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Ultrasensitive and selective voltammetric aptasensor for dopamine based on a conducting polymer nanocomposite doped with graphene oxide

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Abstract

We describe an aptasensor for the determination of dopamine in human serum and with ultrahigh sensitivity and selectivity. The sensor is based on a nanocomposite consisting of reduced graphene oxide (rGO) and the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT). The PEDOT/rGO interface was prepared by electrochemical polymerization of EDOT using graphene oxide as the dopant which is later electrochemically reduced to form rGO. Subsequent covalent modification of the high surface area composite with a selective aptamer enables highly sensitive and selective detection by differential pulse voltammetry. The calibration plot established at a working voltage of 160 mV displays a linear response in the 1 pM to 160 nM concentration range and an unprecedented detection limit of 78 fM. The sensor is fairly selective in not responding to common interferents, and is reusable after regeneration with a 7 M solution of urea. It was successfully applied to (spiked) serum samples and gave recoveries ranging from 98.3 to 100.7 %.

A highly sensitive and selective biosensor for dopamine was developed based on an aptamer-modifed conducting polymer (PEDOT) doped with reduced graphene oxide.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 21275087, 21175077), the Natural Science Foundation of Shandong Province of China (ZR2012BM008), and the Taishan Scholar Program of Shandong Province, China.

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Authors and Affiliations

  1. Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China

    Wenting Wang, Wei Wang & Xiliang Luo

  2. Department of Chemistry, University of Oxford, Oxford, OX1 3QZ, UK

    Jason J. Davis

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  1. Wenting Wang
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  2. Wei Wang
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  3. Jason J. Davis
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  4. Xiliang Luo
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Correspondence to Jason J. Davis or Xiliang Luo.

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Wang, W., Wang, W., Davis, J.J. et al. Ultrasensitive and selective voltammetric aptasensor for dopamine based on a conducting polymer nanocomposite doped with graphene oxide. Microchim Acta 182, 1123–1129 (2015). https://doi.org/10.1007/s00604-014-1418-z

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  • DOI: https://doi.org/10.1007/s00604-014-1418-z

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