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Detection of Helicobacter pylori with a nanobiosensor based on fluorescence resonance energy transfer using CdTe quantum dots

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Abstract

We report on a method for the sensitive determination of Helicobacter that is based on fluorescence resonance energy transfer using two oligonucleotide probes labeled with CdTe quantum dots (QDs) and 5-carboxytetramethylrhodamine (Tamra) respectively. QDs labeled with an amino-modified first oligonucleotide, and a Tamra-labeled second oligonucleotide were added to the DNA targets upon which hybridization occurred. The resulting assembly brings the Tamra fluorophore (the acceptor) and the QDs (the donor) into close proximity and causes fluorescence resonance energy transfer (FRET) to occur upon photoexcitation of the donor. In the absence of target DNA, on the other hand, the probes are not ligated, and no emission by the Tamra fluorophore is produced due to the lack of FRET. The feasibility of the method was demonstrated by the detection of a synthetic 210-mer nucleotide derived from Helicobacter on a nanomolar level. This homogeneous DNA detection scheme is simple, rapid and efficient, does not require excessive washing and separation steps, and is likely to be useful for the construction of a nanobiosensor for Helicobacter species.

We report a method for the sensitive determination of Helicobacter that is based on fluorescence resonance energy transfer using two oligonucleotide probes labeled with CdTe quantum dots and 5-carboxytetramethylrhodamine respectively.

The feasibility of the method was demonstrated by the detection of a synthetic 210-mer nucleotide derived from Helicobacter on a nanomolar level. This homogeneous DNA detection scheme is simple, rapid and efficient, does not require excessive washing and separation steps, and is likely to be useful for the construction of a nanobiosensor for Helicobacter species.

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Authors and Affiliations

  1. Deptartment of Chemistry, Tarbiat Modares University, Tehran, 4641776489, Iran

    Maryam Shanehsaz

  2. Deptartment of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 4641776489, Iran

    Afshin Mohsenifar

  3. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, 4641776489, Tehran, Iran

    Sadegh Hasannia

  4. Department of Biochemistry, Faculty of Biology, Guilan University, Rasht, 4199613769, Iran

    Nazanin Pirooznia

  5. Department of Chemistry, Faculty of Basic Sciences, Kharazmi University, Tehran, 1571914911, Iran

    Yasaman Samaei

  6. Department of Chemistry, Razi University, Kermanshah, 6714967346, Iran

    Mojtaba Shamsipur

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  1. Maryam Shanehsaz
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  2. Afshin Mohsenifar
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  4. Nazanin Pirooznia
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Correspondence to Afshin Mohsenifar or Mojtaba Shamsipur.

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Shanehsaz, M., Mohsenifar, A., Hasannia, S. et al. Detection of Helicobacter pylori with a nanobiosensor based on fluorescence resonance energy transfer using CdTe quantum dots. Microchim Acta 180, 195–202 (2013). https://doi.org/10.1007/s00604-012-0906-2

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  • DOI: https://doi.org/10.1007/s00604-012-0906-2

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