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Determination of sulfamethoxazole in milk using molecularly imprinted polymer monolith microextraction coupled to HPLC

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Abstract

We report on a new method for the selective extraction of the antibiotic sulfamethoxazole (SMO) in milk that is making use of a molecularly imprinted polymer (MIP) monolith as the sorbent. The monolith was synthesized in the tip of a micropipette using SMO as the template and a combination of acrylamide and 4-vinylpyridine as the co-functional monomers. The monolith was connected to syringes in different sizes and used for microextraction without any other treatment and showed high selectivity and enrichment ability for SMO. It was applied to the selective extraction and sensitive determination of SMO in milk. The linear range is from 5–600 μg L−1, the correlation coefficient (r2) is 0.9984, and the detection limit (at S/N = 3) is 1 μg L−1. Recoveries range from 93.6 to 101.7 %, with relative standard deviations of <6.1 %.

A method for the selective extraction of sulfamethoxazole (SMO) in milk based on molecularly imprinted polymer (MIP) monolith as the sorbent was developed. The linear ranges were 5–600 μg/L for SMO in milk. High recoveries of 93.6 ∼ 101.7 % from milk were obtained with relative standard deviations less than 6.1 %.

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Acknowledgments

This work was supported by the Natural Science Fund for Creative Research Groups of Hubei Province of China (grant no. 2011CDA111), and the Education Commission of Hubei Province of China (grant no. T201101, D20120106), the National Nature Science Foundation of China (grant no. 20975030, 20835004, 21003034). The authors would like to thank their colleagues for their valuable technical assistance.

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Authors and Affiliations

  1. Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People’s Republic of China

    Na Sun, Shengli Wu, Huaixia Chen, Daijun Zheng, Junwen Xu & Yong Ye

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  1. Na Sun
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  2. Shengli Wu
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  3. Huaixia Chen
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Correspondence to Huaixia Chen.

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Sun, N., Wu, S., Chen, H. et al. Determination of sulfamethoxazole in milk using molecularly imprinted polymer monolith microextraction coupled to HPLC. Microchim Acta 179, 33–40 (2012). https://doi.org/10.1007/s00604-012-0862-x

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  • DOI: https://doi.org/10.1007/s00604-012-0862-x

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