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Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique

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Abstract

We have developed a method to investigate the interaction between DNA-targeted anthracyclines and DNA in the presence of the drug paclitaxel. It is based on resonance light scattering (RLS) and on the finding that anthracyclines when bound to DNA undergo a dramatic enhancement in their RLS intensities, while paclitaxel does not display such an effect. However, the RLS intensities of the anthracyclines-DNA associates are remarkably enhanced again on addition of paclitaxel. UV-visible spectra reveal interactions between paclitaxel and anthracyclines, but no reaction between paclitaxel and DNA. Consequently, paclitaxel, though not DNA-targeted, can improve the DNA-binding capabilities of anthracyclines. Binding constants between anthracyclines and DNA, and improved efficiency of paclitaxel on the DNA-binding capabilities of anthracyclines were calculated. The DNA binding constants of doxorubicin, epirubicin, and mitoxantrone, respectively, are 4.53 × 105 L mol−1, 6.05 × 105 L mol−1, and 9.47 × 105 L mol−1. The improved values in presence of paclitaxel are 78%, 47% and 19%. We also have investigated the effects of drug concentrations and the order of adding the drugs. Displacement studies (using methylene blue as a competitive agent) provided additional information on the mechanisms of the interaction between paclitaxel and anthracyclines.

A novel resonance light scattering (RLS) method for the investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel has been developed based on the enhanced RLS intensities.

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Acknowledgements

All the authors express their sincere thanks for the financial support from the Guangdong Science and Technology Department (No. 2006B35630009) and the Science Foundation of Shantou University (No. YR09009).

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Authors and Affiliations

  1. Department of Chemistry, Shantou University, Shantou, 515063, China

    Zhanguang Chen, Sihua Qian, Guomin Zhang & Guihua Zeng

  2. Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Xi Chen

  3. Department of Radiology, Peking University Shenzhen Hospital, Shenzhen, 518036, China

    Junhui Chen

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  1. Zhanguang Chen
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  2. Sihua Qian
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  4. Junhui Chen
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Correspondence to Zhanguang Chen.

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Chen, Z., Qian, S., Chen, X. et al. Investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel by resonance light scattering technique. Microchim Acta 177, 67–73 (2012). https://doi.org/10.1007/s00604-011-0747-4

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  • DOI: https://doi.org/10.1007/s00604-011-0747-4

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