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DNA-Labeled Gold-Based Optical Nanobiosensor Monitoring DNA–Mitoxantrone Interaction

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Abstract

The present study focuses on the construction of optical nanobiosensor to determine toxicological behavior of mitoxantrone (MTX), an anti-tumor drug. For this purpose, gold nanoparticles (AuNPs) were labeled with DNA, respectively combining the physicochemical detector component with the biological analytical component. Fluorescence resonance energy transfer (FRET) phenomenon works as the basic principle for the determination of MTX–DNA interaction by spectrofluorometry. Mitoxantrone intercalates with the DNA and produces MTX-DNA adduct, which restricts protein synthesis and causes excessive production of free radicals in the myocardium that could eventually lead to cardiac arrest. Mapping the adverse reaction of MTX with DNA at molecular level, a conformational change within the nanobiosensor complex was evident that increased the distance between the fluorescent/quencher molecules. The consequent changes in the fluorescence spectrum of the sensor due to FRET modulation by varying concentrations of MTX proved the basis of the tox-screen. Paracetamol, an analgesic agent, was used as controlled drug in this study. Results have demonstrated that the optical nanobiosensor is rapid and sensitive with a detection limit up to 1 μg of MTX interaction, illustrating how it is a feasible technique for surveillance of drug–DNA interaction in molecular toxicology.

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Acknowledgments

The authors would like to acknowledge Cipla Ltd (Mumbai, India) for providing mitoxantrone for monitoring toxicological studies by Nanodevice. We are highly indebted to Bjorn Akerman, Department of Chemistry and Bioscience, Chalmers University of Technology, Kemivagen, Goteborg, Sweden for providing necessary information on DNA–AuNP assembly.

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  1. Institute of Research and Development, Gujarat Forensic Sciences University, Near Police Bhavan, Sector 18A, Gandhinagar, India

    Amitkumar Lad & Y. K. Agrawal

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  1. Amitkumar Lad
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  2. Y. K. Agrawal
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Correspondence to Amitkumar Lad.

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Lad, A., Agrawal, Y.K. DNA-Labeled Gold-Based Optical Nanobiosensor Monitoring DNA–Mitoxantrone Interaction. BioNanoSci. 2, 9–15 (2012). https://doi.org/10.1007/s12668-011-0030-5

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