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Fragment-imprinted microspheres for the extraction of sulfonamides

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Abstract

Fragment-imprinted microspheres (FIMs) were synthesized by suspension polymerization of 4-sulfa-6-chloropyrimidine (the template), methacrylic acid and styrene (the mixed functional monomers), divinylbenzene (the crosslinker), and azobisisobutyronitrile (the initiator). The optimum conditions were obtained by an orthogonal experiment. After removal of the templates, the microspheres serve as a material to fairly specifically bind sulfonamides, the absorption capacity being 6.32 mg g–1, whereas the absorption by non-imprinted microspheres is 1.68 mg g−1. A method was worked out for the simultaneous determination of five sulfonamides by solid phase extraction (using the FIMs) coupled to HPLC, and applied to analyze milk samples. The recoveries are within 87.6 and 96.5 %, with relative standard deviations between 1.25 and 2.89 %.

Graphical abstract showed that fragment imprinted-solid phase extraction had the best selective adsorption performance of the five sulfonamide antibiotics compare with C18, Silica and Florisil column. It was satisfactory by using fragment imprinted microspheres as sorbents of solid phase extraction for enriching and separating target compounds from complex matrices.

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Acknowledgments

The authors gratefully appreciate the financial support from the following research funds: Key Scientific and Technological Project of Henan Province (112102310030), Natural Science Basic Research Plan in the Education Department of Henan Province (2010A150006), Plan of Nature Science Fundamental Research in Henan University of Technology and Education Innovation Foundation for the Postgraduate of Henan University of Technology (11YJCX08).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, People’s Republic of China

    Juan He, Hongzhe Tang, Liqin You, Haijun Zhan, Jing Zhu & Kui Lu

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  1. Juan He
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  2. Hongzhe Tang
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  3. Liqin You
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  4. Haijun Zhan
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Correspondence to Juan He.

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He, J., Tang, H., You, L. et al. Fragment-imprinted microspheres for the extraction of sulfonamides. Microchim Acta 180, 903–910 (2013). https://doi.org/10.1007/s00604-013-1011-x

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  • DOI: https://doi.org/10.1007/s00604-013-1011-x

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