Skip to main content
SpringerLink
Log in

Determination of hydrogen peroxide with the aid of peroxidase-like Fe3O4 magnetic nanoparticles as the catalyst

  • Original Paper
  • Published:
Microchimica Acta Aims and scope Submit manuscript

Abstract

As a mimetic peroxidase, Fe3O4 magnetic nanoparticles (MNPs) were prepared and used for the determination of hydrogen peroxide (H2O2) based on their catalytic effect on the oxidation of N,N-diethyl-p-phenylenediamine sulfate (DPD). Fe3O4 MNPs were found to be able to activate H2O2 and oxidize DPD to a colored product with a strong absorption maximum at 550 nm. Under optimized conditions, the absorbance of the product responded linearly to H2O2 concentration in the range from 0.5 to 150 × 10−6 mol L−1 H2O2 with a detection limit as low as 2.5 × 10−7 mol L−1. The method was successfully applied to the determination of H2O2 in rainwater, honey and milk samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Luo Y, Sui Y, Wang X, Tian Y (2008) 2-Chlorophenol induced hydroxyl radical production in mitochondria in carassius auratus and oxidative stress—an electron paramagnetic resonance study. Chemosphere 71:1260

    Article  CAS  Google Scholar 

  2. Wolfbeis OS, Duerkop A, Wu M, Lin Z (2002) A Europium-ion-based luminescent sensing probe for hydrogen peroxide. Angew Chem Int Ed 41:4495

    Article  CAS  Google Scholar 

  3. Manzoori J, Amjadi M, Orooji M (2006) Application of crude extract of kohlrabi (brassica oleracea gongylodes) as a rich source of peroxidase in the spectrofluorometric determination of hydrogen peroxide in honey samples. Anal Sci 22:1201

    Article  CAS  Google Scholar 

  4. Tang B, Zhang L, Xu K (2006) FIA–near-infrared spectrofluorimetric trace determination of hydrogen peroxide using tricarchlorobocyanine dye (Cy.7.Cl) and horseradish peroxidase (HRP). Talanta 68:876

    Article  CAS  Google Scholar 

  5. Chai X, Hou Q, Luo Q, Zhu J (2004) Rapid determination of hydrogen peroxide in the wood pulp bleaching streams by a dual-wavelength spectroscopic method. Anal Chim Acta 507:281

    Article  CAS  Google Scholar 

  6. Wei H, Wang EK (2008) Fe3O4 magnetic nanoparticles as peroxidase mimetics and their applications in H2O2 and glucose detection. Anal Chem 80:2250

    Article  CAS  Google Scholar 

  7. Wang YH Gu HY (2008) Hemoglobin co-immobilized with silver-silver oxide nanoparticles on a bare silver electrode for hydrogen peroxide electroanalysis. Microchim Acta. doi:10.1007/s00604-008-0038-x

  8. Zhang J, Oyama M (2004) A hydrogen peroxide sensor based on the peroxidase activity of hemoglobin immobilized on gold nanoparticles-modified ITO electrode. Electrochim Acta 50:85

    Article  CAS  Google Scholar 

  9. Yuan J, Shiller AM (1999) Determination of subnanomolar levels of hydrogen peroxide in seawater by reagent-injection chemiluminescence detection. Anal Chem 71:1975

    Article  CAS  Google Scholar 

  10. Hanaoka S, Lin, J, Yamada, M (2001) Chemiluminescent flow sensor for H2O2 based on the decomposition of H2O2 catalyzed by cobalt(II)-ethanolamine complex immobilized on resin. Anal Chim Acta 426:57

    Article  CAS  Google Scholar 

  11. George P (1952) Chemical nature of the secondary hydrogen peroxide compounds formed by cytochrome c peroxidase and horseradish peroxidase. Nature 169:612

    Article  CAS  Google Scholar 

  12. Holm TR, George GK, Barcelona M (1987) Fluorometric determination of hydrogen peroxide in groundwater. Anal Chem 59:582

    Article  CAS  Google Scholar 

  13. Zhang GF, Dasgupta PK (1992) Hematin as a peroxidase substitute in hydrogen peroxide determinations. Anal Chem 64:517

    Article  CAS  Google Scholar 

  14. Huang X, Zhu M, Mao L, Shen H (1997) Catalytic determination of hydrogen peroxide by using the molybdenum-porphyrin complex as a mimetic enzyme of peroxidase. Anal Sci 13:145

    Article  Google Scholar 

  15. Aissaoui H, Bachmann R, Schweiger A, Woggon WD (1998) On the origin of the low-spin character of cytochrome P450cam in the resting state-investigations of enzyme models with pulse EPR and ENDOR spectroscopy. Angew Chem Int Ed 37:2998

    Article  CAS  Google Scholar 

  16. Tang B, Du M, Sun Y, Xu H, Shen H (1998) The study and application of biomimic peroxidase ferric 2-hydroxy-1-naphthaldehyde thiosemicarbazone (FeIII-HNT). Talanta 47:361

    Article  CAS  Google Scholar 

  17. Chen Q, Li D, Zhu Q, Zheng H, Xu J (1999) Application of iron tetrasulfonatophthalocyanine as a new mimetic peroxidase in the determination of hydrogen peroxide with p-hydroxyphenylpropionic acid as a substrate. Anal Chim Acta 381:175

    Article  CAS  Google Scholar 

  18. Deng Y, Qi D, Deng C, Zhang X, Zhao D (2008) Superparamagnetic high-magnetization microspheres with an Fe3O4@SiO2 core and perpendicularly aligned mesoporous SiO2 shell for removal of microcystins. J Am Chem Soc 130:28

    Article  CAS  Google Scholar 

  19. Veiseh O, Sun C, Gunn J, Kohler N, Gabikian P, Lee D, Bhattarai N, Ellenbogen R, Sze R, Hallahan A, Olson J, Zhang M (2005) Optical and MRI multifunctional nanoprobe for targeting gliomas. Nano Lett 5:1003

    Article  CAS  Google Scholar 

  20. Lv G, He F, Wang X, Gao F, Zhang G, Wang T, Jiang H, Wu C, Guo D, Li X, Chen B, Gu Z (2008) Novel nanocomposite of nano Fe3O4 and polylactide nanofibers for application in drug uptake and induction of cell death of leukemia cancer cells. Langmuir 24:2151

    Article  CAS  Google Scholar 

  21. Baselt DR, Lee GU, Natesan M, Metzger SW, Sheehan PE, Colton RJ (1998) A biosensor based on magnetoresistance technology. Biosens Bioelectron 13:731

    Article  CAS  Google Scholar 

  22. Gao L, Zhuang J, Nie L, Zhang J, Zhang Y, Gu N, Wang T, Feng J, Yang D, Perrett S, Yan X (2007) Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2:577

    Article  CAS  Google Scholar 

  23. Bader H, Sturzenegger V, Hoigné J (1988) Photometric method for the determination of low concentrations of hydrogen peroxide by the peroxidase catalyzed oxidation of N,N-diethyl-p-phenylenediamine (DPD). Water Res 22:1109

    Article  CAS  Google Scholar 

  24. Chang Q, Zhu L, Yu C, Tang H (2008) Synthesis and properties of magnetic and luminescent Fe3O4/SiO2/Dye/SiO2 nanoparticles. J Lumin 128:1890

    Article  CAS  Google Scholar 

  25. Guo Z, Shen H, Li L (1999) Spectrophotometric determination of hydrogen peroxide and glucose based on hemin peroxidase-like catalyzed oxidation of bromopyrogallol red. Mikrochim Acta 131:171

    CAS  Google Scholar 

  26. Li B, Zhang Z, Jin Y (2001) Chemiluminescence flow biosensor for hydrogen peroxide with immobilized reagents. Sens Actuators B 72:115

    Article  Google Scholar 

  27. Fan C, Gao Q, Zhu D, Wagner G, Li G (2001) An unmediated hydrogen peroxide biosensor based on hemoglobin incorporated in a montmorillonite membrane. Analyst 126:1086

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was partially supports by grant from the National Science Foundation of China (grants Nos. 20877031 and 20677019) and from Hubei Key Laboratory for Catalysis and Material Science in South-Central University for Nationalities.

Author information

Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, People, s Republic of China

    Qing Chang, Lihua Zhu, Guodong Jiang, Chen Yu & Heqing Tang

  2. Hubei Key Laboratory for Catalysis and Material Science, College of Chemistry and Material Science, South-Central University for Nationalities, Wuhan, 430074, People’s Republic of China

    Kejian Deng & Heqing Tang

Authors
  1. Qing Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kejian Deng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lihua Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Guodong Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Chen Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Heqing Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Lihua Zhu or Heqing Tang.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM 1 (DOC 43.0 KB).

ESM 2 (DOC 44.5 KB).

ESM 3 (DOC 39.0 KB).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chang, Q., Deng, K., Zhu, L. et al. Determination of hydrogen peroxide with the aid of peroxidase-like Fe3O4 magnetic nanoparticles as the catalyst. Microchim Acta 165, 299–305 (2009). https://doi.org/10.1007/s00604-008-0133-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00604-008-0133-z

Keywords

Search

Navigation