National Nature Sciences Foundation of China (21205016, 21274022), the Program for Changjiang Scholars and Innovative Research Teams in University (No. IRT1116), the National Science Foundation of Fujian Province (2011 J05020), and the Education Department of Fujian Province (JA11062). Fujian Normal University Outstanding Young Teacher Research Fund Projects (fjsdjk2012068) were also greatly acknowledged.

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Authors and Affiliations

  1. Key Laboratory of Humid Subtropical Ecogeographical Process of the Ministry of Education, College of Geographical Science, Fujian Normal University, Fuzhou, 350007, China

    Yanyu Lin & Yusheng Yang

  2. College of Chemistry and Chemical Engineering, Fujian Normal University, Fuzhou, Fujian, 350007, China

    Yanyu Lin, Hong Dai, Guifang Xu, Ting Yang & Yuejin Tong

  3. Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China

    Caiping Yang & Guonan Chen

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  1. Yanyu Lin
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  2. Hong Dai
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  3. Guifang Xu
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  4. Ting Yang
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  5. Caiping Yang
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  6. Yuejin Tong
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  7. Yusheng Yang
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  8. Guonan Chen
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Correspondence to Hong Dai or Guonan Chen.

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Lin, Y., Dai, H., Xu, G. et al. Enhanced luminol electrochemiluminescence triggered by an electrode functionalized with dendrimers modified with titanate nanotubes. Microchim Acta 180, 563–572 (2013). https://doi.org/10.1007/s00604-013-0963-1

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