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Dansyl Chloride as a Derivatizing Agent for the Analysis of Biogenic Amines by CZE-UV

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Abstract

Biogenic amines (BAs) are important compounds that can be used in the quality control of food and beverages. BA analysis is a challenging task that can be made easier by applying a derivatizing agent like dansyl chloride (DNS). The optimized capillary zone electrophoresis (CZE) separation of the DNS-BA derivates (derivates of cadaverine, histamine, putrescine, tryptamine, and tyramine) was performed using benzylamine as an internal standard, a potential of 18 kV, a temperature of 23 °C, a running buffer consisting of phosphoric acid, 120 mmol L−1, pH 2.5, and an hydrodynamic injection at 25 mBar for 6 s. All calibration curves had r2 higher than 0.99, and limits of detection (LODs) ranged from 7 to 50 µg L−1. The developed methodology was tested in cheese and yogurt samples. DNS showed to be an alternative derivatization reagent not only because it produced UV-detectable derivates (214 nm), but also because of its stability, aqueous solubility, high selectivity and sensitivity, reduced impurities, and simple preparation steps.

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Acknowledgements

Authors wish to acknowledge Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES: 001), MSc fellowship granted to JOFM by Programa de Pós-Graduação em Biotecnologia e Monitoramento, UFSCAr (Campus Sorocaba), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 132680/2019-0), and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP 2019/03582-7 and 2018/14425-7) for financial support.

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  1. Departamento de Física, Química e Matemática, Universidade Federal de São Carlos (UFSCar), Sorocaba, SP, Brazil

    Jéssica Oliveira Fernandes Mantoanelli & Elisabete Alves Pereira

  2. Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo (USP), São Paulo, SP, Brazil

    Luís Moreira Gonçalves

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  1. Jéssica Oliveira Fernandes Mantoanelli
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Mantoanelli, J.O.F., Gonçalves, L.M. & Pereira, E.A. Dansyl Chloride as a Derivatizing Agent for the Analysis of Biogenic Amines by CZE-UV. Chromatographia 83, 767–778 (2020). https://doi.org/10.1007/s10337-020-03896-x

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